KARINA MANCINI, HEIDI DOLDER

BIOCELL, Vol.28, No.3, pp. 299-310, 2004, DOI:10.32604/biocell.2004.28.299

Abstract This study was undertaken to detect protein components in both sperm types of the butterfly Euptoieta hegesia. These spermatozoa possess complex extracellular structures for which the composition and functional significance are still unclear. In the apyrene sperm head, the proteic cap presented an external ring and an internal dense content; basic proteins were detected only in external portions. In the tail, the paracrystalline core of mitochondrial derivatives and the axoneme are rich in proteins. The extratesticular spermatozoa are covered by a proteic coat, which presented two distinct layers. In eupyrene spermatozoa, acrosome and nucleus were negatively stained, probably because of… More >

ELIANE VICTORIANO, ELISA A. GREGÓRIO

BIOCELL, Vol.28, No.2, pp. 165-169, 2004, DOI:10.32604/biocell.2004.28.165

Abstract The Lyonet’s gland is found in Lepidoptera larvae, close to the excretory duct of the silk gland. The role played by this gland is still uncertain. This work aims to describe the ultrastructure of the Lyonet’s gland in Diatraea saccharalis larvae, offering suggestions regarding its possible function. The insects were reared under laboratory-controlled conditions. The glands were conventionally prepared for transmission (TEM) and scanning (SEM) electron microscopy. SEM showed that Lyonet’s glands are paired small structures located in the ventral side of the head. They are composed by clustered long cells resembling leaves. Under TEM observations, each cell is surrounded… More >

ADELINA FERREIRA, HEIDI DOLDER

BIOCELL, Vol.27, No.3, pp. 353-362, 2003, DOI:10.32604/biocell.2003.27.353

Abstract Spermatogenesis, with emphasis on spermiogenesis, is described for the lizard, Tropidurus itambere, using light microscopy, phase contrast and epifluorescence, as well as scanning and transmission electron microscopy. Cellular differentiation involves events of chromatin condensation, nuclear elongation and the formation of structural complexes, such as the acrosomal and axonemal ones. Other new characteristics, exclusive for this species, include various aspects of the subacrosomal granule, the insertion of the proacrosomal vesicle and the development of these structures to participate in the acrosomal complex. Radial projections occur just above the nuclear shoulders, which have been recognized already from the beginning of cellular elongation.… More >

Moya ME^1,2, AA Galussi^1,2, MJ Gómez^1,2, FG Marchese^1,2

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 176-183, 2015, DOI:10.32604/phyton.2015.84.176

Abstract The aim of the present work was to analyze the foliar anatomy in lamina transverse section an in epidermis superficial view of Mnesithea selloana. Epidermic preparations and transections were carried out of the medium leaf part of sterile innovations that were conditioned and observed with optic microscope (OM) and scanning electron microscope (SEM).The histofoliar characteristics observed were: epidermic, intercostal, subsidiary, suberose, silicea and bulliform cells; and also guard cells, hairs, waxes, mesophylls, Kranz sheath, vascular bundles, sclerenchyma, colourless parenchyma and leaf margin. Epidermic observations showed that M. selloana presents a panicoid type epidermis presenting silicic cells as halteriforme, longer than… More >

Cammaerts MC¹, O Johansson²

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 132-137, 2015, DOI:10.32604/phyton.2015.84.132

Abstract Under high levels of radiation (70-100 µW/m2 =175 mV/m), seeds of Brassicaceae Lepidium sativum (cress d’Alinois) never germinated. In fact, the first step of seeds’ germination ‒ e.g. imbibitions of germinal cells ‒ could not occur under radiation, while inside the humid compost such imbibitions occurred and roots slightly developed. When removed from the electromagnetic field, seeds germinated normally. The radiation was, thus, most likely the cause of the non-occurrence of the seeds’ imbibitions and germination. More >

Gil SP, L Seisdedos, ME Reyna, MM Cerana

Phyton-International Journal of Experimental Botany, Vol.81, pp. 205-210, 2012, DOI:10.32604/phyton.2012.81.205

Abstract The goals of this study were (1) to make a careful study of the foliar epidermis of Grindelia cabrerae var. cabrerae, Solidago chilensis y Wedelia buphthalmiflora, native Asteraceae from Argentina which have ornamental potential, and (2) to determine variables that indicate adaptation to xeromorphic environments. Five adult leaves from five plants were selected from each population. All the material was fixed in FAA. The epidermis of both foliar sides were macerated in hypochlorite of sodium, and obtained by scraping. They were stained with safranine and mounted in water-glycerine (1:1). From each slide, five fields were chosen and frequency of the… More >

Cerna¹ E, Y Ochoa², R Mendoza¹, MH Badii³, G Gallegos¹, J Landeros¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 147-152, 2010, DOI:10.32604/phyton.2010.79.147

Abstract Protein assays were conducted on Tetranychus urticae Koch, as potential resistance detection tools to plaguicides. This is a phytophagous mite that feeds on a large variety of plants. Experiments were carried out using a pesticide susceptible and three field crop strains of T. urticae. Protein was measured by colorimetric assays, using Kit-II from Bio-Rad, with bovine serum albumin (BSA) as standard. Homogenates were prepared using 10, 30, 50, 100, 300, 500 and 800 mites, with 30 replicates each. Linearity was obtained for the standard curve of the different methods, and r² values ranged from 0.877 to 0.985. The Bradford method… More >

Foroughbakhch R, RJ Ferry Sr, JL Hernández-Piñero, MA Alvarado-Vázquez, A Rocha-Estrada

Phyton-International Journal of Experimental Botany, Vol.77, pp. 113-127, 2008, DOI:10.32604/phyton.2008.77.113

Abstract Orchids of the genus Stanhopea are currently identified only by their floral structure characteristics. A statistical analysis of a significant number of species of this genus disclosed that measurements of adaxial and abaxial epidermal cell surface areas can be correlated with specific recognized species by a leaf printing method. This allows an objectively either positive or nearly positive confirmation of the identity of a species in the absence of flowers and without damage to plants. When ordering the mean values obtained for these surface areas in each species in a decreasing order, a correlation was observed in a hierarchical way… More >

Maldonado FI¹, JF Ramírez², M Rubí², X Antonio³, AV Lara⁴, AD Acosta⁴, R Rivera⁴, AL Ávila⁵

Phyton-International Journal of Experimental Botany, Vol.86, pp. 97-111, 2017, DOI:10.32604/phyton.2017.86.097

Abstract Avocado (Lauraceae: Persea americana Mill.) is a crop of great economic importance for Mexico in terms of perennial production systems, since in recent years the marketing of this fruit has increased markedly at national and international levels. In 2013, 168113.64 ha were cultivated in Mexico, with an average yield of 10.2 ton/ha, obtaining a production of approximately 1467837.35 ton. Like any other fruit can suffer damage from pests (insects, nematodes, mites etc.) and diseases (caused by fungi, bacteria and viruses). In this regard, one of the most problematic insects is rankliniella occidentalis, which causes significant crop losses, feeding preferably on… More >

Mingxing Ouyang^1,*, Zhili Qian¹, Yang Jin¹, Linhong Deng¹

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 65-65, 2019, DOI:10.32604/mcb.2019.06642

Abstract The collective functions at cell population level rely on cell-cell communications with or without direct contacts [1-3]. The long-range biomechanical force propagating across certain scales far beyond single cell size may reserve the capability to trigger coordinative biological responses within cell population [3-5]. Whether and how cells communicate with each other mechanically in a distant manner remains largely to be explored. Airway smooth muscle (ASM) cells are one crucial component in providing mechanical support and contraction force for the bronchial tubes in respiratory system, whereas the mechanical property of ASM is also associated with asthma attack, and during airway hyper-responsiveness,… More >