Simiao Wang^1,#, Xue Li^1,#, Boxue Chen¹, Shitong Li¹, Jiali Wang¹, Jing Wang², Mingshuo Yang³, Xiaoyan Xu¹, Hongda Wang¹, Wenzhi Yang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 541-567, 2022, DOI:10.32604/phyton.2022.018571

Abstract The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components. The seeds of Descurainia sophia (SDS) are utilized in China as a cough and asthma relieving agent. Herein, a dimension-enhanced integral approach, by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) and intelligent peak annotation, was developed to rapidly characterize the multicomponents from SDS. Good chromatographic separation was achieved within 38 min on a UPLC CSH C18 (2.1 × 100 mm, 1.7 μm) column which was eluted by 0.1% formic acid in water (water phase) and acetonitrile (organic phase).… More >

ZHIMEI TANG, QIANG ZHANG^*

BIOCELL, Vol.46, No.2, pp. 357-366, 2022, DOI:10.32604/biocell.2022.015958

Abstract Flavonoids are a class of phytochemical molecules abundant in many plants, fruits, vegetables, and leaves. Flavonoids possess a series of significant biological activities, including anticancer, antioxidant, antiviral, and antiinflammatory properties. They become an important source of dietary supplements and natural health products. Though many studies confirmed the safety of flavonoids, the potential toxicity of flavonoids is still a remarkable field of research to be explored. The enthusiasm for flavonoids expressed by the public has sometimes overlooked their toxicity and also consumed the flavonoids exceeding the body requirements. The current review focused on the potential toxicity of flavonoids to make the… More >

JIANFEI GAO¹, KANGNING XIONG^2,*, WEIJIE LI¹, WEI ZHOU³

BIOCELL, Vol.46, No.1, pp. 285-296, 2022, DOI:10.32604/biocell.2021.016253

Abstract Kadsura coccinea (Lem.) is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds. The non-editable part of the fruit, i.e., the seed and peel, represents more than 60% of the fruit and is considered a biowaste. This significantly restricts the development of the K. coccinea fruit industry. Clarifying the metabolic components of the different fruit parts can help to improve the utilization rate and valorization of K. coccinea. Herein, we evaluated K. coccinea fruit peel, pulp, and seed using widely-targeted metabolomics and quantified a set of 736 bioactive compounds from 11 major metabolite classes. The most… More >

Olha Mykhailenko^1,*, Ivan Bezruk¹, Victoriia Volochai², Volodymyr Mishchenko³, Liudas Ivanauskas⁴, Victoriya Georgiyants¹

Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 207-221, 2022, DOI:10.32604/phyton.2022.016458

Abstract The numerous studies indicate leaves of plants are a rich source of bioactive compounds that can be a valuable source of compounds used in the pharmaceutical and cosmetic industries. Aim of this study was to investigate the chemical composition and the antioxidant property of Crocus speciosus leaves. Primary phytochemical screening of C. speciosus leaves revealed the presence of some following compound categories such as phenolic compounds, aminoacids, saponins, proteins, tannins, triterpenoids, glycosides, polysaccharides. The total flavonoids and phenolic compounds content were determined spectrophotometrically and by HPLC-DAD and HPLC-MS. Antiradical activity was determined by ABTS radical-cation scavenging method, spectrophotometrically. The total… More >

Yifu Liu^1,2, Jiazhen Wang^1,2,*, Yao Xiao¹, Xiongwen Shi¹, Yixia Zeng¹

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1549-1558, 2021, DOI:10.32604/phyton.2021.015557

Abstract The chemical compositions of tea (Camellia sinensis) are affected by numerous factors, such as cultivar, climate, leaf position, and cultivation pattern. However, under the same conditions, the chemical compositions are mainly determined by varieties. Therefore, we investigated the genetic diversity of chemical compositions of tea tree resources in China to screen excellent germplasm resources. Three chemical compositions index (including chlorophyll index, flavonoid index, and anthocyanin index) and the nitrogen balance index of tea leaves were measured in 102 tea germplasms planted in Chinese Tea Plants Improved Variety Germplasm Resources Nursery (CTPIVGRN) by Dualex on April 15 2019. Results showed that… More >

Jiao Deng^1,2,#, Fen Dong^3,#, Chaoxin Wu¹, Jiali Zhao¹, Hongyou Li¹, Juan Huang¹, Taoxiong Shi¹, Ziye Meng¹, Fang Cai¹, Qingfu Chen^1,*, Pingfang Yang^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 859-870, 2021, DOI:10.32604/phyton.2021.014625

Abstract Common buckwheat (Fagopyrum esculentum), a specialty crop in southwest China, is not only used as a supplement to primary grain crops but also to produce beverages, such as tea and wine. To fully exploit the products made from common buckwheat flower, ultra-performance liquid chromatography–electrospray ionization– tandem mass spectrometry (UPLC–ESI–MS/MS) was conducted to analyze the metabolites in red- (‘Guihong 2’) and white-flowered (‘Fengtian 1’) buckwheat cultivars. A total of 784 metabolites were identified of which flavonoids were the largest group with 191 components, followed by organic acids and derivatives (126), and amino acids and derivatives (95). Besides, dozens of phenylpropanoids, nucleotides… More >

Feng Liu^1,2,3, Zilan Ma^1,2,3, Marcos A. Caraballo-Ortiz⁴, Hui Zhang⁵, Xu Su^1,2,3, Yuping Liu^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1083-1090, 2020, DOI:10.32604/phyton.2020.011239

Abstract Pedicularis longiflora var. tubiformis (Orobanchaceae) is an abundant parasitic herb mainly found in the Xiaopohu wetland of the Qinghai Lake Basin in Northwestern China. The species has an important local medicinal value, and in this study, we evaluated the chemical profile of its stems, leaves and seeds using mass spectrometry. Dried samples of stems, leaves and seeds were grinded, weighted, and used for a series of extractions with an ultrasonic device at room temperature. The chemical profiles for each tissue were determined using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid ChromatographyMass Spectrometry (LC-MS). Twenty-seven amino acids and organic acids were identified… More >

MARÍA DE LOS A. ALVAREZ, NORA B. DEBATTISTA, NORA B. PAPPANO

BIOCELL, Vol.30, No.1, pp. 39-42, 2006, DOI:10.32604/biocell.2006.30.039

Abstract In our previous studies, bacteriostatic action of flavonoids against Staphylococcus aureus ATCC 25 923 and Escherichia coli ATCC 25 922 was demostrated. In the present work synergism of their combinations in order to improve the bacteriostatic action against the same microorganisms was determined. The experiences were made in nutritive broth, maintaining constant one drug concentration (20 µg/ml) and increasing the other one. A turbidimetric kinetic method was used and by means of a mechanism previously proposed, the minimal inhibitory concentrations (MIC´s) of each flavonoid combination were determined. The MIC´s for assayed combinations against S. aureus were: variable morin - constant… More >

QINGXIN LIU^1§ , XIANGGUI CHEN^2§, GUOLIN YANG¹ , XUEWEN MIN³ , AND MAOXIAN DENG^1,*

BIOCELL, Vol.35, No.3, pp. 71-80, 2011, DOI:10.32604/biocell.2011.35.071

Abstract Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream… More >

Cabrera M¹, M Perez², L Gallez^3,4, A Andrada³, G Balbarrey³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 124-130, 2017, DOI:10.32604/phyton.2017.86.124

Abstract Our objective was to correlate colour with total phenolic compounds, total flavonoids and antioxidant capacity of honey from the Humid Chaco region. With this purpose, 19 representative samples were selected from the 2009-2012 year period. Pollen analysis showed a predominance of native species of different landscapes such as forests and wetlands. Of the total number of samples, eleven were mixed or multifloral and eight samples were dominated by different native nectariferous woody species. The colour of honey, according to the Pfund scale, ranged from extra light amber to dark amber. Total phenolic content ranged from 40.18 to 118.82 mg GAE/100… More >