N.M. OCARINO*, A. BOZZI**, R.D.O. PEREIRA*, N.M. BREYNER**, V.L. SILVA*, P. CASTANHEIRA**, A.M. GOES**, R. SERAKIDES*

BIOCELL, Vol.32, No.2, pp. 175-183, 2008, DOI:10.32604/biocell.2008.32.175

Abstract 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long… More >

FRANCISCO CAPANI¹, EZEQUIEL SARACENO¹, VALERIA ROMINA BOTI¹, LAURA AON-BERTOLINO¹, JUAN CARLOS FERNÁNDEZ¹, FERNANDO GATO¹, MARIA SOL KRAUSE², LISANDRO GIRALDEZ³, MARK H. ELLISMAN⁴, HÉCTOR COIRINI^1,2

BIOCELL, Vol.32, No.1, pp. 1-8, 2008, DOI:10.32604/biocell.2008.32.001

Abstract Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining More >

Pincheira-Ulbrich¹ J, JR Rau², E Hauenstein³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 321-326, 2008, DOI:10.32604/phyton.2008.77.321

Abstract We sampled the diversity of trees and shrubs, and built the rarefaction and accumulation species curve in 10 native forest fragments in southern Chile. Results indicated that: (1) there were 23 species in all sampled fragments, (2) species richness increased significantly with fragment size, (3) all fragments require a minimal sampling from 40 to 170 individuals to reach the maximum theoretical species richness, and (4) sampling richness was greater than that theoretically expected (20 species). More >

Sacristán M, AM Millanes, B Fontaniella, ME Legaz, C Vicente

Phyton-International Journal of Experimental Botany, Vol.77, pp. 253-262, 2008, DOI:10.32604/phyton.2008.77.253

Abstract Secreted arginases from Evernia prunastri and Xanthoria parietina thalli, exhibiting lectin activity, have been purified to homogeneity. Analyses of both amino acid and glycoside composition are reported. Purified proteins have been subjected to tryptic digestion and some amino acid sequences have been analyzed. An undecapeptide from Evernia arginase, which shows some degree of homology with the domain to which Mn²⁺ binds, a heptapeptide and an undecapeptide from Xanthoria arginase have been analyzed, and homologies with some arginases from other fungi and plants have been found. More >

A.B. Mahmud Hasan^1,2, M A Wahab¹, S.M. Guo¹

Structural Durability & Health Monitoring, Vol.4, No.4, pp. 231-240, 2008, DOI:10.3970/sdhm.2008.004.231

Abstract This paper presents details analysis of Heat Affected Zone (HAZ) in High Temperature Vacuum Tube Furnace (HTVTF) to predict fracture region in tube wall due to thermal stress from temperature variation across its thickness. A simple mathematical model is used to illustrate stress concentration arising due to thermal stresses in tube wall and HAZs. Finite element model and analysis were carried out utilizing finite element analysis commercial codes. Combination of thermal stress due to temperature difference determine fracture region. Experimental and simulation results of inside temperature of tube are observed and similar relation have been More >

B.K.Raghuprasad¹, N.Lakshmanan², N.Gopalakrishnan², K.Muthumani²

Structural Durability & Health Monitoring, Vol.4, No.3, pp. 117-144, 2008, DOI:10.3970/sdhm.2008.004.117

Abstract The reduction in natural frequencies, however small, of a civil engineering structure, is the first and the easiest method of estimating its impending damage. As a first level screening for health-monitoring, information on the frequency reduction of a few fundamental modes can be used to estimate the positions and the magnitude of damage in a smeared fashion. The paper presents the Eigen value sensitivity equations, derived from first-order perturbation technique, for typical infra-structural systems like a simply supported bridge girder, modelled as a beam, an end-bearing pile, modelled as an axial rod and a simply… More >

L. H. Feng¹, J. H. Feng¹

The International Conference on Computational & Experimental Engineering and Sciences, Vol.5, No.1, pp. 15-20, 2008, DOI:10.3970/icces.2008.005.015

Abstract The primary scheme of unilaterally pursuing fast economic development at the expense of the environment and the secondary scheme focusing on environmental protection as the primary goal by slowing economic development are both undesirable for Yiwu. The third scheme balancing economic development with environmental protection is the most effective scheme. More >

Young-Sook Roh¹, Lan Chung², Seung-Ho Cho², Se-Hyun Yoon², Jung-Tae Noh

The International Conference on Computational & Experimental Engineering and Sciences, Vol.8, No.4, pp. 157-164, 2008, DOI:10.3970/icces.2008.008.157

Abstract The purpose of this research is to develop a new technique to measure the corrosion level of reinforcing rebar which embedded in reinforced concrete structures using infrared(IR) thermographical image. IR thermography has been used various applications for non-destructive test(NDT) area, however, IR thermographical data depend on many parameters that the assessment of reliability and stability becomes very important issue to utilize IR thermographical system. This study presents a technique to quantitatively measure the corrosion level of a reinforcing bar using proposed infrared thermography measurement system. We found out electric heating method has an important effect More >

Q. Ma^1,2, J. L. Beuth¹, G. H. Meier³, F. S. Pettit³

The International Conference on Computational & Experimental Engineering and Sciences, Vol.8, No.1, pp. 13-18, 2008, DOI:10.3970/icces.2008.008.013

Abstract This paper addresses the mechanics of indentation-induced delamination of a coating on a multi-layered hollow cylinder, for application to toughness testing of thermal barrier coating (TBC) systems. An electron-beam physical vapor deposition (EB-PVD) TBC system has been analyzed through a contact finite element model using a standard conical indenter impressed vertically on the top of the cylinder. Two cylinder sizes used in thermal-mechanical fatigue tests in literature have been considered in the analysis, with outer radii of 5.11 mm and 3.08 mm, and inner radii of 3.00 mm and 0.97 mm, respectively. Two boundary conditions More >

G. Kanevce¹, L. Kanevce², V. Mitrevski³, G. Dulikravich⁴

The International Conference on Computational & Experimental Engineering and Sciences, Vol.8, No.1, pp. 1-6, 2008, DOI:10.3970/icces.2008.008.001

Abstract This paper deals with the application of inverse concepts in drying. The objective of the paper is estimation of the moisture diffusivity of a drying body by using only temperature measurements. Potato and apple slices have been chosen as representative drying bodies with significant shrinkage effects. A mathematical model of the drying process of shrinking bodies has been applied. The Levenberg-Marquardt method and a hybrid optimization method of minimization of the least-squares norm were used to solve the present parameter estimation problem. More >