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  • Open Access

    ARTICLE

    Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver

    CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN

    BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033

    Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 More >

  • Open Access

    ARTICLE

    RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct

    PABLO A. VALDECANTOS, MARTÍN E. ARGAÑARAZ, CARLOS M. ABATE, DORA C. MICELI

    BIOCELL, Vol.28, No.3, pp. 287-297, 2004, DOI:10.32604/biocell.2004.28.287

    Abstract As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses More >

  • Open Access

    ARTICLE

    Cantilever Arrays for Multiplexed Mechanical Analysis of Biomolecular Reactions

    Min Yue1,1, Jeanne C. Stachowiak1,1,2,2, Arunava Majumdar1,1,3,3

    Molecular & Cellular Biomechanics, Vol.1, No.3, pp. 211-220, 2004, DOI:10.3970/mcb.2004.001.211

    Abstract Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in More >

  • Open Access

    ARTICLE

    Encapsulation of Pt-labelled DNA Molecules inside Carbon Nanotubes

    Daxiang Cui1, Cengiz S. Ozkan2, Sathyajith Ravindran3, Yong Kong1, Huajian Gao1

    Molecular & Cellular Biomechanics, Vol.1, No.2, pp. 113-122, 2004, DOI:10.3970/mcb.2004.001.113

    Abstract Experiments on encapsulating Pt--labelled DNA molecules inside multiwalled carbon nanotubes (MWCNT) were performed under temperature and pressure conditions of 400K and 3 Bar. The DNA-CNT hybrids were purified via agarose gel electrophoresis and analyzed via high resolution transmission electron microscopy (HR-TEM) and energy dispersive X-ray spectroscopy (EDX). The results showed that the Pt-labelled DNA molecules attached to the outside walls of CNTs could be removed by electrophoresis. The HR-TEM and EDX results demonstrated that 2-3% of the Pt-labelled DNA molecules were successfully encapsulated inside the MWCNTs. The experimental study complements our previous molecular dynamics simulations More >

  • Open Access

    ARTICLE

    Structure of the kidney of Bufo arenarum: Intermediate segment, distal tubule and collecting tubule

    A. Farías, G.N. Hermida, L.E. Fiorito

    BIOCELL, Vol.27, No.1, pp. 19-28, 2003, DOI:10.32604/biocell.2003.27.019

    Abstract The ultrastructure of the intermediate segment (IS), distal tubule and collecting tubule (CT) of the south american toad Bufo arenarum, was studied by light and transmission electron microscopy. The IS is composed of cubical ciliated cells which propel the urine along the renal tubule. The distal tubule is divided into two portions: the early distal tubule (EDT) and the late distal tubule (LDT). The EDT is characterized by only one type of cells with well developed basolateral interdigitations and numerous elongated mitochondria, which are oriented normal to the basal surface. The “macula densa - like” is… More >

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