HAIFENG WANG^1,2, KAI WANG¹, JUAN GUO¹, TIEQIAO WEN²

BIOCELL, Vol.43, No.2, pp. 59-64, 2019, DOI:10.32604/biocell.2019.05731

Abstract Histone deacetylation is a key modulator involved in cell proliferation, apoptosis, and mRNA transcription. However, the effects of histone deacetylation on C17.2 neural stem cells (NSCs) remain unclear. Here, the histone deacetylase inhibitors nicotinamide and trichostatin A (TSA) were used to determine the role of histone deacetylation on gene transcription in NSCs. The results showed that the mRNA expression of p53, Sox1, Sox2, and Bax were significantly higher in E14.5 NSCs than in C17.2 NSCs. Nestin, a marker gene of neuronal differentiation, did not differ significantly between E14.5 NSCs and C17.2 NSCs. The transcription levels More >

Hala Abdulaziz M ALWARTHAN¹, Mohammad Saud AL ANAZI¹, Narasimha R. PARINE¹, Ramesa Shafi BHAT^1,*, Ghadah ALAMRO¹, Ftoon ALJARBOU¹, Sooad K AL-DAIHAN¹

BIOCELL, Vol.43, No.2, pp. 51-58, 2019, DOI:10.32604/biocell.2019.07019

Abstract Tankyrase1 plays an essential role in cancer progression by regulating telomere length. The study aimed to determine expression of TNKS1 and its regulation in colorectal cancer (CRC) in 20 samples from Saudi patients. mRNA expression of TNKS1 in CRC and paired normal tissues was measured by qRT-PCR. Epigenetic modification of TNKS1 promoter was determined by methylation-specific PCR while somatic mutation was analyzed by Sanger sequencing in exon 10 of the gene. All cancerous and normal tissues expressed TNKS1, but level of expression in CRC tissues was significantly associated with tumor stage though no other parameters; age, gender,… More >

Jie Zhong^1,#, Li Deng^2,#, Yu Jiang³, Lianhong Zou³, Huabing Yuan⁴, Shuang-xiang Tan^1,*

BIOCELL, Vol.42, No.3, pp. 99-104, 2018, DOI:10.32604/biocell.2018.04915

Abstract This study aimed to determine the effects of black tea polyphenols on gene expression in hepatocellular cancer cells. The total RNA from HepG2 hepatocellular cancer cells treated with black tea polyphenols was subjected to Human 14K cDNA microarray analysis. Real-time PCR and Western blot analysis were conducted to verify microarray data. Black tea polyphenols treatment at the dose of 20 mg/L, 40 mg/L or 80 mg/L for one to three days inhibited the growth of HepG2 cells in a dose and time dependent manner. A total of 48 genes showed more than two-fold change… More >

XiangXie^1,a, Deng Liu^1,a, Daoxi Lei¹, Yongfei Liu¹, Qi Wang¹, Zaien Wen¹, Juhui Qiu¹, Dongyu Jia^1,2, Hans Gregersen¹, Guixue Wang^1,*

Molecular & Cellular Biomechanics, Vol.14, No.3, pp. 171-186, 2017, DOI:10.3970/mcb.2017.014.171

Abstract Research in microgravity is of utmost importance for disclosing the impact of gravity on biological processes and organisms. With the development of space technology, scientists pay more attention to cardiovascular diseases associated with microgravity. However, up to date only sparse data exist on microgravity and cardiovascular development mechanisms. In this study, zebrafish was chosen as the model organism. Zebrafish embryos were exposed to microgravity using a ground-based simulation microgravity (SM) bioreactor. The effects of SM on the development of early embryonic vascular system were studied in vivo in real-time. Zebrafish embryos were selected and divided… More >

Mounir Chennaoui^1,2, Catherine Drogou^1,2, Fabien Sauvet^1,2, Danielle Gomez-Merino^1,2, Denis E. Scofield³, Bradley C. Nindl⁴

European Cytokine Network, Vol.25, No.3, pp. 52-57, 2014, DOI:10.1684/ecn.2014.0356

Abstract Acute sleep deprivation in humans has been found to increase inflammatory markers and signaling pathways in the periphery through a possible Toll-like receptor 4 (TLR-4). In addition, short duration sleep has been associated with low circulating total Insulin-like Growth Factor-I (IGF-I) concentrations. We aimed to determine whether a total sleep deprivation (TSD) protocol with recovery altered whole-blood gene expression of the proinflammatory cytokines TNF-α and IL-6, as well as TLR-4 expression, and to examine the relationship with circulating concentrations of the IGF-I system. Twelve healthy men participated in a five-day TSD (two control nights followed… More >

Hong Qian^∗, Hao Ge^†

Molecular & Cellular Biomechanics, Vol.9, No.1, pp. 1-30, 2012, DOI:10.3970/mcb.2012.009.001

Abstract Biochemical reaction systems in mesoscopic volume, under sustained environmental chemical gradient(s), can have multiple stochastic attractors. Two distinct mechanisms are known for their origins: (a) Stochastic single-molecule events, such as gene expression, with slow gene on-off dynamics; and (b) nonlinear networks with feedbacks. These two mechanisms yield different volume dependence for the sojourn time of an attractor. As in the classic Arrhenius theory for temperature dependent transition rates, a landscape perspective provides a natural framework for the system's behavior. However, due to the nonequilibrium nature of the open chemical systems, the landscape, and the attractors More >

Lukas A. Berchtold^1,2, Claus M. Larsen², Allan Vaag², Mirjam Faulenbach³, Christopher T. Workman⁴, Mogens Kruhøffer⁵, Marc Donath³, Thomas Mandrup-Poulsen^1,2,6

European Cytokine Network, Vol.20, No.2, pp. 81-87, 2009, DOI:10.1684/ecn.2009.0152

Abstract Background. We have previously reported that systemic blockade of IL-1β in patients with type 2 diabetes with anakinra (a recombinant human interleukin-1-receptor antagonist, IL-1Ra), lowered glycated hemoglobin improved beta-cell function and reduced circulating levels of IL-6 and CRP (7). To investigate the effects of IL-1Ra in insulin-sensitive tissue, gene expression levels in skeletal muscle from type 2 diabetic patients treated with IL-1Ra were analysed. Methods. Gene expression profiles in vastus lateralis muscle biop-sies from five obese patients (BMI >27) were determined before and after 13 weeks of treatment with IL-1Ra (anakinra) using Affymetrix U133Plus2.0 GeneChips.… More >

Anna M. Raskin^∗,†, Masahiko Hoshijima^†, Eric Swanson^†, Andrew D. McCulloch^*, Jeffrey H. Omens^∗,†,‡

Molecular & Cellular Biomechanics, Vol.6, No.3, pp. 145-160, 2009, DOI:10.3970/mcb.2009.006.145

Abstract Altered mechanical stress and strain in cardiac myocytes induce modifications in gene expression that affects cardiac remodeling and myocyte contractile function. To study the mechanisms of mechanotransduction in cardiomyocytes, probing alterations in mechanics and gene expression has been an effective strategy. However, previous studies are self-limited due to the general use of isolated neonatal rodent myocytes or intact animals. The main goal of this study was to develop a novel tissue culture chamber system for mouse myocardium that facilitates loading of cardiac tissue, while measuring tissue stress and deformation within a physiological environment. Intact mouse… More >

Shu Chien^*

Molecular & Cellular Biomechanics, Vol.5, No.1, pp. 1-8, 2008, DOI:10.3970/mcb.2008.005.001

Abstract Fluid shear stress due to blood flow can modulate functions of endothelial cells (ECs) in blood vessels by activating mechano-sensors, signaling pathways, and gene and protein expressions. Laminar shear stress with a definite forward direction causes transient activations of many genes that are atherogenic, followed by their down-regulation; laminar shear stress also up-regulates genes that inhibit EC growth. In contrast, disturbed flow patterns with little forward direction cause sustained activations of these atherogenic genes and enhancements of EC mitosis and apoptosis. In straight parts of the arterial tree, laminar shear stress with a definite forward More >

Donald F. Ward Jr.^*, William A. Williams^*, Nicole E. Schapiro^*, Samuel R. Christy^*, Genevieve L. Weber^*, Megan Salt, Robert F. Klees^*, Adele Boskey^†, George E. Plopper ^∗,‡

Molecular & Cellular Biomechanics, Vol.4, No.4, pp. 177-188, 2007, DOI:10.3970/mcb.2007.004.177

Abstract Focal adhesion kinase (FAK) is a key integrator of integrin-mediated signals from the extracellular matrix to the cytoskeleton and downstream signaling molecules. FAK is activated by phosphorylation at specific tyrosine residues, which then stimulate downstream signaling including the ERK1/2 pathway, leading to a variety of cellular responses. In this study, we examined the effects of FAK point mutations at tyrosine residues (Y397, Y925, Y861, and Y576/7) on osteogenic differentiation of human mesenchymal stem cells exposed to collagen I and cyclic tensile strain. Our results demonstrate that FAK signaling emanating from Y397, Y925, and to a More >