Zongqiang GAO¹, Xiong GUO², Junchang CHEN¹, Chen DUAN²

BIOCELL, Vol.43, No.2, pp. 99-102, 2019, DOI:10.32604/biocell.2019.06520

Abstract This study aimed to investigate the effect of hyaluronic acid (HA) on the expression of heat shock protein 70 (HSP70) in chondrocytes isolated from patients with osteoarthritis (OA) and Kashin-Beck disease (KBD). The chondrocytes were collected from OA and KBD patients, and chondrocytes isolated from patients of accident injuries were used as the control. The chondrocytes were treated with HA at different doses. HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis. Compared with control, both mRNA and protein levels of HSP70 were higher in chondrocytes from KBD and OA. However,… More >

Mahmoud MOUSTAFA^1,2, Saad ALAMRI¹, Hoida ZAKI², Naglaa LOUTFY², Tarek TAHA³, Ali SHATI¹, Mohamed ALKAHTANI¹, Sajda SIDDIQI¹

BIOCELL, Vol.43, No.2, pp. 81-88, 2019, DOI:10.32604/biocell.2019.06231

Abstract The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (Pisum sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases, cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds… More >

Sitta Aroonnual Paisan Kanthang, Wannapong Triampo¹

The International Conference on Computational & Experimental Engineering and Sciences, Vol.13, No.3, pp. 53-54, 2009, DOI:10.3970/icces.2009.013.053

Abstract How does a cell successfully divide is one of very fundamental questions in biological and medical science especially concerning mechanism. With this regards, MinE proteins are very important for Escherichia coli cell division process because it supports FtsZ proteins to form at mid-cell which lead to cell division at that region. In this work, we quantitatively studied the physical properties of MinE protein clusters including pattern formations and dynamic motions using both theoretical and experimental approach. Experimentally, through the spot tracking technique (STT) and diffusion analysis, it was found that MinE globally performed oscillatory motion from middle cell to the… More >

Somchai Sriyab¹, Wannapong Triampo²

The International Conference on Computational & Experimental Engineering and Sciences, Vol.13, No.3, pp. 51-52, 2009, DOI:10.3970/icces.2009.013.051

Abstract Understanding of Bacteria cell division is essential for an understanding of microorganism as well as the origin of the life. Particularly, in cell division process of \emph {E. coli}, Min proteins (MinD and MinE) play crucial roles to regulate the dividing dynamics physically via their oscillatory dynamics from pole to pole. In this work, we have developed a numerical scheme based on the mesoscopic Lattice Boltzmann Method (LBM) to simulate the coarse-grained coupled reaction-diffusion equations model used to describe the MinD/MinE interaction in two dimensions. Biologically, we have focused on investigating how the protein copies affect the oscillation patterns as… More >

Waipot Ngamsaad, Wannapong Triampo¹

The International Conference on Computational & Experimental Engineering and Sciences, Vol.13, No.2, pp. 49-50, 2009, DOI:10.3970/icces.2009.013.049

Abstract The Min-proteins oscillation in \emph {E. coli} has an essential role in controlling the accuracy placement of cell-division septum at the middle cell zone of the bacteria. This biochemical process has been successfully described by a set of reaction-diffusion equation at the macroscopic level [1]. Recently, a mesoscopic modeling by the lattice Boltzmann method (LBM) has been proposed to simulate the Min-proteins oscillation [2]. However, as pointed out by Zhang et al., the standard boundary conditions are not accuracy for a class of dispersion transport modeled by LBM [3]. In this present work, we investigated the boundary effects in LBM… More >

S.R.K Vedula¹, T.S. Lim², W. Hunziker³, C.T. Lim¹

The International Conference on Computational & Experimental Engineering and Sciences, Vol.13, No.1, pp. 29-30, 2009, DOI:10.3970/icces.2009.013.029

Abstract The structural integrity as well as the regulation of paracellular diffusion of solutes across epithelial monolayers is critically regulated by the intercellular adhesion complex. The intercellular adhesion complex consists of a variety of proteins that perform different physiological functions. While proteins localizing at adherens junctions (nectins and e-cadherins) are important for initiating and stabilizing cell adhesion, proteins localizing at the tight junctions (occludin, claudins and junctional adhesion molecules) act as gates to regulate the diffusion of solutes across the epithelial monolayer. Despite significant advancement in the understanding of the biological roles of these cell adhesion proteins in regulating various cellular… More >

Qingjia Chi*, Xinge Geng

The International Conference on Computational & Experimental Engineering and Sciences, Vol.22, No.1, pp. 118-118, 2019, DOI:10.32604/icces.2019.05168

Abstract The binding of DNA to protein in the cellular nucleus is a common phenomenon. DNA molecules will soften at the binding region when they adhere to proteins. Softening will affect the mechanical properties significantly. However, the mechanism underlying the mechanical softening remains to be explored. To understand the changes in the mechanical properties of DNA, the peridynamics technique can effectively capture the stress of the softened DNA under tensile forces. And later the results were verified by finite element computations. Utilizing the computations of perydynamics to reveal the stretch of the double-stranded DNA. The results demonstrated DNA was easy to… More >

Rakesh Kumar^1,*, Reshma Praveen¹, Shikha Rani¹, K. Sharma², K. P. Tiwary^3,*, K. Dinesh Kumar⁴

Journal of Renewable Materials, Vol.7, No.8, pp. 749-761, 2019, DOI:10.32604/jrm.2019.06286

Abstract ZnSe nanoparticles have been synthesized by microwave assisted method by using zinc chloride, selenium powder and ethylene diamine. The synthesized nanoparticles have been characterized structurally by FT-IR and XRD as well as morphological characterization was done by scanning electron microscope (SEM). The crystallite size after synthesis was obtained around 30 nm for pure ZnSe nanocrystallites. However, from SEM micrograph, agglomerated ZnSe nanoparticles of irregular shapes were observed. The as-synthesized ZnSe nanoparticles at different contents (1 to 5% w/w w.r.t SPI) were incorporated into soy protein isolate (SPI) to produce reinforced SPI films by solution casting method. The ZnSe nanoparticles incorporated… More >

Rakesh Kumar^1,*, Priya Rani¹, K. Dinesh Kumar²

Journal of Renewable Materials, Vol.7, No.2, pp. 103-115, 2019, DOI:10.32604/jrm.2019.00027

Abstract Soy protein isolate (SPI) biopolymeric films were prepared by adding different contents of mandelic acid (1 to 5% wrt SPI) to glycerol plasticized SPI by solution casting method. Also, SPI was fermented by Bacillus subtilis to get fermented SPI films by solution casting. Molecular mass determination of mandelic acid incorporated and fermented SPI films was carried out by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Mandelic acid incorporated and fermented SPI films were characterized by Fourier-transform infrared spectroscopy (FT-IR), dynamic mechanical analysis (DMA), tensile strength, water uptake and optical transmittance studies. Results indicated that incorporation of mandelic acid in SPI resulted… More >

G. Yoon¹, K. Bong², J. Kim³, I.H. Ahn⁴, K. Eom⁵, S. Na⁶

The International Conference on Computational & Experimental Engineering and Sciences, Vol.2, No.2, pp. 53-60, 2007, DOI:10.3970/icces.2007.002.053

Abstract This paper presents multi-component mode methodology applicable to biomolecular structures for understanding the dynamics of proteins. Even though the conventional normal mode analysis has been contributed for analyzing the dynamics and thermal fluctuations of proteins, it frequently encounters with the computational prohibition for large proteins due to memory requirement. To overcome the conventional computational limitations, the drawback motivates one to develop various model reduction methods, which reduces the degrees of freedom of the full model so as to decrease the computational expense, while the computational accuracy is maintained. Our results demonstrate that the multi-component modal analysis applied to the biomolecular… More >