Yang Zhao, Wenxia Chen, Weiliang Zhu, Hui Meng, Jie Chen, Jian Zhang

Oncology Research, Vol.25, No.4, pp. 511-522, 2017, DOI:10.3727/096504016X14756226781802

Abstract The purpose of this study was to identify the role of interferon regulatory factor 7 (IRF7) in the bone metastasis of prostate cancer. Herein we demonstrated the lower expression of IRF7 in bone metastases of prostate cancer. Overexpression of IRF7 in prostate cancer cells had a marked effect on inhibiting bone metastases but not on tumor growth in xenograft nude mice. While in vitro, upregulation of IRF7 had little effect on the malignant phenotype of prostate cancer cells including proliferation, apoptosis, migration, and invasion. However, prostate cancer cells overexpressing IRF7 significantly enhanced the activity of More >

Cheng Xu^*1, Lu Cao^*1, Jianhua Liu^†1, Zhongrun Qian^‡, Yu Peng^§, Wenjing Zhu^§, Yongming Qiu^‡, Yingying Lin^‡¶

Oncology Research, Vol.25, No.3, pp. 407-415, 2017, DOI:10.3727/096504016X14743350548249

Abstract Elevated circulating asparaginyl endopeptidase (AEP), a novel lysosomal protease, has been found in breast cancer, and AEP is thus considered to be a prognostic factor in this disease. However, the pathological functions of circulating AEP in the development of breast cancer and the potential of AEP-targeted therapy remain unclear. We used MMTV-PyVmT transgenic mice, which spontaneously develop mammary tumors. Western blotting showed overexpression of AEP in both primary tumor tissue and lung metastases compared to their normal counterparts. Moreover, the concentration of circulating AEP gradually increased in the serum during the development of mammary tumors. More >

Kenan Izgi^1,3, Banu Iskender^2,3, Cagri Sakalar^2,3, Aslihan Arslanhan^1,3, Berkay Saraymen^1,4, Halit Canatan^2,3

European Cytokine Network, Vol.26, No.2, pp. 46-56, 2015, DOI:10.1684/ecn.2015.0365

Abstract Rationale: Organ- or tissue-specific antigens produced by normal tissue or by cancer cells could be used in cancer immunotherapy, to target the tumor. In our previous study, we induced T-cell-mediated, bladder-specific autoimmunity by targeting the bladder-specific protein Uroplakin 3A (UPK3A). UPK3A is a well-chosen target for developing an autoimmune response against bladder cancer since the antigen is also expressed in bladder tumors. To use this peptide, which was derived from the UPK3A protein in a bladder cancer vaccine study, it is necessary to induce a strong immune response. In this study, we aimed to develop… More >

Abdullah Alyoussef

European Cytokine Network, Vol.26, No.2, pp. 38-45, 2015, DOI:10.1684/ecn.2015.0364

Abstract Purpose: Atopic dermatitis (AD) is a chronically relapsing, pruritic, eczematous skin disorder accompanying allergic inflammation. AD is triggered by oxidative stress and immune imbalance. The effect of oral arjunolic acid (AA) on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in mice was investigated. Methods: Repeated epicutaneous application of DNCB to the ear and shaved dorsal skin of mice was performed to induce AD-like symptoms and skin lesions: 250mg/kg AA was given orally for three weeks to assess its anti-pruritic effects. Serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-4, IL-6, IL-10, immunoglobulin (Ig)E and caspase-3 were assessed by More >

Jian-lin Yang^*1, Ye Qin^*1, Liang Li^†, Chu-yu Cao^*, Qing Wang^*, Qian Li^*, Ya-feng Lv^*, Yanlin Wang^*

Oncology Research, Vol.23, No.1-2, pp. 79-86, 2015, DOI:10.3727/096504015X14478843952942

Abstract In the process of tumor cell apoptosis induced by specific regents, calreticulin (CRT) was transferred from endoplasmic reticulum (ER) onto the cell membrane. These tumor cells, when used as the cellular vaccine to immunize experimental animals, could initiate effective antitumor immunoresponse against homologous tumor cells. This is referred to as immunogenic cell death. Lidamycin (LDM) is an enediyne antibiotic, which has extremely potent cytotoxicity to cancer cells. In this study, the mouse melanoma B16-F1 cancer cells were used to investigate the ability of LDM in promoting immunogenic cell death. Our data showed that LDM could… More >

Huijie Jia^*†1, Tiesuo Zhao^‡§1, Di Zou^¶, Xiaolong Jia^§, Ji Gao^#, Xiangfeng Song^‡§

Oncology Research, Vol.23, No.6, pp. 321-326, 2015, DOI:10.3727/096504016X14570992647041

Abstract Synthetic CpG oligodeoxynucleotides (ODNs), as TLR9 agonists, have been found to play a possible role in antitumor effect. In order to determine the effect of YW002, known as a C-type CpG ODN, on the treatment of hepatocellular carcinoma (HCC), which is one of the most aggressive carcinomas, we chose to inject YW002 at the doses of 12.5 μg and 25 μg per mouse 7 days post-tumor challenge. The survival rate of mice was recorded every day. On day 14 postinjection, five mice in each group were bled and randomly sacrificed. The level of IFN-γ or… More >

Alton G. Sutter¹, Arun P. Palanisamy¹, Justin D. Ellet¹, Michael G. Schmidt², Rick G. Schnellmann^3,4, Kenneth D. Chavin¹

European Cytokine Network, Vol.25, No.4, pp. 69-76, 2014, DOI:10.1684/ecn.2015.0359

Abstract Steatotic livers are more sensitive to ischemia/reperfusion (I/R) and are thus routinely rejected for transplantation because of their increased rate of primary nonfunction (PNF). Lean livers have less I/R-induced damage and inflammation due to Kupffer cells (KC), which are protective after total, warm, hepatic I/R with associated bowel congestion. This protection has been linked to KC-dependent expression of the potent anti-inflammatory cytokine interleukin-10 (IL-10).We hypothesized that pretreatment with exogenous IL-10 would protect the steatotic livers of genetically obese (ob/ob) mice from inflammation and injury induced by I/R. Lean and ob/ob mice were pretreated with either More >

Michel Drouet, Nancy Grenier, Francis Hérodin

European Cytokine Network, Vol.23, No.2, pp. 56-63, 2012, DOI:10.1684/ecn.2012.0306

Abstract We have re-evaluated the benefit of using erythropoietin (Epo) as a pleiotropic cytokine to counteract hematological and extra-hematological toxicity following lethal irradiation. B6D2F1 mice were exposed to a dose of 9 Gy gamma radiation resulting in 90% mortality at 30 days, and then injected with stem cell factor, FLT-3 ligand, thrombopoietin and interleukin-3 [i.e. SFT3] at two and 24 hours with or without Epo (1,000 IU/kg) at 2 hours and day 8. As controls, two groups of irradiated mice were given only Epo or Phosphate-buffered saline. Epo synergized with SFT3 to rescue lethally-irradiated mice from More >

Carlos Zgheib^1,a, Fouad Anthony Zouein^1,a, Mazen Kurdi^1,2, George Warren Booz¹

European Cytokine Network, Vol.23, No.4, pp. 191-197, 2012, DOI:10.1684/ecn.2012.0319

Abstract Recent evidence suggests that the IL-6 family cytokine, leukemia inhibitory factor (LIF) is produced by cardiac cells under stress conditions including myocardial infarction and heart failure. Additionally, short-term delivery of LIF has been shown to have preconditioning effects on the heart and to limit infarct size. However, cell culture studies have suggested that LIF may exert harmful effects on cardiac myocytes, including pathological hypertrophy and contractile dysfunction. Long-term effects of LIF on the heart in vivo have not been reported and were the focus of this study. Adult male mice were injected daily with LIF… More >

MIRIAM TERUEL^1,2, JAQUELINE D’ERCOLE¹ AND RODOLFO CATALANO²

BIOCELL, Vol.35, No.1, pp. 29-34, 2011, DOI:10.32604/biocell.2011.35.029

Abstract Benzimidazole compounds are used in both humans and animals for controlling helminth parasites. Albendazole has teratogenic effects attributed to its active metabolite albendazole sulphoxide. The aim of this work was to evaluate the effect of the latter compound when administered to pregnant CF1 mice during the preimplantation period. Females were superovulated by intraperitoneal injection of 10 IU of eCG and 10 IU of hCG (48h later) and were paired with males of proven fertility. Albendazole sulphoxide (200 mg/kg) was orally administered by gavages at day 1, 2 or 3 of pregnancy; the control group received More >