KAN OUYANG^1,2,#, MEIQUAN XU^3,#, YUJIE LIANG⁴, XIAO XU², LIMEI XU², CAINING WEN^1,2, ZHUAN QIN², YIXIN XIE², HUAWEI ZHANG⁵, LI DUAN^2,*, DAPING WANG^1,2,5,*

BIOCELL, Vol.46, No.6, pp. 1521-1526, 2022, DOI:10.32604/biocell.2022.018788 - 07 February 2022

Abstract There is no efficient tracking system available for the therapeutic molecules delivered to cartilage. The dense matrix covering the cartilage surface is the main biological barrier that the therapeutic molecules must overcome. In this study, we aimed to establish a system that can dynamically and effectively track the therapeutic molecules delivered to cartilage. To this aim, we adopted bovine and human cartilage explants as ex vivo models for chondrocyte-targeted exosome dispersion. The efficiency of drug delivery was evaluated using frozen sections. The results of this study showed that the penetration and distribution of chondrocyte-targeted exosomes in More >

YAO XU^1,#, BIN WU^2,#, JING YANG³, SHENG ZHANG², LONGGEN LIU⁴, SUOBAO XU^2,*, JIAKAI JIANG^2,*

BIOCELL, Vol.46, No.6, pp. 1483-1491, 2022, DOI:10.32604/biocell.2022.017745 - 07 February 2022

Abstract To study the roles and association of murine double minute gene 2 (MDM2) and fructose-1,6-biphosphatase (FBP1) in human hepatocellular carcinoma (HCC), growth response of human HCC cells was assessed using proliferation and apoptosis assay. Pro-survival AKT signaling associated proteins (p-AKT, survivin and cleaved caspase 3) were assessed using western blotting. The correlation between MDM2 and FBP1 was assessed using co-immunoprecipitation combined with ubiquitination assay. Our data suggested that low expression of FBP1 was correlated with high levels of MDM2 in HCC cell lines (Huh7 and Hep3B). Overexpression of FBP1 resulted in anti-proliferation, pro-apoptosis, the up-regulation… More >

Chengfan Li^1,2, Lan Liu^3,*, Junjuan Zhao¹, Xuefeng Liu⁴

CMES-Computer Modeling in Engineering & Sciences, Vol.131, No.1, pp. 429-444, 2022, DOI:10.32604/cmes.2022.019202 - 24 January 2022

Abstract Target detection of small samples with a complex background is always difficult in the classification of remote sensing images. We propose a new small sample target detection method combining local features and a convolutional neural network (LF-CNN) with the aim of detecting small numbers of unevenly distributed ground object targets in remote sensing images. The k-nearest neighbor method is used to construct the local neighborhood of each point and the local neighborhoods of the features are extracted one by one from the convolution layer. All the local features are aggregated by maximum pooling to obtain global… More >

Kailei Wang, Mingjing Li^*, Peng Yan, Leiting Dong^*

CMES-Computer Modeling in Engineering & Sciences, Vol.131, No.1, pp. 165-197, 2022, DOI:10.32604/cmes.2022.019188 - 24 January 2022

Abstract The main goal of this work is to study the ballistic performance of multi-layered moderately-thick metallic targets. Several target configurations have been considered in this work, with various types of interlayer connection (spaced, contacted and adhesive) and the number of layers (four and eight), and the influence of target configurations on ballistic performance has been studied experimentally and numerically. In the experiments, the targets were impacted by 12.7-mm projectiles at a velocity around 820 m/s. The experimental results show that, with similar total thickness, the contacted and adhesive targets exhibit better ballistic performance than the monolithic… More >

RUI XU¹, QIBIAO WU¹, YUHAN GONG², YONGZHE WU¹, QINGJIA CHI^1,*, DA SUN^3,*

BIOCELL, Vol.46, No.5, pp. 1261-1288, 2022, DOI:10.32604/biocell.2022.018427 - 06 January 2022

Abstract There is currently no effective solution to the problem of poor prognosis and recurrence of HCC. The technology of immunotherapy and prognosis of genetic material has made continuous progress in recent years. In the study, a 5-gene signature was established for the prognosis of HCC through biological information, and the immune infiltration of HCC patients was studied. After studied HCC patients’ immune infiltration, the paper screened the differential target genes of miR-126-3p in HCC downloaded from TCGA database, and uses WGCNA method to select the modular genes highly relevant to M2 macrophage. Then we use… More >

YUHUA ZOU^1,#, LEI ZHANG^2,#, XIN ZHONG^3,*

BIOCELL, Vol.46, No.5, pp. 1309-1317, 2022, DOI:10.32604/biocell.2022.018167 - 06 January 2022

Abstract Renal cell carcinoma (RCC) has a poor prognosis due to limited diagnosis and treatment. Thus, it is necessary to find novel prognostic biomarkers and therapeutic targets. The aberrant expression of microRNAs plays an important role in RCC oncogenesis. Tissue inhibitors of metalloproteinase 3 (TIMP3) acts as a downstream target of miR-181b. The aim of this study was to understand the role and molecular mechanism of miR-181b in RCC oncogenesis. The results showed that miR-181b expression was significantly higher in RCC tumour tissues, especially in those with significant invasion or metastasis. miR-181b overexpression promoted proliferation and… More >

YUANNA DU^1,#, WENWEN GONG^2,#, JING LIANG^1,#, RUKUN ZANG¹, JUNJUN MOU¹

BIOCELL, Vol.46, No.5, pp. 1181-1188, 2022, DOI:10.32604/biocell.2022.016148 - 06 January 2022

Abstract Endometrial cancer remains to be a major type of malignancy in threatening female life. Molecular insights in advancing our understanding of endometrial tumorigenesis are much needed. We here report that a less-studied protein Dihydropyrimidinase like 3 (DPYSL3) is a potent tumor suppressor. DPYSL3 is uniquely regulated by wild type p53 (wtp53), and its expression is at the highest level when cells carry wtp53 and are exposed to hypoxia. We reveal that wtp53 can bind DPYSL3 promoter to enhance DPYSL3 expression and in turn, the elevated DPYSL3 can restrain cancer cell proliferation and invasion in vitro and in… More >

YANG YANG, LIJUAN FU, CHUNMEI CHEN, MEIWEI HU

BIOCELL, Vol.46, No.5, pp. 1319-1328, 2022, DOI:10.32604/biocell.2022.015760 - 06 January 2022

Abstract Aberrant expression of miRNAs is significantly correlated with the occurrence of immune thrombocytopenic purpura (ITP). The immune imbalance of M1/M2 macrophage contributes to the development of ITP. However, the role of miR-148b-3p in macrophage phenotype imbalance remains unknown in ITP. In this study, we aimed to explore whether miR-148b-3p inhibits M2 macrophage polarization in ITP and to investigate the underlying mechanism. Peripheral blood from 22 ITP patients were collected, and real-time PCR confirmed that miR-148b-3p was up-regulated and Western blot analyses detected the expression of SOCS3 was down-regulated. Subsequent dual-luciferase reporter gene assay indicated that More >

HUI-YING JIN^1,2, MAO-SONG PEI^1,2, DA-LONG GUO^1,2,*

BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718 - 15 December 2021

Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, More >

Muhammad Zafar^1,#,*, Muhammad Saif Ullah^1,#, Tareq Manzoor², Muddassir Ali³, Kashif Nazar⁴, Shaukat Iqbal⁵, Habib Ullah Manzoor⁶, Rizwan Haider¹, Woo Young Kim^7,*

CMES-Computer Modeling in Engineering & Sciences, Vol.130, No.2, pp. 723-749, 2022, DOI:10.32604/cmes.2022.017257 - 13 December 2021

Abstract In recent years, the emergence of nanotechnology experienced incredible development in the field of medical sciences. During the past decade, investigating the characteristics of nanoparticles during fluid flow has been one of the intriguing issues. Nanoparticle distribution and uniformity have emerged as substantial criteria in both medical and engineering applications. Adverse effects of chemotherapy on healthy tissues are known to be a significant concern during cancer therapy. A novel treatment method of magnetic drug targeting (MDT) has emerged as a promising topical cancer treatment along with some attractive advantages of improving efficacy, fewer side effects,… More >