R. Kavitha^1,*, N. Viswanathan²

Intelligent Automation & Soft Computing, Vol.33, No.2, pp. 1305-1320, 2022, DOI:10.32604/iasc.2022.022816 - 08 February 2022

Abstract Bone marrow cancer is considered to be the most complex and dangerous disease which results due to an uncontrolled growth of white blood cells called leukocytes. Acute Lymphoblastic Leukemia (ALL) and Multiple Myeloma (MM) are considered to be important categories of bone cancers, which induces a larger number of cancer cells in the bone marrow, results in preventing the production of healthy blood cells. The advent of Artificial Intelligence, especially machine and deep learning, has expanded humanity’s capacity to analyze and detect these increasingly complex diseases. But, accurate detection of cancer cells and reducing the… More >

MIN TANG^1,#, XUELING HE^1,2,#, XINGHONG YAO¹, JIRUI WEN¹, MINGYUE BAO¹, LIANG LI^1,*

BIOCELL, Vol.46, No.6, pp. 1465-1472, 2022, DOI:10.32604/biocell.2022.018967 - 07 February 2022

Abstract The present study was designed to investigate the role of estrogen receptor α (ERα) in biaxial tensile strain (BTS) regulated osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs). rBMSCs were derived from rats and overexpressed ERα. The rBMSCs were subjected to BTS at 1 Hz with a strain of 2% for 4 h per day, 3 days, with or without ERα inhibitor ICI 182,780 (ICI). Then, bone mineralization was performed by Alizarin Red Staining. The markers of osteogenic differentiation and downstream Wnt3a/β-catenin signaling were detected by western blotting. Results showed that BTS enhanced More >

YUKI MATSUSHITA, WANIDA ONO, NORIAKI ONO^*

BIOCELL, Vol.46, No.5, pp. 1157-1162, 2022, DOI:10.32604/biocell.2022.018960 - 06 January 2022

Abstract Single-cell sequencing technologies have rapidly progressed in recent years, and been applied to characterize stem cells in a number of organs. Somatic (postnatal) stem cells are generally identified using combinations of cell surface markers and transcription factors. However, it has been challenging to define micro-heterogeneity within “stem cell” populations, each of which stands at a different level of differentiation. As stem cells become defined at a single-cell level, their differentiation path becomes clearly defined. Here, this viewpoint discusses the potential synergy of single-cell sequencing analyses with in vivo lineage-tracing approaches, with an emphasis on practical considerations More >

TAILIN WU^1,#, XIANG ZHOU^2,#, CANHUA YE¹, WENCAN LU¹, HAITAO LIN¹, YANZHE WEI¹, ZEKAI KE¹, ZHENGJI HUANG¹, JIANZHOU LUO¹, HUIREN TAO¹, CHUNGUANG DUAN^1,*

BIOCELL, Vol.46, No.2, pp. 495-503, 2022, DOI:10.32604/biocell.2022.015214 - 20 October 2021

Abstract Differentiated macrophages have been proven to participate in the development of mesenchymal stem cells in different tissues. However, the regulatory processes remain obscure. Exosomes, which are key secretions of macrophages, have attracted increasing attention. Therefore, macrophage-derived exosomes may modulate the development of Bone marrow mesenchymal stem cells (BMMSCs). Different culture conditions were used to induce M1 polarization in THP1 cells. Subsequently, exosomes derived from unpolarized (M0) and polarized (M1) macrophages were isolated, BMMSCs were cultured with normal complete medium or inductive medium supplemented with M0 or M1 derived exosomes, and the osteogenic capacity of the… More >

XINGYU MEI, ZHOUWEI WU, CHENGZHONG ZHANG, YUE SUN, WEIMIN SHI^*

BIOCELL, Vol.45, No.6, pp. 1551-1559, 2021, DOI:10.32604/biocell.2021.015376 - 01 September 2021

Abstract Vitiligo results in an autoimmune disorder destructing skin pigment cells, melanocytes (Mcs). This study aimed to investigate whether Astragaloside IV (AIV) could efficiently induce differentiation of bone marrow mesenchymal stem cells (BMMSCs) into Mcs. BMMSCs were induced and differentiated into Mcs with 0.1, 0.2, and 0.4 mg/L AIV during 150-day. Morphologic changes of differentiated cells were observed. Levels of some melanocytic specific genes (TRP-1, TRP-2, MART-1, Mitf) were measured with quantitative polymerase chain reaction (qPCR) at 90, 120, and 150 days of induction. After 90-day induction, the differentiated cells with 0.4 mg/L AIV demonstrated the typical morphology… More >

HANXIANZHI XIAO, RONGJIA QI, ZILING WANG, MINGHE XIAO, YUE XIANG, YAPING WANG, LU WANG^*

BIOCELL, Vol.45, No.4, pp. 1045-1058, 2021, DOI:10.32604/biocell.2021.015039 - 22 April 2021

Abstract Chemotherapy may cause cellular oxidative stress to bone marrow. Oxidative damage of bone marrow hematopoietic microenvironment is closely related to chronic myelosuppression after chemotherapeutic treatment. Angelica sinensis polysaccharides (ASP) are major effective ingredients of traditional Chinese medicine Angelica with multi-target anti-oxidative stress features. In the current study, we investigated the protective roles and mechanisms of ASP on chemotherapy-induced bone marrow stromal cell (BMSC) damage. The human bone marrow stromal cell line HS-5 cells were divided into control group, 5-FU group, 5-FU + ASP group, and 5-FU + LiCl group to investigate the mechanism of ASP to… More >

Pan Zhang^1,2, Chen Zhang^1,2, Jiyang Han^1,2, Xiru Liu^1,2, Qishan Wang³, Hui Yang^1,2,4,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 132-132, 2019, DOI:10.32604/mcb.2019.06839

Abstract Research in stem cell biology relies on the knowledge of the cell microenvironment in vivo, known as “stem cell niche”, where stem cells are nurtured by the niche signals. Hematopoietic stem cells (HSCs) are capable of continuously generating and maintaining the body’s full immune and hematopoietic systems. In adult, a pool of hematopoietic cells, including HSCs, primarily reside in the bone marrow (BM) niches that plays critical roles on cell fate. Niche supporting cells, cytokines, extracellular matrix proteins and other biochemical cues associated with HSCs behaviors (quiescence, self-renewal, proliferation, differentiation, mobilization, homing, and apoptosis) has… More >

Ruikai Chen¹, Delphine Dean^1,*

Molecular & Cellular Biomechanics, Vol.14, No.3, pp. 153-169, 2017, DOI:10.3970/mcb.2017.014.153

Abstract Vascular smooth muscle cells (VSMCs) play an important role in regulating blood flow and pressure by contracting and relaxing in response to a variety of mechanical stimuli. A fully differentiated and functional VSMC should have both the ability to contract and relax in response to environmental stimuli. In addition, it should have the proper mechanical properties to sustain the mechanically active vascular environment. Stem cells can differentiate towards VSMC lineages and so could be used as a potential treatment for vascular repair. However, few studies have assessed the time it takes for stems cells to… More >

Rajasree Pia Chowdry¹, Elisa Ledet², Lahiru Ranasinghe³, Alton Oliver Sartor²

Canadian Journal of Urology, Vol.23, No.5, pp. 8483-8486, 2016

Abstract The mixed-lineage leukemia (MLL) protein acts as a histone methyltransferase regulating multiple genetic elements. Rearrangements of the MLL gene result in expression of MLL-fusion proteins that occur in some acute leukemias and are associated with poor prognosis. The MLL protein complex has been shown to interact with the androgen receptor via the MLL-menin subunit, thus promoting gene activation. The presence of MLL translocation has not been previously reported in patients with castrate resistant prostate cancer (CRPC). We describe two cases of metastatic CRPC with a translocation in the MLL gene detected by a specific fluorescent More >

Alexander C. Haab, Isabelle S. Keller, Christian Padevit, Hubert John

Canadian Journal of Urology, Vol.22, No.5, pp. 8009-8011, 2015

Abstract Ureteral stenosis due to reactivation of the BK virus (BKV) in a state of immunodeficiency is very rare. More common is the appearance of a hemorrhagic cystitis. This report not only shows bilateral ureteral stenosis after bone marrow transplantation, but also presents severe complications as chronic pelvic pain and impaired kidney function as well as irreparable damage to the whole urinary tract leading to nephroureterectomy, subtrigonal cystectomy and orthotopic ileal neobladder. Finally renal transplantation was required. To our knowledge this is the first case in the literature where such a severe course of BKV associated More >