Adam P. Klausner1, Corey M. Johnson1, Aaron B. Stike1, John E. Speich2, Vikram Sabarwal3, Amy S. Miner1, MaryEllen Cleary, Harry P. Koo1, Paul H. Ratz3
Canadian Journal of Urology, Vol.18, No.2, pp. 5608-5614, 2011
Abstract Introduction: The purpose of this investigation was to determine whether prostaglandin E2 (PGE2) is produced by rabbit detrusor muscle that is free of urothelium, and to demonstrate that PGE2 is responsible for the generation of spontaneous rhythmic contractions (SRC).
Materials and Methods: A bioassay was conducted in which the contraction frequency of rabbit detrusor strips was compared before and after the addition of superfusate obtained from incubated sections of rabbit detrusor. Specificity of the response was assessed using SC-51089, a PGE2 (EP1) receptor antagonist. The effects on tension development were also tested in artery segments treated with… More >