García-Zambrano² Eduardo A, Adriana Gutiérrez-Díez, Gilberto E Salinas-García, Elizabeth Cárdenas-Cerda, Rigoberto E Vázquez-Alvarado, Francisco Zavala-García, Jesús Martínez de la Cerda

Phyton-International Journal of Experimental Botany, Vol.75, pp. 125-135, 2006, DOI:10.32604/phyton.2006.75.125

Abstract The objective of this investigation was to develop and apply molecular markers (type RAPD) for estimating the genetic diversity of 100 Opuntia accessions from the Germoplasma Bank of the FAUANL. The molecular data were evaluated by multivariate analysis using the methods of Ward and UPGMA. These results were compared with phenotypic data taken from the same accessions, already evaluated by the above mentioned methods. The protocol was modified for the extraction of the Opuntia DNA to reduce the quantity of polysaccharides and their influence in its quality. Later on, a protocol was developed for the generation of molecular markers of… More >

Tantasawat PA, K Lunwongsa, T Linthaisong, P Wirikitgul, N Campatong, N Talpolkrung, A Tharapreuksapong, O Poolsawat, A Khairum, A Sorntip, C Kativat

Phyton-International Journal of Experimental Botany, Vol.87, pp. 162-170, 2018, DOI:10.32604/phyton.2018.87.162

Abstract Genetic diversity and relatedness of 17 water primrose (Ludwigia hyssopifolia) accessions in Thailand were estimated using morphological characters and random amplified polymorphic DNA (RAPD) markers. Eight morphological characters were diverse among the accessions. However, some accessions could not be distinguished from one another based on these morphological characters alone. Unweighted pair-group arithmetic average (UPGMA) analysis of these characters separated these 17 accessions into 2 major clusters. Among the 5 RAPD primers used, a total of 68 fragments (150 to 2000 bp) were amplified, showing a polymorphism percentage of 80%. The polymorphic information content (PIC) among accessions varied from 0.31 to… More >

García-Zambrano EA¹, MC Ojeda-Zacarías¹, JG Gutiérrez-Soto¹, SR Sinagawa-García¹, A Gutiérrez-Díez¹, J Martínez-de la Cerda¹, JA Pedroza-Flores¹, C Gallegos-Vázquez², JI García-Zambrano³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged… More >