Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (128)
  • Open Access

    ABSTRACT

    Ultra-stable Biomembrane Force Probe to Characterize Strong Protein-Protein Interactions on a Living Cell

    Chenyi An1, Wei Chen2,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 109-110, 2019, DOI:10.32604/mcb.2019.07634

    Abstract Biomembrane force probe (BFP) is a single-molecule biomechanical technique that has been widely used to characterize protein dynamics (e.g., protein-protein interactions and protein conformational changes), especially suitable for measuring force-regulated receptor-ligand binding kinetics in situ[1-4]. Integrated with various force spectroscopies, such as lifetime assay, it has become a powerful platform to systematically characterize many force-regulated receptor-ligand dissociation of great biological significance, which cannot be done with traditional solution based assays (e.g., surface plasma resonance) [5].
    Even though the BFP has been quite successful in characterizing binding kinetics of weak and transient molecular interactions, it is… More >

  • Open Access

    ABSTRACT

    Effect of Protein-Induced Membrane Curvature on the Receptor-Ligand Binding Constant

    Long Li1, Jinglei Hu2, Fan Song1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 54-55, 2019, DOI:10.32604/mcb.2019.07718

    Abstract Cell adhesion is a fundamental biological process involved in many crucial cellular activities such as tissue formation, immune response, and cell locomotion [1, 2]. The adhesion process is mediated by the specific binding of membrane-anchored receptor and ligand proteins, which is quantified by the two-dimensional binding equilibrium constant [3-5]. These adhesion proteins are associated with cell membranes either via transmembrane domains or via GPI anchors, and may very likely generate membrane curvature, which has been shown for a number of membrane proteins to play an important role in organelle shaping, vesicle trafficking, cell fusion and… More >

  • Open Access

    ABSTRACT

    Numerical Analysis of Motion and Stress Distribution of Circulating Tumor Cells in Micro Vessels

    Peng Jing1, Xiaolong Wang1, Shigeho Noda2, Xiaobo Gong1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 36-37, 2019, DOI:10.32604/mcb.2019.07111

    Abstract The motion of circulating tumor cells (CTCs) in microcirculatory system is one of the critical steps during cancer metastasis. The moving behavior and stress distribution of circulating tumor cells under different geometry and flow conditions are important basis for studying the adhesion between circulating tumor cells and vessel walls. In the present work, the motion and deformation of circulating tumor cells in capillary tubes are numerically studied using the immersed boundary method (IBM). The membrane stress distribution of CTCs in confined tubes are investigated with under vessel diameters, hematocrit (Ht) values and capillary numbers (Ca). More >

  • Open Access

    ABSTRACT

    Multiscale Modeling of Clathrin-Mediated Endocytosis

    Padmini Rangamani1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 22-22, 2019, DOI:10.32604/mcb.2019.08513

    Abstract Endocytosis is the process of uptake of cargo and fluid from the extracellular space to inside the cell; defects in endo- cytosis contribute to a wide spectrum of diseases including cancer, neurodegeneration, and heart disease. Clathrin- mediated endocytosis (CME) is an archetypal example of a membrane deformation process where multiple variables such as pre-existing membrane curvature, membrane bending due to the protein machinery, membrane tension regulation, and actin-mediated forces govern the progression of vesiculation. My group has been working for the past few years on deciphering the biophysical determinants of CME using multiscale modeling. We… More >

  • Open Access

    ABSTRACT

    Autodigestion in Physiological Shock, Organ Dysfunction and Death

    Erik B. Kistler1, Geert W. Schmid-Schönbein2,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 8-8, 2019, DOI:10.32604/mcb.2019.07256

    Abstract A longstanding question in research on organ failure after physiological shock (such as trauma, burns, sepsis, surgery and medical emergencies) is the underlying mechanism for a progressive loss of cell and tissue functions. Our systematic analysis of this problem has served to identify digestive enzymes as key players [1, 2]. After synthesis and discharge from the pancreas, the digestive enzymes are usually contained inside the lumen of the small intestine where they break down food every day. Escape of the digestive enzymes out of the lumen of the intestine is kept to a minimum by… More >

  • Open Access

    ABSTRACT

    A Multicomponent Transport Model for Proton Exchange Membrane Fuel Cells Using Ordered Membrane Electrode Assembly

    Jiarong Liang1, Yinshi Li1,2,*, Jinghui Jiang1

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.22, No.3, pp. 168-168, 2019, DOI:10.32604/icces.2019.05679

    Abstract Designing cost-effective electrodes is essential to the development of proton exchange membrane fuel cells (PEMFCs). Therein, the highly ordered electrode has been proved to be effective by reducing Pt loading. To insight into the enhancement mechanism of ordered membrane electrode assembly (OMEA), in this work, a two-dimensional steady-state multicomponent mass-transport model is developed, in which a cylindrical structure model is adapted to account for the diffusion characteristics of reactants in ordered catalyst layer. Moreover, the overpotential corresponding to mass and charge transport limitations are calculated in this model based on concentration distribution and Ohm’s law,… More >

  • Open Access

    ARTICLE

    Functionalised Poly(Vinyl Alcohol)/Graphene Oxide as Polymer Composite Electrolyte Membranes

    O. Gil-Castell1,2, R. Cerveró1, R. Teruel-Juanes1, J. D. Badia1,2, A. Ribes-Greus1,*

    Journal of Renewable Materials, Vol.7, No.7, pp. 655-665, 2019, DOI:10.32604/jrm.2019.04401

    Abstract Crosslinked poly(vinyl alcohol) (PVA) based composite films were prepared as polyelectrolyte membranes for low temperature direct ethanol fuel cells (DEFC). The membranes were functionalised by means of the addition of graphene oxide (GO) and sulfonated graphene oxide (SGO) and crosslinked with sulfosuccinic acid (SSA). The chemical structure was corroborated and suitable thermal properties were found. Although the addition of GO and SGO slightly decreased the proton conductivity of the membranes, a significant reduction of the ethanol solution swelling and crossover was encountered, more relevant for those functionalised with SGO. In general, the composite membranes were More >

  • Open Access

    ARTICLE

    Novel Membranes Regenerated from Blends of Cellulose/Gluten Using Ethylenediamine/Potassium Thiocyanate Solvent System

    Yang Yu1, Ramiz Boy1,2,*, Richard Kotek1

    Journal of Renewable Materials, Vol.7, No.1, pp. 41-55, 2019, DOI:10.32604/jrm.2019.00105

    Abstract Current industrial methods for dissolution of cellulose in making regenerated cellulose products are relatively expensive, toxic and dangerous and have environmental problems coming with the hazard chemical wastes. To solve these problems, a novel ethylenediamine and potassium thiocyanate (ED/KSCN) solvent system was developed, that is economical, ecofriendly, and highly efficient. The ED/KSCN solvent system was proven to be a suitable solvent for fabricating cellulose (blended with other polymers) membranes. In this study, gluten was used to develop nonporous membranes with cellulose. The method of casting these membranes provided better ones than the former researchers’ techniques. More >

  • Open Access

    ARTICLE

    Oncogenic Role of MicroRNA-30b-5p in Glioblastoma Through Targeting Proline-Rich Transmembrane Protein 2

    Zhongjun Li*, Junxiu Guo*, Yujie Ma, Longbo Zhang, Zhixiong Lin*

    Oncology Research, Vol.26, No.2, pp. 219-230, 2018, DOI:10.3727/096504017X14944585873659

    Abstract MicroRNAs (miRs) have been found to play promoting or suppressive roles in different human cancers. However, the exact regulatory mechanism of miR-30b in glioblastoma remains unknown. Here we have shown that the expression of miR-30b is significantly increased in glioblastoma tissues and cell lines. Moreover, a high expression of miR-30b is significantly associated with a shorter survival time for glioblastoma patients. Knockdown of miR-30b caused a significant reduction in the proliferation, migration, and invasion of U87 and A172 cells. Proline-rich transmembrane protein 2 (PRRT2) was further identified as a novel target gene of miR-30b, and More >

  • Open Access

    ARTICLE

    Knockdown of TMPRSS3, a Transmembrane Serine Protease, Inhibits Proliferation, Migration, and Invasion in Human Nasopharyngeal Carcinoma Cells

    Jun-Ying Wang*, Xin Jin*, Xiao-Feng Li

    Oncology Research, Vol.26, No.1, pp. 95-101, 2018, DOI:10.3727/096504017X14920318811695

    Abstract TMPRSS3 belongs to the large type II transmembrane serine protease (TTSP) family, which plays an important role in the development and progression of tumors. However, the function of TMPRSS3 in nasopharyngeal carcinoma (NPC) remains unclear. The present study aimed to examine the impact of TMPRSS3 on the proliferation, migration, and invasion of NPC cells and their potential mechanisms. Our results demonstrated that the expression of TMPRSS3 was obviously upregulated in human NPC tissues and cell lines. Knockdown of TMPRSS3 expression significantly suppressed the proliferation and tumorigenicity of NPC cells in vitro and in vivo. Furthermore, More >

Displaying 61-70 on page 7 of 128. Per Page