Ping He^*, Hong-xia Zhang^†, Chang-yu Sun^*, Chun-yong Chen^‡, He-qing Jiang^*

Oncology Research, Vol.24, No.1, pp. 25-32, 2016, DOI:10.3727/096504016X14575597858609

Abstract The SASH1 (SAM- and SH3-domain containing 1) gene, a member of the SLY (SH3 domain containing expressed in lymphocytes) family of signal adapter proteins, has been implicated in tumorigenesis of many types of cancers. However, the role and mechanism of SASH1 in the invasion and metastasis of hepatocarcinoma are largely unknown. In this study, we investigated the role and mechanism of SASH1 in the invasion and metastasis of hepatocarcinoma. Our results showed that SASH1 was lowly expressed in hepatocarcinoma cell lines. The in vitro experiments showed that overexpression of SASH1 inhibited the proliferation and migration/invasion More >

Wei Zong^*, Chen Yu^†, Ping Wang^*, Lei Dong^‡

Oncology Research, Vol.24, No.1, pp. 17-23, 2016, DOI:10.3727/096504016X14570992647203

Abstract The epithelial–mesenchymal transition (EMT) is considered to be one of the critical steps in gastric cancer cell invasion and metastasis. SAM- and SH3-domain containing 1 (SASH1), a member of the SLY family of signal adapter proteins, is a candidate for tumor suppression in several cancers. However, the biological role of SASH1 in gastric cancer remains largely unknown. Therefore, the purpose of this study was to investigate the impact of SASH1 on the biological behavior of gastric cancer cells treated with transforming growth factor (TGF)-β1. In the current study, we provide evidence that SASH1 was lowly More >

Si Jun Park^*1, Bo Ram Lee^*1, Hyeng-Soo Kim^*, Young Rae Ji^*, Yong Hun Sung^*, Kwang ShikChoi^*, Hum Dai Park^†, Sung-Hyun Kim^*, Myoung Ok Kim^‡, Zae Young Ryoo^*

Oncology Research, Vol.23, No.3, pp. 89-98, 2015, DOI:10.3727/096504015X14496932933539

Abstract In the present study, we found that lung cancer cell line (H460 cells) expressing Tet1 showed higher levels of adhesion, and Tet1 inhibited H460 cell proliferation. In addition, these cells showed a significantly reduced ability of collagen degradation and Smad2/3 phosphorylation compared to controls. Furthermore, vimentin was found to be highly expressed in larger metastatic cancer area. Tet1 overexpression was reduced in the epithelial marker E-cadherin. Moreover, Tet1 repressed cancer cell metastasis in nude mice. Collectively, these findings suggest that Tet1 expression plays a critical role in metastasis of lung cancer cells by suppression of More >

Jianyu Zhou^*, Xiaorong Li^*, Meirong Wu^†, Changwei Lin^*, Yihang Guo^*, Buning Tian^*

Oncology Research, Vol.23, No.6, pp. 303-309, 2015, DOI:10.3727/096504016X14567549091305

Abstract Emerging evidence has identified the vital role of long noncoding RNAs (lncRNAs) in the development of colorectal cancer. In this study, we aimed to investigate the role of lncRNA gastric carcinoma highly expressed transcript 1 (GHET1) in colorectal cancer. We analyzed the expression of GHET1 in colorectal cancer (CRC) tissues by using ISH. We found that GHET1 expression was significantly increased in the CRC samples compared with adjacent tissues. Furthermore, the cancer tissues had higher GHET1 mRNA levels than their matched adjacent tissues. GHET1 expression was also significantly increased in the CRC cell lines compared More >

Yunpeng Ren, J. K. Chen^*, Yuwen Zhang

Frontiers in Heat and Mass Transfer, Vol.3, No.2, pp. 1-7, 2012, DOI:10.5098/hmt.v3.2.3001

Abstract Heat transfer in a copper film irradiated by a femtosecond (fs) laser pulse train and by an integrated dual laser beam of a nanosecond pulse with a fspulse train was studied using the semi-classical two-temperature model. The critical point model with three Lorentzian terms was employed to characterize transient optical properties for the laser energy deposition. The effects of pulse number and separation time on the thermal response were investigated. The results showed that with the same total energy in a fs-pulse train, more pulses for shorter separation time, e.g., 1 ps, and fewer pulses More >

Jing Huang, Kapil Baheti, J. K. Chen^*, Yuwen Zhang

Frontiers in Heat and Mass Transfer, Vol.2, No.1, pp. 1-10, 2011, DOI:10.5098/hmt.v2.1.3005

Abstract An axisymmetric model for thermal transport in thin metal films irradiated by an ultrashort laser pulse was developed. The superheating phenomena including preheating, melting, vaporization and re-solidification were modeled and analyzed. Together with the energy balance, nucleation dynamics was employed iteratively to track the solid-liquid interface and the gas kinetics law was used iteratively to track the liquid-vapor interface. The numerical results showed that higher laser fluence and shorter pulse width lead to higher interfacial temperature, larger melting and ablation depths. A simplified 1-D model could overestimate temperature response and ablation depth due to the More >

Nan Shen^1,2, Dabajyoti Datta¹, Chris B. Schaffer^1,3,4,5, Eric Mazur^1,6

Molecular & Cellular Biomechanics, Vol.2, No.1, pp. 17-26, 2005, DOI:10.3970/mcb.2005.002.017

Abstract Analysis of cell regulation requires methods for perturbing molecular processes within living cells with spatial discrimination on the nanometer-scale. We present a technique for ablating molecular structures in living cells using low-repetition rate, low-energy femtosecond laser pulses. By tightly focusing these pulses beneath the cell membrane, we ablate cellular material inside the cell through nonlinear processes. We selectively removed sub-micrometer regions of the cytoskeleton and individual mitochondria without altering neighboring structures or compromising cell viability. This nanoscissor technique enables non-invasive manipulation of the structural machinery of living cells with several-hundred-nanometer resolution. Using this approach, we More >