IIZUKA YOKO¹, SAMIN HONG², CHAN YUN KIM², SEUNG KAB KIM², GONG JE SEONG²

BIOCELL, Vol.32, No.3, pp. 245-250, 2008, DOI:10.32604/biocell.2008.32.245

Abstract Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 μM) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal More >

WALTER MANUCHA

BIOCELL, Vol.31, No.1, pp. 1-12, 2007, DOI:10.32604/biocell.2007.31.001

Abstract Congenital obstructive nephropathy is the primary cause of end-stage renal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstructive uropathy effects -decline in the plasmatic renal flow and glomerular filtration rate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosis- are a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-β (TGF-β) and… More >

Galatro Andrea, Ivan Rousseau, Susana Puntarulo

Phyton-International Journal of Experimental Botany, Vol.75, pp. 7-20, 2006, DOI:10.32604/phyton.2006.75.007

Abstract Electron Paramagnetic Resonance (EPR) has been developed as a versatile field that uses different techniques sharing the common feature of resonant micro-wave radiation absorption by paramagnetic substances. Certain free radicals, such as ascorbyl radical (A•), show an stable spectrum, and can be directly detected by EPR at room temperature. Studies included in this work clearly show that this highly sensitive methodology can be successfully applied to biological systems. Exposure of plants to UV-B radiation was studied as a factor of oxidative stress in plants, and the effect on A^• steady state concentration was analyzed. A kinetic More >

G.C. DALVIT, P.D. CETICA, L.N. PINTOS, M.T. BECONI

BIOCELL, Vol.29, No.2, pp. 209-212, 2005, DOI:10.32604/biocell.2005.29.209

Abstract Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO₂: 95% humidified air. In vitro fertilization was carried out in… More >