Yinan Wang^*, Kai Xue^†, Yonghong Guan^*, Yuemei Jin^*, Shanshan Liu^*, Yichao Wang^*, Shuyan Liu^*, Ling Wang^*, Liying Han^*

Oncology Research, Vol.25, No.5, pp. 733-742, 2017, DOI:10.3727/096504016X14772362173376

Abstract Choriocarcinoma is one of the gestational trophoblastic neoplasias (GTNs) that originate in the chorionic villi and the extravillous trophoblast. Long noncoding RNAs (lncRNAs) are a type of non-protein-coding RNAs that have recently been implicated in human tumorigenesis. The present study investigated the role of the lncRNA LINC00261 in cell proliferation, metastasis, and apoptosis in choriocarcinoma cell lines. The transcription level of LINC00261 was significantly lower in choriocarcinoma tissues and in choriocarcinoma cell lines. Overexpression of LINC00261 caused a decrease in cell proliferation and arrested the cell cycle at the G₀/G₁ phase. Furthermore, overexpression of LINC00261 inhibited More >

Zhao Xiaoguang^*1,2, Liu Meirong^†1, Zhang Jingjing^‡, Zhang Ruishen^§, Zhang Qing^¶, Tan Xiaofeng^†2

Oncology Research, Vol.25, No.3, pp. 373-380, 2017, DOI:10.3727/096504016X14741486659473

Abstract The long noncoding CPS1 intronic transcript 1 (lncRNA CPS1-IT1) is a recently identified tumor suppressor in the lncRNA family of proteins. Whether this lncRNA plays any functional role in solid tumors remains largely unknown. The present study aimed to investigate the role of lncRNA CPS1-IT1 in human lung cancer. Expression of lncRNA CPS1-IT1 was initially assessed in human lung cancer and in a series of lung cancer cell lines. The effects of CPS1-IT1 overexpression on cell proliferation, migration, and invasion were examined in lung cancer cell lines A549 and 95D. It was found that lncRNA… More >

Xin Zhang^*, Shaowen Li^†, Chunli Dong^‡, Xiuying Xie^*, Yunping Zhang^*

Oncology Research, Vol.25, No.2, pp. 259-265, 2017, DOI:10.3727/096504016X14732503870766

Abstract Ovarian cancer is one of the leading causes of gynecological cancer-related deaths worldwide. We investigated the role of a newly discovered long noncoding RNA, NR_026689, in cell proliferation, metastasis, and apoptosis in ovarian cancer cells. Our results showed that NR_026689 was overexpressed in both clinical ovarian cancer patients and cultured ovarian cancer cells. Knockdown of NR_026689 in HO-8910PM cells significantly decreased the cell proliferative rate and the ability to form colonies. Transwell assays revealed that depletion of NR_026689 suppressed cell migration ability by 68% and cell invasive capacity by 71% in HO-8910PM cells. Moreover, specific More >

Xiangfei He, Fuguang Sun, Fengfu Guo, Kai Wang, Yisheng Gao, Yanfei Feng, Bin Song, Wenzhi Li, Yang Li

Oncology Research, Vol.25, No.2, pp. 157-166, 2017, DOI:10.3727/096504016X14719078133203

Abstract Renal cell carcinoma (RCC) is one of the most common kidney cancers worldwide. Although great progressions have been made in the past decades, its morbidity and lethality remain increasing. Long noncoding RNAs (lncRNAs) are demonstrated to play significant roles in the tumorigenesis. This study aimed to investigate the detailed roles of lncRNA FTX in RCC cell proliferation and metastasis. Our results showed that the transcript levels of FTX in both clinical RCC tissues and the cultured RCC cells were significantly upregulated and associated with multiple clinical parameters of RCC patients, including familial status, tumor sizes, More >

Jin G^1,2, XM Tang³, Y Niu⁴, XY Huang², T Chen², Q Huang², J Zhang², RY Zhou¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 137-142, 2017, DOI:10.32604/phyton.2017.86.137

Abstract In plants, the mitochondrial NADH dehydrogenase (complex I) is a large protein complex transferring electrons to ubiquinone. For the nine chondriogenes encoding complex I subunits (nad1, nad2, nad3, nad4, nad4L, nad5, nad6, nad7, and nad9), an efficient strategy for the cloning of complete coding sequences (CDSs) is important. Specific orthologous portions of untranslated regions (UTRs) were found based on multiple sequence alignments of chondriogene orthologues encoding complex I subunits in plant species. Based on the conservation of partial UTRs, a one-step PCR strategy was conceived for the cloning of CDSs of the nine chondriogene orthologues. Using… More >

R. Praveena¹, S. Nirmala²

CMC-Computers, Materials & Continua, Vol.53, No.1, pp. 37-47, 2017, DOI:10.3970/cmc.2017.053.039

Abstract DSP operation in a Biomedical related therapeutic hardware need to be performed with high accuracy and with high speed. Portable DSP hardware’s like pulse/heart beat detectors must perform with reduced operational power due to lack of conventional power sources. This work proposes a hybrid biomedical hardware chip in which the speed and power utilization factors are greatly improved. Multipliers are the core operational unit of any DSP SoC. This work proposes a LUT based unsigned multiplication which is proven to be efficient in terms of high operating speed. For n bit input multiplication n*n memory… More >

Xuexin Gao^*, Xuezhen Gao^†, Chao Li^*, Yukun Zhang^*, Lei Gao^‡

Oncology Research, Vol.24, No.5, pp. 337-343, 2016, DOI:10.3727/096504016X14666990347671

Abstract Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our data showed that the expression of uc.338 in lung cancer was remarkably increased in vivo and in vitro. Depletion of uc.338 with specific siRNA interference retarded the cell proliferative rate in lung cancer cell lines NCI-H929 and H1688. Furthermore, knockdown of uc.338 caused cell cycle arrest in the G₀/G₁ phase in More >

Li Wan¹, Lin Zhang¹, Kai Fan, Zai-Xing Cheng, Quan-Chao Sun, Jian-Jun Wang

Oncology Research, Vol.24, No.3, pp. 161-170, 2016, DOI:10.3727/096504016X14618564639178

Abstract As a newly identified oncogenic long noncoding RNA (lncRNA), prostate cancer-associated transcript 6 (PCAT6) promoted cellular proliferation and colony formation of prostate cancer. However, the biological function of PCAT6 in lung cancer is still largely unknown. In this study, we found that PCAT6 is significantly increased in cancer tissues compared to normal tissues and positively correlates with metastasis of lung cancer in patients. We then examined PCAT6 expression in lung cancer cell lines and identified that PCAT6 expression was significantly elevated in lung cancer cells compared to normal human bronchial epithelial (NHBE) cells, especially in… More >

Gao Liu^*, Tian Xiang^†, Quan-Feng Wu^‡, Wei-Xing Wang^*

Oncology Research, Vol.23, No.3, pp. 99-107, 2015, DOI:10.3727/096504015X14496932933575

Abstract The lncRNA H19 and its mature product miR-675 have recently been shown to be upregulated and promote the progression of gastric cancer. However, the detailed function and underlying molecular mechanism of H19/miR- 675 in the carcinogenesis of gastric cancer remains unclear. In this study, we found that H19 depended on miR- 675 to enhance the proliferation and invasion of gastric cancer AGS cells, and the expression of miR-675 was positively correlated with H19 in patients with gastric cancer. Subsequently, the tumor-suppressor runt domain transcription factor 1 (RUNX1) was confirmed to be a downstream molecule of… More >

Juan Li, Hui Meng, Yun Bai, Kai Wang

Oncology Research, Vol.23, No.5, pp. 205-217, 2015, DOI:10.3727/096504016X14549667334007

Abstract Metastasis is the primary cause of cancer-related death all over the world. Metastasis is a process by which cancer spreads from the place at which it first arose to distant locations in the body. It is well known that several steps are necessary for this process, including cancer cell epithelial–mesenchymal transition (EMT), cell migration, resistance to anoikis, and angiogenesis. Therefore, investigating the molecular mechanism of regulating cancer metastasis progress may provide helpful insights in the development of efficient diagnosis and therapeutic strategy. Recent studies have indicated that long noncoding RNAs (lncRNAs) play important roles in More >