S.K. VILA*, H.Y. REY, L.A. MROGINSKI

BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l^-1 benzylaminopurine (BAP) + 0.1mg.l^-1 kinetin (KIN) + 3 mg.l^-1 adenine sulphate (ADS).
The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the More >

MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO

BIOCELL, Vol.26, No.2, pp. 263-266, 2002, DOI:10.32604/biocell.2002.26.263

Abstract The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of More >

L.A. CARO¹, P.A. POLCI¹ , L.I. LINDSTRÖM¹ , C.V. ECHENIQUE^1,2, L.F. HERNÁNDEZ^1,3

BIOCELL, Vol.26, No.1, pp. 25-33, 2002, DOI:10.32604/biocell.2002.26.025

Abstract Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 – 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm,… More >