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  • Open Access

    ARTICLE

    Relationship between leaf greenness index and leaf water status in wheat under different tillage systems

    Mirassón1 HR, ML Faraldo1, MN Fioretti2, M Miravalles2, RE Brevedan2

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 183-187, 2010, DOI:10.32604/phyton.2010.79.183

    Abstract Tillage system is an important management decision that has to be taken in the central, semiarid, temperate region of Argentina for dryland wheat production. A 2-year study was conducted on an Entic Haplustoll soil with a sandy loam texture to determine the effects of three tillage systems (conventional, vertical and notillage) on leaf water status and chlorophyll content. Measurements were made at the Z65 growth stage with a SPAD-502; leaf water potential measurements were simultaneously taken. There was a positive relationship between leaf water potential and leaf greenness in both years. Tillage systems were significantly More >

  • Open Access

    ARTICLE

    Leaf angle and light interception in sunflower (Helianthus annuus L.). Role of the petiole’s mechanical and anatomical properties

    Hernández LF

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 109-115, 2010, DOI:10.32604/phyton.2010.79.109

    Abstract The relationships between (1) leaf biomass and morphology (lamina area and petiole and lamina inclination), (2) petiole’s mechanical and structural properties, and (3) the vertical light gradient inside the crop’s canopy were studied in field grown sunflower (Helianthus annuus L.) plants, maintained at optimum soil water and mineral levels. At flowering, incident photosynthetic active radiation (PAR) was measured at the top of the canopy and on individual leaves using a quantum sensor. The fraction of direct incident radiation which passed through the canopy reaching each individual leaf was then calculated. Individual petiole and lamina inclination angles (iaPetioleMore >

  • Open Access

    ARTICLE

    Induced responses in the subtropical evergreen, broad-leaf tree Schima superba: Effects of simulated herbivory on leaf quality and subsequent insect attack during leaf expansion

    Liu ZG1,2, YL Cai1,2, K Li2,3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 81-86, 2010, DOI:10.32604/phyton.2010.79.081

    Abstract Induced responses to herbivory are physical, nutritional, and allelochemical traits that change in plants following disturbances, and reduce the performance and/or preference of leaf tissues on herbivores. This study gave evidence to the induced defense theory through the simulated herbivory in Schima superba, one of common dominant trees in subtropical evergreen, broadleaf forests in southern China. Results showed that leaves damaged at the beginning of leaf expansion would develop into having a larger area, higher toughness and higher tannin concentrations, but a lower water content compared with control leaves. As a result, they experienced lower herbivory More >

  • Open Access

    ARTICLE

    Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture

    MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

    BIOCELL, Vol.33, No.3, pp. 179-186, 2009, DOI:10.32604/biocell.2009.33.179

    Abstract With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of More >

  • Open Access

    ARTICLE

    Leaf blade anatomy and ultrastructure of six Simira species (Rubiaceae) from the Atlantic Rain Forest, Brazil

    TARSILA MARIA DA SILVA MORAES1, CLAUDIA FRANCA BARROS2, SEBASTIÃO JOSÉ DA SILVA NETO3, VALDIRENE MOREIRA GOMES4, MAURA DA CUNHA1*

    BIOCELL, Vol.33, No.3, pp. 155-165, 2009, DOI:10.32604/biocell.2009.33.155

    Abstract Simira is a predominantly woody Neotropical genus comprising 41 taxa, 16 of which occur in Brazil and eight of them in the southeastern region of Brazil. Leaf blades of Simira eliezeriana Peixoto, S. glaziovii (K. Schum.) Steyerm., S. grazielae Peixoto, S. pikia (K. Schum.) Steyerm., S. rubra (Mart.) Steyerm., S. sampaioana (Standl.) Steyerm. were collected in the southeastern region of Brazil and fixed according to usual methods for light and electron microscopy. The leaf blades show typical characteristics of the Rubiaceae family as dorsiventral mesophyll and paracytic stomata. The presence of two bundle sheaths that extend to the upper epidermal layer, More >

  • Open Access

    ARTICLE

    Quantitative measures of leaf epidermal cells as a taxonomic and phylogenetic tool for the identification of Stanhopea species (Orchidaceae)

    Foroughbakhch R, RJ Ferry Sr, JL Hernández-Piñero, MA Alvarado-Vázquez, A Rocha-Estrada

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 113-127, 2008, DOI:10.32604/phyton.2008.77.113

    Abstract Orchids of the genus Stanhopea are currently identified only by their floral structure characteristics. A statistical analysis of a significant number of species of this genus disclosed that measurements of adaxial and abaxial epidermal cell surface areas can be correlated with specific recognized species by a leaf printing method. This allows an objectively either positive or nearly positive confirmation of the identity of a species in the absence of flowers and without damage to plants. When ordering the mean values obtained for these surface areas in each species in a decreasing order, a correlation was observed More >

  • Open Access

    ARTICLE

    Genetic and resistance stability to Black Sigatoka disease during micropopagation of Musa CIEN BTA-03 somaclonal variant

    Giménez1 C, E de García2, O Haddad3

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

    Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from More >

  • Open Access

    ARTICLE

    Variability in leaf morphology and anotomy in Lippia turbinata (Verbenaceae) in the Province of San Luis (Argentina)

    Andersen A, Lucchini F, J Moriconi, EA Fernández

    Phyton-International Journal of Experimental Botany, Vol.75, pp. 137-143, 2006, DOI:10.32604/phyton.2006.75.137

    Abstract Medicinal plants with mesomorphic and xeromorphic characteristics are a significant component of the indigenous flora of San Luis province. In popular medicine, Lippia turbinata “poleo” has emmenagogic, diuretic and stomach properties.
    The morphological and anatomical aspects of foliar leaves were studied according to their environmental conditions, in order to contribute to the identification of samples of commercial drugs. From the studied sections, differences in the following aspects were detected, namely the form and type of epidermic cells, the presence of idioblasts in adaxial epidermic position, thickness of cuticle, density of nonglandular trichomes, the structure of the More >

  • Open Access

    ARTICLE

    Influence of genotype and explant source on indirect organogenesis by in vitro culture of leaves of Melia azedarach L.

    S.K. VILA*, H.Y. REY, L.A. MROGINSKI

    BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

    Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l-1 benzylaminopurine (BAP) + 0.1mg.l-1 kinetin (KIN) + 3 mg.l-1 adenine sulphate (ADS).
    The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the More >

  • Open Access

    ARTICLE

    Water relations and leaf growth rate of three Agropyron genotypes under water stress

    MARÍA G. GARCÍA, CARLOS A. BUSSO, PABLO POLCI, NORBERTO L. GARCÍA GIROU, VIVIANA ECHENIQUE

    BIOCELL, Vol.26, No.3, pp. 309-317, 2002, DOI:10.32604/biocell.2002.26.309

    Abstract The effects of water stress on leaf water relations and growth are reported for three perennial tussock grass genotypes under glasshouse conditions. Studies were performed in genotypes El Palmar INTA and Selección Anguil of Agropyron scabrifolium (Döell) Parodi, and El Vizcachero of A. elongatum (Host) Beauv. Agropyron scabrifolium El Palmar INTA is native to a region with warm-temperate and humid climate without a dry season, and an average annual precipitation of 900 mm. Agropyron scabrifolium Selección Anguil comes from a region with a sub-humid, dry to semiarid climate and a mean annual precipitation of 600 mm. Agropyron elongatum is a… More >

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