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  • Open Access

    ARTICLE

    Cell proliferation of the ileum intestinal mucosa of diabetic rats treated with ascorbic acid

    JACQUELINE NELISIS ZANONI, RENATA VIRGINIA FERNANDES PEREIRA

    BIOCELL, Vol.32, No.2, pp. 163-168, 2008, DOI:10.32604/biocell.2008.32.163

    Abstract The objective of this work was to evaluate the effect of the ascorbic acid supplementation on the cellular proliferation on the ileum mucosa of diabetic rats. Fifteen 90-days rats were divided in the groups: control, diabetic and diabetic supplemented with ascorbic acid (DA). Two hours prior the sacrifice, they were injected with Vincristin. Semi-seriate histological cuts stained with HE were accomplished. About 2500 crypt cells from the intestinal mucosa were counted in order to obtain the metaphasic indexes. The height and depth of 30 villi and 30 crypts were measured for each animal, respectively. The… More >

  • Open Access

    ARTICLE

    Effects of the ascorbic acid supplementation on NADH-diaphorase myenteric neurons in the duodenum of diabetic rats

    MARLI APARECIDA DOS SANTOS PEREIRA, MARIA CLAÚDIA BAGATIN, JACQUELINE NELISIS ZANONI

    BIOCELL, Vol.30, No.2, pp. 295-300, 2006, DOI:10.32604/biocell.2006.30.295

    Abstract We assessed the ascorbic acid (AA) supplementation on the myenteric neurons in the duodenum of rats. Fifteen rats with 90 days of age were divided into three groups: control (C), diabetics (D) and ascorbic acid treated diabetics (DA). After 120 days of daily treatment with AA, the duodenum was submitted to the NADH-diaphorase (NADH-d) histochemical technique, which allowed us to evaluate the neuronal density in an area of 8.96 mm2 for each duodenum, and also to measure the cellular profile area of 500 neurons per group. The supplementation promoted an increase on AA levels. The neuronal More >

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