Nidia FERRAROTTI¹, Rosario MUSACCO-SEBIO², Christian SAPORITO-MAGRIÑÁ², Juan Manuel ACOSTA², Marisa REPETTO^{2 *}

BIOCELL, Vol.40, No.1, pp. 19-22, 2016, DOI:10.32604/biocell.2016.40.019

Abstract The objective of this work was to study the in vivo time course of biochemical processes of oxidative damage in the brain of Sprague-Dawley rats that received an acute overload of the redox active metals iron (Fe) and copper (Cu), and the redox inactive cobalt (Co) and nickel (Ni). Oxidative stress indicators (phospholipid and protein oxidation), glutathione (GSH), antioxidant enzymes and NADPH oxidase activities, and the plasma inflammatory cytokine (IL-6) were measured. The results showed that in brain oxidative mechanisms for both sets of metal are different, however in both cases are irreversible. The mechanism… More >

Jimena P. CABILLA, Sonia A. RONCHETTI, Beatriz H. DUVILANSKI^*

BIOCELL, Vol.40, No.1, pp. 15-18, 2016, DOI:10.32604/biocell.2016.40.015

Abstract Environmental contamination with some metalloids and heavy metals (M/HM) raises concern due to well known adverse effects on health. Among these pollutants, chromium VI (Cr VI), cadmium (Cd) and arsenic (As) are frequently present as a result of natural sources or due to industrial activities. They are able to easily enter the organism and negatively affect many organs and systems. In vivo (exposure to Cr VI, Cd or As through drinking water) and in vitro experiments (primary pituitary cell cultures) were performed in male Wistar rats to address their actions on hypothalamus-pituitary axis. All the… More >

Bustingorri C, K Balestrasse, RS Lavado

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 407-416, 2015, DOI:10.32604/phyton.2015.84.407

Abstract Arsenic (As) and Fluoride (F) are present in many soils, affecting crops and posing risks in the food chain. We performed pot experiments on spiked soils enriched in these elements either individually or simultaneously, over a wide range of concentrations. Soybean biomass production, grain yield, As and F accumulation and distribution within the plant, and the antioxidant response to these stresses were analyzed. Arsenic was more toxic than F. At As levels >35 mg/kg and F levels >375 mg/kg, yield loss reached 60% and 30%, respectively. At the highest dose of As plants died within More >

Berengere PAPEGAY^{1, *}, Michaela STADLER¹, Vincent Nuyens¹, Isabelle SALMON², Veronique KRUYS³, Jean G. BOOGAERTS¹

BIOCELL, Vol.38, No.1, pp. 25-32, 2014, DOI:10.32604/biocell.2014.38.025

Abstract Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery, ischemic states and multiple organ failure. The pre-existing nutritional status of the liver, i.e., fasting, might contribute to the extent of tissue injury. This study investigated whether alanine, an amino acid precursor of glucose, could protect ex vivo perfused livers of fasting rats from reperfusion injury. The portal vein was cannulated, the liver removed and perfused in a closed ex vivo system. Isolated livers were perfused either with glucose 1 g/L and 10 g/L, or with equal concentrations of alanine (n = 10 in… More >

VIJAY L. KUMAR^* AND BISWA M. PADHY

BIOCELL, Vol.35, No.3, pp. 63-70, 2011, DOI:10.32604/biocell.2011.35.063

Abstract Calotropis species have been used in the traditional medicinal system for the treatment of diseases of the liver and abdomen. In view of the antioxidant and anti-hyperglycemic properties of an aqueous suspension obtained from the dried latex of Calotropis procera, the present study was carried out to evaluate its efficacy in affording protection against alloxan induced changes in rat kidney. A single intraperitoneal injection of alloxan (150 mg/kg) in rats produced hyperglycemia within 3 days and altered kidney functions over a period of 90 days. Daily oral administration of the aqueous suspension (100 and 400 mg/kg) in… More >

IIZUKA YOKO¹, SAMIN HONG², CHAN YUN KIM², SEUNG KAB KIM², GONG JE SEONG²

BIOCELL, Vol.32, No.3, pp. 245-250, 2008, DOI:10.32604/biocell.2008.32.245

Abstract Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 μM) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal More >

WALTER MANUCHA

BIOCELL, Vol.31, No.1, pp. 1-12, 2007, DOI:10.32604/biocell.2007.31.001

Abstract Congenital obstructive nephropathy is the primary cause of end-stage renal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstructive uropathy effects -decline in the plasmatic renal flow and glomerular filtration rate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosis- are a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-β (TGF-β) and… More >

Galatro Andrea, Ivan Rousseau, Susana Puntarulo

Phyton-International Journal of Experimental Botany, Vol.75, pp. 7-20, 2006, DOI:10.32604/phyton.2006.75.007

Abstract Electron Paramagnetic Resonance (EPR) has been developed as a versatile field that uses different techniques sharing the common feature of resonant micro-wave radiation absorption by paramagnetic substances. Certain free radicals, such as ascorbyl radical (A•), show an stable spectrum, and can be directly detected by EPR at room temperature. Studies included in this work clearly show that this highly sensitive methodology can be successfully applied to biological systems. Exposure of plants to UV-B radiation was studied as a factor of oxidative stress in plants, and the effect on A^• steady state concentration was analyzed. A kinetic More >

G.C. DALVIT, P.D. CETICA, L.N. PINTOS, M.T. BECONI

BIOCELL, Vol.29, No.2, pp. 209-212, 2005, DOI:10.32604/biocell.2005.29.209

Abstract Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO₂: 95% humidified air. In vitro fertilization was carried out in… More >