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  • Open Access

    ARTICLE

    Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata

    QICHAO WANG1, WUJING ZENG1, BASHARAT ALI2, XUEMIN ZHANG3, LING XU1,*, ZONGSUO LIANG1,*

    BIOCELL, Vol.45, No.4, pp. 1107-1119, 2021, DOI:10.32604/biocell.2021.015282

    Abstract Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A. paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From… More >

  • Open Access

    ARTICLE

    Genome-wide Analysis of a Plant AT-rich Sequence and Zinc-binding Protein (PLATZ) in Triticum Aestivum

    Xiaohang He1, Minjie Liu2, Zhengwu Fang1, Dongfang Ma1,2,3,*, Yilin Zhou1,3,*, Junliang Yin1

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 971-986, 2021, DOI:10.32604/phyton.2021.012726

    Abstract Plant AT-rich sequence and zinc-binding protein (PLATZ) is a plant transcription factor that has been studied in corn. PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression. It is involved in the regulation of dehydration tolerance in seeds. In this study, we performed bioinformatics analysis to identify and characterize wheat PLATZ(TaPLATZ)genes. We identified 49 wheat PLATZ genes by searching the wheat genome by using known PLATZ gene sequences from rice, Arabidopsis, and maize. Phylogenetic analysis on PLATZ gene sequences from different species was performed. We found that PLATZs could be divided into three groups. The… More >

  • Open Access

    ARTICLE

    Potassium-Induced Regulation of Cellular Antioxidant Defense and Improvement of Physiological Processes in Wheat under Water Deficit Condition

    Abdul Awal Chowdhury Masud1, Md. Fazlul Karim1, M. H. M. Borhannuddin Bhuyan2, Jubayer Al Mahmud3, Kamrun Nahar4, Masayuki Fujita5, Mirza Hasanuzzaman1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 353-372, 2021, DOI:10.32604/phyton.2021.013259

    Abstract Drought is the most common form of abiotic stress that reduces plant growth and productivity. It causes plant injuries through elevated production of reactive oxygen species (ROS). Potassium (K) is a vital plant nutrient that notably ameliorates the detrimental effect of drought stress in the plant. A pot experiment was conducted at the Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Japan, under controlled environment of green house to explore the role of K in mitigating drought severity in wheat (Triticum asevitum L.) seedlings. Three days after germination, seedlings were exposed to three water regimes viz., 100, 50,… More >

  • Open Access

    ARTICLE

    Identification and analysis of AP2/ERF gene family in tomato under abiotic stress

    XIAOLIN ZHU1,3, XIAOHONG WEI1,3,*, BAOQIANG WANG2,3, XIAN WANG2, MINGJUN ZHANG4

    BIOCELL, Vol.44, No.4, pp. 777-803, 2020, DOI:10.32604/biocell.2020.010153

    Abstract AP2/ERE-type transcription factors, as a type of plant-specific transcription factors, play a key role in plant biotic and abiotic stress. Meanwhile, they have been studied in many plants, but rarely in tomatoes. In this study, we performed a genome-wide analysis of the SlAP2/ERF gene family of tomato, and finally identified 29 SlAP2/ERF genes and divided them into different subfamilies. At the same time, its basic physical and chemical properties were analyzed. We also constructed phylogenetic trees with 30 Arabidopsis AP2/ERF proteins and 28 potatoes AP2/ERF proteins to ensure conservative homology between them. In addition, we mapped 29 SlAP2/ERF transcription factors… More >

  • Open Access

    REVIEW

    Development of anthracnose disease resistance and heat tolerance chili through conventional breeding and molecular approaches: a review

    MST FARHANA NAZNEEN CHOWDHURY1, MOHD RAFII YUSOP1,2,*, SITI IZERA ISMAIL3, SHAIRUL IZAN RAMLEE1, YUSUFF OLADOSU2, MONIR HOSEN2, GOUS MIAH4

    BIOCELL, Vol.44, No.3, pp. 269-278, 2020, DOI:10.32604/biocell.2020.09627

    Abstract Chili (Capsicum annuum L.) is the popular spicy vegetable crops belonging to family Solanaceae. Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot or sweet pepper. Chili is used as spices, folk remedies for diseases, vegetables, and coloring agent showing a diverse role in human’s life. However, its production is hampered by different biotic stress and abiotic factors. Similarly, the unavailability of high yielding varieties, high temperature, and disease incidence, particularly, anthracnose disease, are the major constraints responsible for the low production of chili pepper. The advents of molecular markers,… More >

  • Open Access

    ARTICLE

    Reference Gene Selection for Quantitative Real-Time PCR Analyses of Acer palmatum under Abiotic Stress

    Lu Zhu, Qiuyue Ma, Shushun Li, Jing Wen, Kunyuan Yan, Qianzhong Li*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 385-403, 2020, DOI:10.32604/phyton.2020.09259

    Abstract Quantitative real-time reverse transcriptase PCR (qRT-PCR) technology has been extensively used to estimate gene expression levels, and the selection of appropriate reference genes for qRT-PCR analysis is critically important for obtaining authentic normalized data. Acer palmatum is an important colorful leaf ornamental tree species, and reference genes suitable for normalization of the qRT-PCR data obtained from this species have not been investigated. In this study, the expression stability of ten candidate reference genes, namely, Actin3, Actin6, Actin9, EF1α, PP2A, SAMDC, TIP41, TUBα, TUBβ and UBQ10, in two distinct tissues (leaves and roots) of A. palmatum under four different abiotic stress… More >

  • Open Access

    REVIEW

    Role of Pathogen-Related Protein 10 (PR 10) under Abiotic and Biotic Stresses in Plants

    Rakesh Kumar Sinha1,*, Shiv Shankar Verma2, Anshu Rastogi3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 167-182, 2020, DOI:10.32604/phyton.2020.09359

    Abstract Members of the Pathogenesis Related (PR) 10 protein family have been identified in a variety of plant species and a wide range of functions ranging from defense to growth and development has been attributed to them. PR10 protein possesses ribonuclease (RNase) activity, interacts with phytohormones, involved in hormone-mediated signalling, afforded protection against various phytopathogenic fungi, bacteria, and viruses particularly in response to biotic and abiotic stresses. The resistance mechanism of PR10 protein may include activation of defense signalling pathways through possible interacting proteins involved in mediating responses to pathogens, degradation of RNA of the invading pathogens. Moreover, several morphological changes… More >

  • Open Access

    ARTICLE

    Salinity induced anatomical and morphological changes in Chloris gayana Kunth roots

    GABRIEL CÉCCOLI*1, JULIO C. RAMOS1, LEANDRO I. ORTEGA2 , JUAN M. ACOSTA1, MARIEL G. PERRETA1

    BIOCELL, Vol.35, No.1, pp. 9-17, 2011, DOI:10.32604/biocell.2011.35.009

    Abstract Chloris gayana Kunth is a grass species valuable as forage which was introduced into Argentina to be used as pasture in saline soils of subtropical and warm-temperate zones, given its good adaptability to drought, salinity and mild freezing. However, its tolerance varies according to the cultivar. In tetraploid cultivars, important reductions in yield have been observed. Here, a study of the variations produced on the root and stem system by salinity at different NaCl concentrations (0, 150 y 250 mM) was performed in the Boma cultivar, with the aim of determining the anatomical and morphological alterations produced by the salt… More >

  • Open Access

    ARTICLE

    The use of sunflower transcription factors as biotechnological tools to improve yield and stress tolerance in crops

    Chan RL

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 5-10, 2009, DOI:10.32604/phyton.2009.78.005

    Abstract Transcription factors (TFs) are proteins able to specifically recognize DNA sequences in the regulatory regions of their target genes. They bind these specific sequences, an event that leads to the activation or repression of whole signal transduction pathways. In plants about 1500 TFs were informatically identified; identification was mainly based in the presence of DNA-binding domains in the translated sequences. They were classified in families and subfamilies according to several features, including the conservation of the DNA binding domain, the genes structures and the functions they exert. Among transcription factors, several seem to be potential powerful biotechnological tools to improve… More >

  • Open Access

    ARTICLE

    Identification of phytohormone changes and its related genes under abiotic stresses in transgenic rice

    Mohammad UBAIDILLAH1,2, Miswar FAPERTA1,2, Kyung-Min KIM1

    BIOCELL, Vol.43, No.3, pp. 215-224, 2019, DOI:10.32604/biocell.2019.07549

    Abstract Abiotic stresses, such as drought and salinity, adversely affect plant growth and productivity. Comparison between non transgenic and transgenic rice harboring CaMsrB2 gene, which induces tolerance to abiotic stress, is important to observe response of gene under abiotic stress. Phytohormone showed a tendency to increase under the drought stress or salinity stress in the transgenic plant. RT-PCR analysis showed that gene expression and phytohormone levels under abiotic stress, to be closely related. The CaMsrB2 gene is related to the expression of JA and ABA hormones. Therefore, the level of expression of these genes and hormones was observed. The transcription levels… More >

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