Yan Zhang, Gang Cao, Qing-gong Yuan, Jun-hui Li, Wen-Bin Yang

Oncology Research, Vol.25, No.4, pp. 537-544, 2017, DOI:10.3727/096504016X14756640150695

Abstract Empty spiracles homeobox 2 (EMX2) is a homeodomain-containing transcription factor that plays an essential role in tumorigenesis. However, to the best of our knowledge, the role of EMX2 in human colorectal cancer (CRC) is still unclear. Thus, the aim of this study was to investigate the expression and role of EMX2 in CRC. Our results demonstrated that the expression of EMX2 was greatly decreased in CRC tissues and cell lines. Overexpression of EMX2 significantly inhibited the proliferation in vitro and CRC tumor growth in nude mice. In addition, EMX2 also inhibited the migration and invasion More >

Ping Zhao^*, Hai-Tao Guan^†, Zhi-Jun Dai^†, Yu-Guang Ma^†, Xiao-Xu Liu^†, Xi-Jing Wang^†

Oncology Research, Vol.25, No.1, pp. 115-122, 2017, DOI:10.3727/096504016X14732772150181

Abstract Tripartite motif-containing protein 37 (TRIM37), a new member of the RING-B-box-coiled-coil (RBCC) subfamily of zinc finger proteins, was found to be involved in the development and progression of several cancers. However, the expression pattern and biological functions of TRIM37 in colorectal cancer (CRC) remain unknown. Therefore, in the present study, we examined the expression pattern of TRIM37 in CRC and investigated the function of TRIM37 in the progression of CRC. Our results showed that TRIM37 expression was upregulated in CRC cell lines. Knockdown of TRIM37 inhibited CRC cell proliferation and tumor growth in vivo. Furthermore, More >

Tao Xie^*, Yao Li^†, Shi-Lei Li^*, Hai-Feng Luo^‡

Oncology Research, Vol.24, No.6, pp. 447-453, 2016, DOI:10.3727/096504016X14685034103590

Abstract Although astragaloside IV exhibits anti-inflammation, immunoregulatory, and anticancer properties, the chemosensitization effects of astragaloside IV in colorectal cancer have never been reported. Our study tested whether astragaloside could increase cisplatin sensitivity in colorectal cancer. CCK-8 assay was used to measure the cell viability of colorectal cancer cells. Quantitative real-time PCR and Western blot were performed to determine the mRNA and protein expression, respectively. Our data revealed that astragaloside IV administration significantly suppressed the cell growth of colorectal cancer cells, whereas no obvious cytotoxicity of astragaloside IV was observed in nonmalignant colonic cells. In addition, combined… More >

Ping Zhao^*, Hai-Tao Guan^†, Zhi-Jun Dai^†, Yu-Guang Ma^†, Xiao-Xu Liu^†, Xi-Jing Wang^†

Oncology Research, Vol.24, No.6, pp. 437-445, 2016, DOI:10.3727/096504016X14685034103554

Abstract Sparc/osteonectin, cwcv, and kazal-like domains proteoglycan (testican) 1 (SPOCK1), known as testican-1, were found to be involved in the development and progression of tumors. However, in colorectal cancer (CRC), the expression pattern of SPOCK1 and its functional role remain poorly investigated. In the present study, we explored the role of SPOCK1 in CRC. Our results demonstrated that SPOCK1 is overexpressed in CRC cell lines. SPOCK1 silencing significantly inhibited the proliferation in vitro and the tumor growth in vivo. Furthermore, SPOCK1 silencing significantly attenuated the migration/invasion by reversing the EMT process in CRC cells. Finally, knockdown More >

Hongtao Ren^*, Zhongwei Wang^*, Shuqun Zhang^*, Hongbing Ma^*, Yali Wang^*, Lijun Jia^*, Yiming Li^†

Oncology Research, Vol.23, No.5, pp. 249-256, 2015, DOI:10.3727/096504016X14562725373716

Abstract Interleukin-17A (IL-17A) plays a significant role in many inflammatory diseases and cancers. The aim of this study is to investigate the effect of IL-17A on the invasiveness of colorectal cancer. In the study, we found that IL-17A could promote the migration and invasion of colorectal cancer cells. Furthermore, after being treated with IL-17A, the expression and activity of matrix metalloproteinase 2 (MMP-2) and MMP-9 were upregulated. Moreover, the nuclear/overall fractions and DNA-binding activity of p65 and p50 were dramatically elevated by IL-17A. Pretreatment with a nuclear factor-kB (NF-kB) inhibitor (PDTC) or PI3K/AKT inhibitor (LY294002) was More >

Yaohua Fan^*†1, Xiyan Zhu^*1, Qiuping Lan^*, Fang Lou^*, Yu Zheng^*, Haizhou Lou^*, Yong Fang^*, Wei Jin^*, Hongming Pan^*, Kaifeng Wang^*‡

Oncology Research, Vol.23, No.5, pp. 219-228, 2015, DOI:10.3727/096504016X14567549091260

Abstract Radiofrequency ablation (RFA) is a minimally invasive technology for the treatment of liver malignancies and is used as an adjuvant therapy in patients with colorectal liver metastasis (CLM). This study enrolled a total of 49 CLM patients who underwent RFA treatment. Univariate and multivariate analyses were performed using the log-rank test and Cox proportional hazard model, respectively. Univariate analysis showed that OS was closely correlated with tumor size, frequency of RFA treatment, resection of the liver lesion, and CEA levels before RFA (p<0.05). Multivariate analysis revealed that resection of CLM lesions after RFA, frequency of RFA More >

Jianyu Zhou^*, Xiaorong Li^*, Meirong Wu^†, Changwei Lin^*, Yihang Guo^*, Buning Tian^*

Oncology Research, Vol.23, No.6, pp. 303-309, 2015, DOI:10.3727/096504016X14567549091305

Abstract Emerging evidence has identified the vital role of long noncoding RNAs (lncRNAs) in the development of colorectal cancer. In this study, we aimed to investigate the role of lncRNA gastric carcinoma highly expressed transcript 1 (GHET1) in colorectal cancer. We analyzed the expression of GHET1 in colorectal cancer (CRC) tissues by using ISH. We found that GHET1 expression was significantly increased in the CRC samples compared with adjacent tissues. Furthermore, the cancer tissues had higher GHET1 mRNA levels than their matched adjacent tissues. GHET1 expression was also significantly increased in the CRC cell lines compared More >

Huiqing Lv^*, Zhongmin Zhang^†, Yaoxia Wang^†, Chenglin Li^†, Weihong Gong^†, Xin Wang^†

Oncology Research, Vol.23, No.6, pp. 283-290, 2015, DOI:10.3727/096504016X14562725373833

Abstract Colorectal cancer (CRC) is the third most common malignancy with high mortality around the world. However, the biological mechanism of CRC carcinogenesis is not completely known. In the present study, we determined the role of miR-92a in the regulation of CRC cell motility. Expression of miR-92a is aberrantly upregulated in human CRC tissues and cultured cells, as shown by RT-PCR analysis. The effects of miR-92a on the proliferation and migration of human CRC SW620 and LoVo cells were measured by CCK-8 and Transwell assay, respectively. Results showed that the proliferation and migration capacity of both… More >