Hernández-Quintero JD¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, M Gómez-Martínez², R Rodríguez-Herrera⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 107-112, 2015, DOI:10.32604/phyton.2015.84.107

Abstract The genus Dasylirion is a group of plants typically present in the Chihuahuan Desert, perennial, with a dioecious sexual behavior and commonly called sotoles. This genus has been little studied from the biological point of view, and the bases of its reproductive response remain unknown. In this work we studied the chromosome number and meiotic response of Dasylirion cedrosanum in the county of Saltillo, Coahuila, located at the North East of Mexico. For the preparation of mitotic chromosomes, we used a technique based on enzymatic treatment with pectolyase and cellulase, as well as staining with acetocarmin… More >

Rodríguez de León JI¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, F Ramírez-Godina², V Robledo-Torres², M Gómez-Martínez², G Hernández-Guzmán⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 101-106, 2015, DOI:10.32604/phyton.2015.84.101

Abstract The cultivated husk tomato (Physalis ixocarpa) (2n = 2x = 24) is native from Mexico and Central America and shows a wide genetic variation. Presently, it is the fourth horticultural crop in cultivation surface in Mexico. The working team of this research previously developed an autotetraploid population by using colchicine. The objectives of the present work were to analyze the ploidy level and meiotic behavior of the subsequent generations (C3, C4, C5, C6) from the original (C2) composed only by plants with the duplicated genome from the Rendidora cultivar, and to determine pollen viability. As a… More >

Gracieli DALLA NORA, Solange Bosio TEDESCO, Tamara PASTORI, Viviane Dal-Souto FRESCURA^*, Nerinéia Dalfollo RIBEIRO, Haywood Dail LAUGHINGHOUSE IV, Sidinei José LOPES, Maria Teresa SCHIFINO-WITTMANN

BIOCELL, Vol.38, No.1, pp. 33-38, 2014, DOI:10.32604/biocell.2014.38.033

Abstract The aims of this study were to determine the meiotic behavior and to estimate pollen grains viability in bean (Phaseolus vulgaris L.) cultivars. Flower buds were collected during different developmental stages of the Mesoamerican bean cultivars IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662 and Pérola, and the Andean bean cultivar Iraí, grown in a greenhouse. The meiotic index was determined by anther squashing of material fixed in absolute ethanol-glacial acetic acid (3:1) and stained with acetic orcein. No meiotic abnormalities were observed and the meiotic indices were high for all cultivars, indicating More >

T.I. Zohdi¹

CMES-Computer Modeling in Engineering & Sciences, Vol.98, No.3, pp. 261-277, 2014, DOI:10.32604/cmes.2014.098.261

Abstract A widespread use of lasers is for the ablation of biological tissue, in particular for dermal applications involving the removal of cancerous tissue, skin spots, aged skin and wrinkles. For a laser to ablate tissue, the power intensity must be sufficiently high to induce vaporization/burning of the target material. However, if performed improperly, the process can cause excessive microscale thermal injuries to surrounding healthy tissue. This motivates the present work, which attempts to develop and assemble simple models for the primary heat transfer mechanisms that occur during the process. First, in order to qualitatively understand… More >

Tamilselvan SUBRAMANI^1,2, Suresh RAO², Kamatchiammal SENTHILKUMAR³, Soundararajan PERIASAMY⁴, Noorjahan Banu ALITHEEN¹

BIOCELL, Vol.37, No.3, pp. 71-76, 2013, DOI:10.32604/biocell.2013.37.071

Abstract The present study demonstrates that the expression of c-Jun and c-Fos are elevated in gingival fibroblast cells treated with angiotensin II and cyclosporine. The healthy human gingival tissues were collected and gingival fibroblasts were isolated and cultured. We used RT-PCR and Western blot analysis to identify the expression of c-Jun and c-Fos in cyclosporine and angiotensin II treated human gingival fibroblast cells. We found that angiotensin II in combination with cyclosporine induces c-Jun and c-Fos expressions significantly; however, the angiotensin II antagonist losartan inhibits the expression of c-Jun and c-Fos (p < 0.01). The data More >

GABRIEL CÉCCOLI^*1, JULIO C. RAMOS¹, LEANDRO I. ORTEGA² , JUAN M. ACOSTA¹, MARIEL G. PERRETA¹

BIOCELL, Vol.35, No.1, pp. 9-17, 2011, DOI:10.32604/biocell.2011.35.009

Abstract Chloris gayana Kunth is a grass species valuable as forage which was introduced into Argentina to be used as pasture in saline soils of subtropical and warm-temperate zones, given its good adaptability to drought, salinity and mild freezing. However, its tolerance varies according to the cultivar. In tetraploid cultivars, important reductions in yield have been observed. Here, a study of the variations produced on the root and stem system by salinity at different NaCl concentrations (0, 150 y 250 mM) was performed in the Boma cultivar, with the aim of determining the anatomical and morphological alterations More >

ROMINA J. BEVACQUA, RAFAEL FERNANDEZ-MARTIN, DANIEL F. SALAMONE

BIOCELL, Vol.35, No.1, pp. 1-7, 2011, DOI:10.32604/biocell.2011.35.001

Abstract Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid… More >

Rojas-Herrera R¹, M Zamudio-Maya¹, L Arena-Ortiz², RC Pless³, A O’Connor-Sánchez⁴

Phyton-International Journal of Experimental Botany, Vol.80, pp. 231-240, 2011, DOI:10.32604/phyton.2011.80.231

Abstract Mexico counts among the five countries with the highest biodiversity in the world. In the Yucatán Peninsula, there are aquatic ecosystems with a very special microbial diversity. These ecosystems are essential for the ecological equilibrium of the region, and are seriously threatened by human activities. Access and knowledge of the microbial resources of these environments have an enormous scientific interest, and could potentially result in biotechnological products which could lead to more efficient and environmentally friendly processes; it could also offer a full arsenal of microorganisms and/or novel molecules to the local and world industry… More >

Hernández-León R, I Velázquez-Sepúlveda, MC Orozco-Mosqueda, G Santoyo

Phyton-International Journal of Experimental Botany, Vol.79, pp. 133-139, 2010, DOI:10.32604/phyton.2010.79.133

Abstract Soil is a complex system that includes a great number and diversity of microorganisms. Until recently, only a small percentage of the bioma was known and could be studied. Currently, it is possible to have a deeper knowledge of all that unknown genomic material with the development of new tools, like metagenomics. New molecules have been discovered with various biotechnological applications, and knowledge of the diverse microbiological interactions in several environments, some of them with extreme life conditions, is much higher. We analyze the most recent literature in the field of metagenomics in this study, More >

R. M. Ardito Marretta^*, F. Ales^†

Molecular & Cellular Biomechanics, Vol.7, No.4, pp. 225-266, 2010, DOI:10.3970/mcb.2010.007.225

Abstract In this paper, cell cycle in higher eukaryotes and their molecular networks signals both inG1/SandG2/Mtransitions are replicatedin silico. Biochemical kinetics, converted into a set of differential equations, and system control theory are employed to design multi-nested digital layers to simulate protein-to-protein activation and inhibition for cell cycle dynamics in the presence of damaged genomes. Sequencing and controlling the digital process of four micro-scale species networks (p53/Mdm2/DNA damage, p21mRNA/cyclin-CDK complex, CDK/CDC25/wee1/ SKP2/APC/CKI and apoptosis target genes system) not only allows the comprehension of the mechanisms of these molecule interactions but paves the way for unraveling the… More >