YaJie Cui^*†, Kai’e She^‡, Defu Tian^§, Peilian Zhang^†, Xiaoyan Xin^*

Oncology Research, Vol.23, No.6, pp. 275-282, 2015, DOI:10.3727/096504016X14562725373798

Abstract Epithelial ovarian cancer (EOC) is the most lethal gynecological malignancy, accounting for 90% of all ovarian cancer. Dysregulation of miRNAs is associated with several types of EOC. In the current research, we aimed to study the role of abnormal expression of miR-146a in the development of EOC and to elucidate the possible molecular mechanisms. Compared with control samples, mRNA expression of miR-146a was significantly decreased in EOC tissues and cell lines. Overexpression of miR-146a prohibited cell proliferation, enhanced apoptosis, and increased sensitivity to chemotherapy drugs in EOC cells. In contrast, downregulation of miR-146a promoted cell… More >

Meng Lu¹, Yi Miao¹, Lan Qi, Mingzhu Bai, Jiarong Zhang, Youji Feng

Oncology Research, Vol.23, No.6, pp. 267-274, 2015, DOI:10.3727/096504016X14549667333963

Abstract FKBP14 belongs to the family of FK506-binding proteins (FKBPs). Altered expression of FKBPs has been reported in several malignancies. This study aimed to reveal the expression profile of FKBP14 in ovarian cancer and evaluate whether FKBP14 is a molecular target for cancer therapy. We found that the FKBP14 mRNA level was significantly higher in ovarian cancer tissues than in normal tissues. FKBP14 expression was then knocked down in two ovarian cancer cell lines, SKOV3 and HO8910 cells, by a lentiviral short hairpin RNA (shRNA) delivery system. Reduced expression of FKBP14 markedly impaired the proliferative ability More >

Benjamin Piura^1,4, Liat Medina^2,4, Alex Rabinovich^1,4, Victor Dyomin^3,4, Mahmoud Huleihel^2,4

European Cytokine Network, Vol.24, No.3, pp. 122-129, 2013, DOI:10.1684/ecn.2013.0342

Abstract Background. Thalidomide inhibits TNF-α production in lipopolysaccharide-stimulated monocytes. The aim of this study was to evaluate the effect of thalidomide on TNF-α, IL-6 and MMP secretion in epithelial ovarian carcinoma cells. Materials and methods. SKOV-3 cells and primary epithelial ovarian carcinoma cells were cultured in the presence of various concentrations of thalidomide. Cell proliferation was examined by MTT proliferation assay. TNF-α and IL-6 levels were determined in the supernatants of the cell cultures by ELISA, and MMP activity was examined by gelatin zymography. Results. Thalidomide did not significantly affect the proliferation and growth of SKOV-3… More >

European Cytokine Network, Vol.24, No.3, pp. 106-113, 2013, DOI:10.1684/ecn.2013.0340

Abstract In the present study, associations between pretreatment interleukin 6 (IL-6), interleukin 8 (IL-8) and C-reactive protein (CRP) serum levels and epithelial ovarian cancer (EOC) were analyzed using commercially available, enzyme-linked immunosorbent assay (ELISA) in 118 patients and 64 control subjects. Values were correlated with clinicopathological characteristics and outcomes. Control variables included age, stage, grade, histological type and residual tumor size. Kaplan-Meier plots and univariate and multivariate Cox proportional hazards models were used to study the associations between IL-6, IL-8 and CRP levels, control variables, overall survival and disease-free survival.The median IL-6, IL-8 and CRP serum… More >

Bozena Dobrzycka¹, Slawomir J. Terlikowski¹, Oksana Kowalczuk², Maciej Kinalski³

European Cytokine Network, Vol.20, No.3, pp. 131-134, 2009, DOI:10.1684/ecn.2009.0161

Abstract The significance of circulating levels of TNF-α and its soluble receptors (sTNF-Rs) in the plasma of patients with epithelial ovarian cancer (EOC) has not been fully elucidated. The present study was to investi-gate the relationship of pretreatment plasma levels of TNF-α, sTNFR-1 and sTNFR-2 with outcome in 126 patients with EOC. Concentrations of TNF-α and sTNF-Rs were determined by enzyme-linked immunosor-bent assay (ELISA). Median TNF-α and sTNF-Rs levels were significantly higher in EOC patients than in healthy controls. High plasma levels of TNF-α and sTNF-Rs were correlated with tumor stage and with reduced mean survival More >

Harika Atmaca¹, Gurbuz Gorumlu², Burcak Karaca², Mustafa Degirmenci², Didem Tunali², Yalcin Cirak², Duygu Unuvar Purcu¹, Selim Uzunoglu¹, Bulent Karabulut², Ulus A. Sanli², Ruchan Uslu²

European Cytokine Network, Vol.20, No.3, pp. 121-130, 2009, DOI:10.1684/ecn.2009.0159

Abstract In the present study, we aimed to evaluate the possible synergistic, cytotoxic effects of combination treatment of gossypol and zoledronic acid, in human ovarian cancer cell lines, OVCAR-3 and MDAH-2774, and to elucidate the role of this novel combination treatment on angiogenesis-related molecules in ovarian cancer. The XTT cell viability assay was used for showing cytotoxicity. Both DNA fragmentation by ELISA assay and caspase 3/7 activity measurement were used for demonstrating apoptosis. To elucidate the angiogenic molecules affected by combination treatment, mRNA levels of angiogenic molecules were measured using the Human Angiogenesis RT² Profiler^TM PCR Array… More >