Zhihai Wang^*, Kui Liao^†, Wenqi Zuo^*, Xueliang Liu^*, Zhili Qiu^*, Zhitao Gong^*, Chuan Liu^*, Quan Zeng^*, Yi Qian^*, Liang Jiang^*, Youquan Bu^‡, Suling Hong^*, Guohua Hu^*

Oncology Research, Vol.25, No.1, pp. 123-136, 2017, DOI:10.3727/096504016X14732772150226

Abstract NFBD1, a signal amplifier of the p53 pathway, is vital for protecting cells from p53-mediated apoptosis and the early phase of DNA damage response under normal culture conditions. Here we investigated its expression in patients with nasopharyngeal carcinoma (NPC), and we describe the biological functions of the NFBD1 gene. We found that NFBD1 mRNA and protein were more highly expressed in NPC tissues than in nontumorous tissues. To investigate the function of NFBD1, we created NFBD1-depleted NPC cell lines that exhibited decreased cellular proliferation and colony formation, an increase in their rate of apoptosis, and More >

Kang Zhu^*, Ying He^†, Cui Xia^*, Jing Yan^*, Jin Hou^*, Demin Kong^*, Yeye Yang^*, Guoxi Zheng^*

Oncology Research, Vol.24, No.3, pp. 145-151, 2016, DOI:10.3727/096504016X14611963142290

Abstract Nasopharyngeal carcinoma (NPC) is a highly metastatic cancer, frequently occurring in Southeast Asia and Southern China. Several microRNAs (miRNAs) have been shown to have an inhibitive effect on NPC, while the effect of miR-15a on NPC remains unclear. Thus, our study aimed to investigate the potential effect of miR-15a on NPC cell proliferation, apoptosis, and possible functional mechanism. Human NPC CNE1 cells were transfected with miR-15a mimics, miR-15a inhibitors, or a control. Afterward, cell viability and apoptosis were assayed by using CCK-8, BrdU assay, and flow cytometry. Moreover, Western blot was used to detect the… More >