Nada M. Mostafa^1,5,#, Muhammad I. Ismail^2,#, Amr M. El-Araby³, Dina M. Bahgat¹, Ahmed M. Elissawy^1,5, Ahmed M. Mostafa⁴, Omayma A. Eldahshan^1,5,*, Abdel Nasser B. Singab^1,5,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1089-1104, 2022, DOI:10.32604/phyton.2022.018239 - 24 January 2022

Abstract Coronaviruses caused an outbreak pandemic disease characterized by a severe acute respiratory distress syndrome leading to the infection of more than 200 million patients and the death of more than 4 million individuals. The primary treatment is either supportive or symptomatic. Natural products have an important role in the development of various drugs. Thus, screening of natural compounds with reported antiviral activities can lead to the discovery of potential inhibitory entities against coronaviruses. In the current study, an in-silico molecular docking experiment was conducted on the effects of some of these natural antiviral phytoconstituents, (e.g.,… More >

TIANTIAN XU^1,#, RUILIN ZHENG^1,#, DANYANG CHEN^1,#, HAIYANG CHEN², MINGQI ZHAO¹, MIN GUO¹, YI CHEN¹, CHANGBING WANG¹, LU KUANG¹, YINGHUA LI^1,*, BING ZHU^1,*

BIOCELL, Vol.46, No.4, pp. 1033-1039, 2022, DOI:10.32604/biocell.2022.017757 - 15 December 2021

Abstract Emerging evidence that Enterovirus A 71 (EV-A71) infection closely related to apoptosis. The ZVAD is a caspase inhibitor that can prevent apoptosis. The aims of this project were to evaluate the mechanism of the ZVAD inhibited EV-A71 virus and to provide experiment basis for finding new antiviral drugs. In this study, after treated with ZVAD in EV-A71 infected Vero cells, the viral replication was reduced, and the cell viability was higher than EV-A71 group. Additionally, ZVAD decreased the cell apoptosis and the level of inflammatory cytokines induced by EV-A71 in the infected Vero cells. ZVAD More >

HONGXIA HU^*, PING GUO, QIAN ZHAO, HAORAN LI, HUALEI LIU, CAIHONG MA

BIOCELL, Vol.45, No.4, pp. 995-1004, 2021, DOI:10.32604/biocell.2021.012851 - 22 April 2021

Abstract This study aims to detect the expression of selected circulating microRNAs (miRNA), including miRNA-451a, miRNA-486-5p and miR-10b-5p, and their prospective roles as biomarkers in patients with atherosclerosis. For this purpose, levels of miRNAs were detected by real-time quantitative polymerase chain reaction (RT-qPCR) in case (N = 30) and healthy control (N = 30) groups. Receiver operating characteristic (ROC) curve analysis was carried out to evaluate the diagnostic ability of miRNAs. The correlations of miR-451a with lipid parameters were evaluated using Pearson’s correlation coefficients. HUVEC tested by ox-LDL was used as a cellular model of atherosclerosis.… More >

Roberto Arredondo-Valdés¹, Francisco D. Hernández-Castillo², Mario Rocandio-Rodríguez¹, Julia C. Anguiano-Cabello³, Madai Rosas-Mejía¹, Venancio Vanoye-Eligio¹, Salvador Ordaz-Silva⁴, Imelda V. López-Sánchez⁴, Laura D. Carrazco-Peña⁴, Julio C. Chacón-Hernández^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 895-906, 2021, DOI:10.32604/phyton.2021.014301 - 30 March 2021

Abstract The tomato (Solanum lycopersicum L.) is one of the world’s most important vegetable crops. Still, phytopathogenic bacteria affect the yield and quality of tomato cultivation, like Agrobacterium tumefeciens (At), Clavibacter michiganensis subsp. michiganensis (Cmm), Pseudomonas syringae pv. tomato (Pst), Ralstonia solanacearum (Rs), and Xanthomonas axonopodis (Xa). Synthetic chemical products are used mostly on disease plant control, but overuse generates resistance to bacterial control. This study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera Lam. leaves against At, Cmm, Pst, Rs, and Xa, as well as information about this plant species’ chemical composition. Antibacterial activity against pathogens observed by microplate technique, phytochemical screening, More >

JUNQI GUO^1,2,#, YUN YANG^1,2,#, WEI ZHAO^1,2, ZHONGHAI YAN³, XIA YANG⁴, YUNFEI YAN^1,2, RUIMIN HAO^1,2, JINXIA HU^1,2,*, FEI JIAO^1,2,*

BIOCELL, Vol.45, No.3, pp. 627-638, 2021, DOI:10.32604/biocell.2021.013496 - 03 March 2021

Abstract Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression. However, the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer (NSCLC) are not to be well studied. Here, we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines. The correlation between the clinicopathological features of NSCLC and the miR-16- 5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage, positive lymph node metastasis, with short overall survival (OS). Also, a negative More >

Wilber Montejo-Mayo¹, Eduardo Días-Rivera¹, Mauro Martínez-Pacheco², Abril Munro-Rojas¹, Enrique Ambriz-Parra¹, Crisanto Velázquez-Becerra^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 193-206, 2021, DOI:10.32604/phyton.2020.011402 - 20 November 2020

Abstract Wood is an organic material that is a source of carbon of organisms called Wood-decay fungi, and to preserve the wood, various toxic compounds to man and the environment have been used. To analyze the effect of N,N-Dimethylhexadecylamine (DMHDA) on wood attacked by the rotting fungus Trametes versicolor L. We used an in vitro system to expose the fungus T. versicolor to different concentrations of the DMHDA (50, 150 and 450 μM). We quantified the diameter of mycelial growth and laccase activity, also, under these experimental conditions we studied morphological details of the organisms using different scanning equipment including scanning… More >

Kashaf Junaid^1,*, Sumera Qasim², Humaira Yasmeen³, Hasan Ejaz¹, Abdullah Alsrhani¹, Muhammad Ikram Ullah¹, Fahad Ahmad⁴, Abdul Rehman⁵

CMC-Computers, Materials & Continua, Vol.66, No.1, pp. 707-714, 2021, DOI:10.32604/cmc.2020.012976 - 30 October 2020

Abstract Coronavirus disease 2019 (COVID-19) is a current pandemic that has affected more than 195 countries worldwide. In this severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, when treatment strategies are not yet clear and vaccines are not available, vitamins are an excellent choice to protect against this viral infection. The rationale behind this study was to examine the inhibitory effect of vitamins B, C, and D against the main protease of SARSCoV-2 and angiotensin-converting enzyme 2 (ACE2), which have critical rolesin the immune system. Molecular docking, performed by using MOE-Dock of the Chemical Computing Group,… More >

SAIDA MELIANI¹, SOUMAYA TOUMI¹, HEYTHEM DJAHOUDI², KHALED DEGHDEGH³, KAMEL AMOURA⁴, ABDELGHANI DJAHOUDI^5,*

BIOCELL, Vol.44, No.2, pp. 175-182, 2020, DOI:10.32604/biocell.2020.09097 - 27 May 2020

Abstract Objective: The aim of this study is to detect in vitro the synergetic activity of colistin in combination with imipenem, amikacin or ciprofloxacin, at sub-inhibitory concentrations, against carbapenems-resistant (CR) Acinetobacter baumannii and Pseudomonas aeruginosa strains isolated from various wards in Annaba teaching hospital in eastern Algeria.
Materials and Methods: The minimal inhibitory concentrations (MIC) were determined by broth macrodilution (BMD). Carbapenemase encoding genes were screened using polymerase chain reaction (PCR). The activity of colistin in combination with second antibiotic was evaluated by the Checkerboard Technique.
Results: 39 CR P. aeruginosa and 21 CR A. baumanni strains where collected. The MIC values ranging… More >

Enrico Mini^*, Ida Landini^*, Laura Lucarini^†, Andrea Lapucci^*, Cristina Napoli^‡, Gabriele Perrone^*, Renato Tassi^*, Emanuela Masini^†, Flavio Moroni^†, Stefania Nobili^‡

Oncology Research, Vol.26, No.2, pp. 333-334, 2018, DOI:10.3727/096504018X15187172557369

Abstract The poly(ADP-ribose) polymerase (PARP) enzymes play a key role in the regulation of cellular processes (e.g., DNA damage repair, genomic stability). It has been shown that PARP inhibitors (PARPIs) are selectively cytotoxic against cells having dysfunctions in genes involved in DNA repair mechanisms (synthetic lethality). Drug-induced PARP inhibition potentiates the activity of anticancer drugs such as 5-fluorouracil in enhancing DNA damage, whose repair involves PARP-1 activity. The aim of this study was to evaluate the inhibitory effects of a novel PARPI, HYDAMTIQ, on growth in human tumor cell lines characterized by different features with regard… More >

Christian Paech, Franziska Wagner, Sebastian Mensch, Roman Antonin Gebauer

Congenital Heart Disease, Vol.13, No.6, pp. 1064-1068, 2018, DOI:10.1111/chd.12682

Abstract Introduction: Reflex vasovagal—or cardioinhibitory syncope is known to be a major cause of recurrent syncope in children. The mechanism of vasovagal syncope (VVS) is an interaction between a vagally mediated bradycardia or asystole and a more or less manifest vasodilatory component. Although pacing is not advisable as a standard approach in patients with VVS, it remains a treatment option of last resort in excep‐ tionally severe cases, or patients with contraindication or refractoriness to drug ther‐ apy and life style changes. To effectively avoid VVS in these patients, the pacemaker has to both prevent bradycardia and… More >