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  • Open Access

    ARTICLE

    Effect of Paclobutrazol Application on Plant Growth and Flower Quality in Herbaceous Peony

    Yanqing Wu1,2, Jiao Liu1, Daqiu Zhao2, Jun Tao1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 2017-2032, 2022, DOI:10.32604/phyton.2022.020643 - 13 May 2022

    Abstract Herbaceous peony (Paeonia lactiflora Pall.) is an important ornamental plant worldwide. In its natural state, P. lactiflora often manifests traits like rapidly elongating internodal growth, loose plant types, and soft inflorescence stems. However, very little has been known about the measures for controlling these traits. This study investigated the effect of applying paclobutrazol (PBZ) on the plant growth and flower quality in P. lactiflora. The results indicated that PBZ application reduced the plant height (8.05%), plant crown width (14.72%), and leaf area (10.90%), but increased the leaf thickness (18.18%) and stem diameter (over 11%) in P. lactiflora. Meanwhile, PBZ… More >

  • Open Access

    ARTICLE

    Headspace Solid-Phase Micro-Extraction Gas Chromatography/Mass Spectrometry (HS-SPME-GC/MS)-Based Untargeted Metabolomics Analysis for Comparing the Volatile Components from 12 Panax Herbal Medicines

    Simiao Wang1,#, Xiaohang Li1,#, Meiting Jiang1, Xinlong Wu2, Yuying Zhao1, Meiyu Liu1, Xiaoyan Xu1, Huimin Wang1, Hongda Wang1, Heshui Yu2, Wenzhi Yang1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1353-1364, 2022, DOI:10.32604/phyton.2022.021093 - 14 March 2022

    Abstract Quality control of ginseng currently is mainly based on ginsenoside analysis, but rarely focuses on the volatile organic components. In the current work, an untargeted metabolomics approach, by headspace solid-phase micro-extraction gas chromatography/mass spectrometry (HS-SPME-GC/MS), was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines, which included P. ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), red ginseng (PGR), P. ginseng leaf (PGL), P. quinquefolius leaf (PQL), P. notoginseng leaf (PNL), P. ginseng flower (PGF), P. quinquefolius flower (PQF), P. notoginseng flower (PNF), P. japonicus (PJ), and P. japonicus var. major (PJvm). Chromatographic separation was performed… More >

  • Open Access

    ARTICLE

    Integrated Analysis of Metabolome and Transcriptome of Bambusa emeiensis Shoots in Response to Bamboo Snout Beetle Cyrtotrachelus buqueti (Coleoptera: Curculionidae)

    Hao Tang#, Yuanqiu Li#, Chaobing Luo*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1227-1244, 2022, DOI:10.32604/phyton.2022.019278 - 14 February 2022

    Abstract Plants have evolved constitutive and inductive defense mechanisms to protect themselves from herbivorous insects. Metabolites in plants are thought to be involved in response to herbivores. Bambusa emeiensis is an important bamboo species widely distributed in Southwest China. It is easy to attract herbivores such as Cyrtotrachelus buqueti. Through the detection and analysis of metabolites in B. emeiensis metabolomic data, 35 differential metabolites (biomarkers) were finally identified from 206 detection peaks, mainly primary metabolites. Subsequently, we conducted an association analysis between 35 biomarkers that annotated to be involved in 71 metabolic pathways in the metabolome and 324 differentially… More >

  • Open Access

    ARTICLE

    Overexpression of a Glycosyltransferase Gene from a Metabolically Poly-Resistant Beckmannia syzigachne Population Alters Growth and Confers Herbicide Resistance to Brachypodium distachyon

    Yizhao Huang1, Nannan Li2, Deya Wang4, Jian Du1, Weitang Liu3, Jinxin Wang3,*, Wei Li1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 761-772, 2022, DOI:10.32604/phyton.2022.018462 - 09 December 2021

    Abstract Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China. The B. syzigachne (AH-02) population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl. To investigate the function of GT73C1 in this population, the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction, and the sequence was 100% consistent with the transcriptome data. Its phylogenetic tree was displayed and annotated using FigTree v1.4.4. The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants. Furthermore, the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl, which was consistent with the findings from the whole plant More >

  • Open Access

    ARTICLE

    Allelopathic Potential and Mechanism of Rosebay Willowherb [Chamaenerion angustifolium (L.) Scop.] Demonstrated on Model Plant Lettuce

    Hailin Shi#, Shiwei Sun#, Xiaohong Liu, Jiahe Fan, Jin Wang, Ke Zhao, Wei Wang*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 159-170, 2021, DOI:10.32604/phyton.2020.011620 - 20 November 2020

    Abstract Allelopathic plants are important resources for the discovery of bioherbicides. Rosebay willowherb [Chamaenerion angustifolium (L.) Scop. syn. Epilobium angustifolium L.] widely distributes in Western Asia, Europe, and North America, and behaves as a dominant species within the community due to the production of substances that restrict growth of other plants. This study aims at investigating the allelopathic potential of rosebay willowherb by evaluation of the effects of aqueous extracts from different parts on seed germination and seedling growth in lettuce (Lactuca sativa L.), as well as measuring the accumulation of reactive oxygen species and structural analysis of root… More >

  • Open Access

    ARTICLE

    Phyllanthus niruri (stone breaker) herbal therapy for kidney stones; a systematic review and meta-analysis of clinical effcacy, and Google Trends analysis of public interest

    Survandita Dhawan1, Ephrem O. Olweny2

    Canadian Journal of Urology, Vol.27, No.2, pp. 10162-10166, 2020

    Abstract Introduction: Phyllanthus niruri (P. niruri) is the most commonly listed active ingredient in commercially available herbal therapies for kidney stones, despite limited supporting clinical evidence. We performed a meta-analysis to evaluate its efficacy in reducing stone burden. We used Google Trends to analyze its relative popularity in internet searches relative to conventional stone therapies.
    Materials and methods: A comprehensive literature search for controlled human studies containing data on the effect of P. niruri treatment on stone size and number was performed. Pooled analysis of change in mean stone size and number with P. niruri was performed using… More >

  • Open Access

    ARTICLE

    2,4-D Hyper Accumulation Induced Cellular Responses of Azolla pinnata R. Br. to Sustain Herbicidal Stress

    Arnab Kumar De, Arijit Ghosh, Debabrata Dolui, Indraneel Saha, Malay Kumar Adak*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 999-1017, 2020, DOI:10.32604/phyton.2020.010828 - 09 November 2020

    Abstract In the present experiment with ongoing concentration (0 µM, 100 µM, 250 µM, 500 µM and 1000 µM) of 2,4-D, the responses of Azolla pinnata R.Br. was evaluated based on cellular functions. Initially, plants were significantly tolerated up to 1000 µM of 2,4-D with its survival. This was accompanied by a steady decline of indole acetic acid (IAA) concentration in tissues with 78.8% over the control. Membrane bound H+ -ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator (KCl) and decreased within a range of 57.3 to 74.6% in response to… More >

  • Open Access

    ARTICLE

    Chemical Constituents of Pedicularis longiflora var. tubiformis (Orobanchaceae), a Common Hemiparasitic Medicinal Herb from the Qinghai Lake Basin, China

    Feng Liu1,2,3, Zilan Ma1,2,3, Marcos A. Caraballo-Ortiz4, Hui Zhang5, Xu Su1,2,3, Yuping Liu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 1083-1090, 2020, DOI:10.32604/phyton.2020.011239 - 09 November 2020

    Abstract Pedicularis longiflora var. tubiformis (Orobanchaceae) is an abundant parasitic herb mainly found in the Xiaopohu wetland of the Qinghai Lake Basin in Northwestern China. The species has an important local medicinal value, and in this study, we evaluated the chemical profile of its stems, leaves and seeds using mass spectrometry. Dried samples of stems, leaves and seeds were grinded, weighted, and used for a series of extractions with an ultrasonic device at room temperature. The chemical profiles for each tissue were determined using Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid ChromatographyMass Spectrometry (LC-MS). Twenty-seven amino acids and organic… More >

  • Open Access

    ARTICLE

    Experimental and In Silico Analysis of Cordycepin and its Derivatives as Endometrial Cancer Treatment

    Pedro Fong*1, Cheng N. Ao*†1, Kai I. Tou*, Ka M. Huang*, Chi C. Cheong*, Li R. Meng*

    Oncology Research, Vol.27, No.2, pp. 237-251, 2019, DOI:10.3727/096504018X15235274183790

    Abstract The aim of this study was to investigate the inhibition effects of cordycepin and its derivatives on endometrial cancer cell growth. Cytotoxicity MTT assays, clonogenic assays, and flow cytometry were used to observe the effects on apoptosis and regulation of the cell cycle of Ishikawa cells under various concentrations of cordycepin, cisplatin, and combinations of the two. Validated in silico docking simulations were performed on 31 cordycepin derivatives against adenosine deaminase (ADA) to predict their binding affinities and hence their potential tendency to be metabolized by ADA. Cordycepin has a significant dose-dependent inhibitory effect on More >

  • Open Access

    ARTICLE

    The Allelopathic Effects of Sunflower and Wheat Root Exudates on Sinapis arvensis and Sinapis alba

    Bengu Turkyilmaz Unal1,*, Mustafa Bayram2

    Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 413-423, 2019, DOI:10.32604/phyton.2019.08244

    Abstract In this study, we aimed to investigate the allelopathic effects of sunflower and wheat root exudates on the common weeds such as wild mustard and white mustard in our region. The root exudates which were obtained by soaking 8 weeks old sunflower and wheat seedlings (20 or 40 seedlings) in 100 mL of distilled water for 3 days were applied to the leaves of wild mustard and white mustard. In order to compare the allelopathic effect, the recommended dose (1 g.da-1 ) and twice the recommended dose (2 g.da-1 ) of Gromstor (Tribenuron-methyl), a herbicide preferred… More >

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