Bo Ling, Zuliang Huang, Suoyi Huang, Li Qian, Genliang Li, Qianli Tang

Oncology Research, Vol.28, No.6, pp. 561-578, 2020, DOI:10.3727/096504020X15907428281601

Abstract There is growing evidence on the clinical significance of tumor microenvironment (TME) cells in predicting prognosis and therapeutic effects. However, cell interactions in tumor microenvironments have not been thoroughly studied or systematically analyzed so far. In this study, 22 immune cell components in the lung adenocarcinoma (LUAD) TME were analyzed using gene expression profile from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). The TME-based molecular subtypes of LUAD were defined to evaluate further the relationship between molecular subtypes, prognosis, and clinical characteristics. A TME risk score model was constructed by using the More >

BICHUN ZHAO¹, XUEQING WU^2,*, YITONG YUAN¹, YUANTAO GAO¹, XIAO LI², RUOCHEN DU¹, SUMING XU², RUXIN ZHANG¹, CHUNFANG WANG^1,*

BIOCELL, Vol.44, No.4, pp. 487-499, 2020, DOI:10.32604/biocell.2020.011638 - 24 December 2020

Abstract In vitro maturation (IVM), a promising assisted reproductive technology (ART), has been evolving in clinical trials and applications. There is a huge potential demand for IVM in clinical practice because it reduces the stimulation of gonadotropins to patients and provides evidence for the safety of neonatal birth. Unfortunately, the maturation rate of oocytes in vivo is not as high as it is in vivo due to a different microenvironment. Moreover, there are still controversies in predicting the developmental capability of oocytes in IVM. The granulosa cells (GCs) and cumulus cells (CCs), closely surrounding the oocytes, play a critical… More >

REN FANG^#, WEIXIONG HUANG^#, JINYAN YAO, XING LONG, JI ZHANG, SHUANGYUN ZHOU, BIAO DENG, WENZHONG TANG, ZHENYU AN^*

BIOCELL, Vol.44, No.4, pp. 737-750, 2020, DOI:10.32604/biocell.2020.012933 - 24 December 2020

Abstract Annona squamosa is a multipurpose fruit tree employed in nutritional, medicinal, and industrial fields. Its fruit is significantly enriched in sugars, making it an excellent species to study sugar accumulation in fruit. However, the scarcity of genomic resources hinders genetic studies in this species. This study aimed at generating large-scale genomic resources in A. squamosa and deciphering the molecular basis of its high sugar content. Herein, we sequenced and characterized the full-length transcriptome of A. squamosa fruit using PacBio Iso-seq. In addition, we analyzed the changes in sugar content over five fruit growth and ripening stages, and we… More >

Swathypriyadharsini P^1,∗, K.Premalatha^2,†

Computer Systems Science and Engineering, Vol.35, No.1, pp. 39-50, 2020, DOI:10.32604/csse.2020.35.039

Abstract Microarrays have reformed biotechnological research in the past decade. Deciphering the hidden patterns in gene expression data proffers a prodigious preference to strengthen the understanding of functional genomics. The complexity of biological networks with larger volume of genes also increases the challenges of comprehending and interpretation of the resulting mass of data. Clustering addresses these challenges, which is essential in the data mining process to reveal natural structures and identify interesting patterns in the underlying data. The clustering of gene expression data has been proven to be useful in making known the natural structure inherent… More >

Cansu Altuntaş¹, Rabiye Terzi^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 487-495, 2020, DOI:10.32604/phyton.2020.08919 - 22 June 2020

Abstract The endoplasmic reticulum (ER) is an organelle in the cell where proteins are created and folded. Folding is a very elaborate process that is often interrupted by various biotic and abiotic stresses, leading to the formation of unfolded and misfolded proteins called ER stress. Dithiothreitol (DTT)-induced unfolded protein response (UPR) in endoplasmic reticulum (ER) has been recently reported in plants. Also, previous studies demonstrated that treatment with polyethylene glycol (PEG₆₀₀₀) could stimulate water deficit in crops. However, further researches should be conducted to elucidate the molecular mechanism of ER stress response and the relationship between water… More >

Yue Cai^*†‡, Chizhi Zhang^*†‡, Lei Zhan^*†, Liangbin Cheng^*†, Dingbo Lu^*†, Xiaodong Wang^*†, Hanlin Xu^§, Shuxue Wang^¶, Deng Wu^*†, Lianguo Ruan^#

Oncology Research, Vol.27, No.8, pp. 889-899, 2019, DOI:10.3727/096504018X15482423944678

Abstract The thorns of Gleditsia sinensis have been historically used in Chinese medicine and are considered one of the fundamental therapeutic herbs. Its anticancer effects are currently being explored. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and still requires the development of new drugs with higher efficiency. By using a rat HCC model implanted with cancerous Walker-256 cells, the therapeutic effects of G. sinensis extract (GSE) were assessed, as well as its regulatory effects on miRNAs. GSE significantly restored liver morphology and dramatically induced cell apoptosis in HCC rats. In addition, miR-21/181b/183 was More >

Fan Feng¹, Tingting Xia¹, Runze Zhao¹, Mengyue Wang¹, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 131-131, 2019, DOI:10.32604/mcb.2019.07211

Abstract Objective: To study the effects of different substrate stiffness on human hepatocytes using RNA sequencing (RNA-Seq) technology. The stiffness was corresponding to physiology and pathology stiffness of liver tissues.
Results: With the aid of RNA-Seq technology, our study characterizes the transcriptome of hepatocytes cultured on soft, moderate, stiff and plastic substrates. Compared to soft substrate, our RNA-Seq results revealed 1131 genes that were up-regulated and 2534 that were down-regulated on moderate substrate, 1370 genes that were up-regulated and 2677 down-regulated genes on stiff substrate. Functional enrichment analysis indicated that differentially expressed genes were associated with More >

Ning Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 21-21, 2019, DOI:10.32604/mcb.2019.07550

Abstract Decades ago YC Fung proposed that mechanical stress could have substantial impacts on remodeling and growth of living tissues. Fung also proposed the concept of residual stress in blood vessels and quantified residual stress in excised arteries [1]. However, how stress influences cell and tissue functions remains elusive. At the cellular level, we have quantified myosin II mediated pre-existing tensile stress (prestress) in living cells and demonstrated that the prestress (the endogenous cytoskeletal tension) regulates cell stiffness, gene expression, and long-distance stress propagation in the cytoplasm to activate enzymes [2]. The prestress even impacts on… More >

Xiachen Lv^1,#, Yunxia Fang^1,#, Lantian Zhang¹, Weiyi Zhang¹, Ling Xu¹, Jingjin Han¹, Bailing Jin², Xian Zhang¹, Xiaoqin Zhang^1,*, Dawei Xue^1,*

Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 91-100, 2019, DOI:10.32604/phyton.2019.06622

Abstract Melatonin (MLT) is a hormonal substance found in many organisms and can improve plant stress resistance. In this study, the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl₂ at the two-leaf stage. The growth, physiological and biochemical responses of the seedlings and the expression of cadmium (Cd)-related genes under exogenous melatonin (MLT) treatment were assessed. The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species (ROS) production and removal, resulting in ROS accumulation, membrane lipid peroxidation, and impaired growth and development. Following the… More >

Mahmoud MOUSTAFA^1,2, Saad ALAMRI¹, Hoida ZAKI², Naglaa LOUTFY², Tarek TAHA³, Ali SHATI¹, Mohamed ALKAHTANI¹, Sajda SIDDIQI¹

BIOCELL, Vol.43, No.2, pp. 81-88, 2019, DOI:10.32604/biocell.2019.06231

Abstract The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (Pisum sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases, cytoskeletal proteins and amount of amino acid in pellets of 27000 xg… More >