YUE GUAN^1,#, TINGTING ZHANG^2,#, JIANAN YU³, JIAWEI LIU⁴, WENYUAN LI⁵, YUJIA ZHENG⁶, JIALE WANG³, YUE LIU³, FENGGUO ZHAI^2,7,*

BIOCELL, Vol.47, No.7, pp. 1583-1594, 2023, DOI:10.32604/biocell.2023.028016 - 21 June 2023

Abstract Background: A preliminary miRNA screening showed that expression levels of rno-miRNA-27a-3p were significantly increased in the serum and brain tissues of rats undergoing cerebral ischemia. In recent years, there is evidence of the protective capacity of the saponins extracted from panax ginseng and its primary active ingredient ginsenosideRg1oncerebral ischemic injury. Methods: Fetal rat neurons (FRNs) were cultured in glucose-and-serum-free medium and exposed to hypoxia to establish a cerebral ischemia model in vitro (oxygen and glucose deprivation model, OGD). Antioxidant indexes (CAT, SOD), inflammatory markers (MPO, TNF-α and IL-6), and the expression of apoptosis and proliferation associated… More >

YANYA CHEN^1,#, HONGYUAN WU^2,#, RUISI ZHOU^5,#, HELING DONG⁴, XUEFANG ZHANG², XUEWEI WU¹, WENSHAN CHEN¹, YANTING YOU^5,*, YIFEN WU^3,*

BIOCELL, Vol.47, No.6, pp. 1353-1364, 2023, DOI:10.32604/biocell.2023.027346 - 19 May 2023

Abstract Background: The relationship between microRNA (miRNA) expression patterns and tumor mutation burden (TMB) in uterine corpus endometrial carcinoma (UCEC) was investigated in this study. Methods: The UCEC dataset from The Cancer Genome Atlas (TCGA) database was used to identify the miRNAs that differ in expression between high TMB and low TMB sample sets. The total sample sets were divided into a training set and a test set. TMB levels were predicted using miRNA-based signature classifiers developed by Lasso Cox regression. Test sets were used to validate the classifier. This study investigated the relationship between a miRNA-based signature… More >

Yanyan Pu^1,#, Liwen Wang^2,#, Leilei Li³, Yujun Si⁴, Shubin Xie², Yunzhe Cong¹, Dong Wang¹, Yongchao Gong¹, Rumei Tian¹, Xue Chen³, Xiaoyan Zhang¹, Min Liu¹, Hanfeng Ding^1,3,*, Nana Li^1,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1793-1812, 2023, DOI:10.32604/phyton.2023.026822 - 11 April 2023

Abstract During the life cycle of a plant, seed germination is crucial. Upon ingestion of water, the dry seeds resumed energy metabolism and cellular repair. To dissect the complex mechanisms at the very beginning of seed germination, two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed. The transcriptome sequencing analysis identified 10,108 differentially expressed genes (DEGs) between dry and imbibed mung bean seeds. KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways, carbohydrate, and energy metabolism. Out of the total… More >

Qian Zhang^1,2,#, Yuanyuan Cao^2,#, Mingming Fang³, Qin Yang², Pingyang Zhang^1,*

Oncologie, Vol.24, No.4, pp. 717-727, 2022, DOI:10.32604/oncologie.2022.026998 - 31 December 2022

Abstract Background: Breast cancer (BC) is the most common malignant tumor in women, and its morbidity and mortality are increasing each year, due to the lack of specific clinical symptoms in the early stage of BC, and the lack of diagnostic methods for early breast cancer. Therefore, identifying an effective diagnostic method for early BC has become urgent. Materials and Methods: Breast lesions with a histological diagnosis that were examined by ultrasonic elastography (UE) in our department from June 2020 to December 2021 were reviewed. qRT-PCR was performed to measure the expression levels of miR-144-5p and miR-26b-5p in… More >

MEIHUA CHEN^1,2, HUIHUI WANG³, XIAOFENG CHEN^1,2, YAN CHEN^1,2, TIANYING BIAN^1,2,*

BIOCELL, Vol.46, No.10, pp. 2281-2289, 2022, DOI:10.32604/biocell.2022.020697 - 13 June 2022

Abstract Periodontitis is a frequent chronic inflammatory disorder destroying periodontium. Recent studies have revealed the role of bromodomain and extraterminal domain inhibitor (BETi) and microRNA (miR)-130a in regulating macrophage polarization and pro-inflammatory response. However, little is known about whether apabetalone (a novel BETi) and miR-130a are correlated with chronic inflammatory state in periodontitis by regulating macrophage polarization. Here murine RAW264.7 macrophages were applied as an in vitro inflammatory model. After treatment with Porphyromonas gingivalis-derived lipopolysaccharide (Pg LPS) and apabetalone, the expression of macrophage M1 polarization markers and inflammatory cytokines was assessed using real-time PCR, western blot, and enzyme-linked… More >

BUWEN CAO^1,*, JIAWEI LUO^2,*, SAINAN XIAO^1,2, XIANGJUN ZHOU¹

BIOCELL, Vol.46, No.8, pp. 1925-1933, 2022, DOI:10.32604/biocell.2022.019613 - 22 April 2022

Abstract The association between miRNA and disease has attracted more and more attention. Until now, existing methods for identifying miRNA related disease mainly rely on top-ranked association model, which may not provide a full landscape of association between miRNA and disease. Hence there is strong need of new computational method to identify the associations from miRNA group view. In this paper, we proposed a framework, MDA-TOEPGA, to identify miRNAdisease association based on two-objective evolutionary programming genetic algorithm, which identifies latent miRNAdisease associations from the view of functional module. To understand the miRNA functional module in diseases, More >

YANG YANG, LIJUAN FU, CHUNMEI CHEN, MEIWEI HU

BIOCELL, Vol.46, No.5, pp. 1319-1328, 2022, DOI:10.32604/biocell.2022.015760 - 06 January 2022

Abstract Aberrant expression of miRNAs is significantly correlated with the occurrence of immune thrombocytopenic purpura (ITP). The immune imbalance of M1/M2 macrophage contributes to the development of ITP. However, the role of miR-148b-3p in macrophage phenotype imbalance remains unknown in ITP. In this study, we aimed to explore whether miR-148b-3p inhibits M2 macrophage polarization in ITP and to investigate the underlying mechanism. Peripheral blood from 22 ITP patients were collected, and real-time PCR confirmed that miR-148b-3p was up-regulated and Western blot analyses detected the expression of SOCS3 was down-regulated. Subsequent dual-luciferase reporter gene assay indicated that More >

HUI-YING JIN^1,2, MAO-SONG PEI^1,2, DA-LONG GUO^1,2,*

BIOCELL, Vol.46, No.4, pp. 941-949, 2022, DOI:10.32604/biocell.2022.016718 - 15 December 2021

Abstract To understand the molecular characteristics of the miR477 gene family of grape (Vvi-miR477) and to predict its target genes, the Vvi-miR477 genes were identified from previous small RNA sequencing data, then phylogenetic analysis and prediction of target gene were conducted. The Vvi-miR477 family consists of two precursor sequences and three mature sequences. The miR477 family members were mostly 19-22nt in length. The sequence is relatively conservative. Vvi-MIR477a and Vvi-MIR477b are located on chromosomes 1 and 2, respectively. These precursor sequences can form the typical stable stem-loop structure. Their minimum folding free energy is −39.10 kcal/mol and −50.90 kcal/mol, More >

BUWEN CAO^1,*, JIAWEI LUO^2,*, SAINAN XIAO¹, KAI ZHAO¹, SHULING YANG¹

BIOCELL, Vol.46, No.3, pp. 837-845, 2022, DOI:10.32604/biocell.2022.017538 - 18 November 2021

Abstract Identifying associations between microRNAs (miRNAs) and diseases is very important to understand the occurrence and development of human diseases. However, these existing methods suffer from the following limitation: first, some disease-related miRNAs are obtained from the miRNA functional similarity networks consisting of heterogeneous data sources, i.e., disease similarity, protein interaction network, gene expression. Second, little approaches infer disease-related miRNAs depending on the network topological features without the functional similarity of miRNAs. In this paper, we develop a novel model of Integrating Network Topology Similarity and MicroRNA Function Similarity (INTS-MFS). The integrated miRNA similarities are calculated… More >

RAGHDA A. SOLIMAN¹, RANA A. YOUNESS^1,2,*, TAMER M. MANIE³, EMAD KHALLAF⁴, MOHAMED EL-SHAZLY¹, MONA ABDELMOHSEN⁵, HEBA HANDOUSSA¹, MOHAMED Z. GAD^6,*

BIOCELL, Vol.46, No.3, pp. 769-783, 2022, DOI:10.32604/biocell.2022.016636 - 18 November 2021

Abstract Triple Negative Breast Cancer (TNBC) immunotherapy has recently shown promising approach. However, some TNBC patients presented with resistance. One of the reasons was attributed to the excessive release of cytokines at the tumor microenvironment (TME) such as Tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Fine regulation of these cytokines’ levels via non-coding RNAs (ncRNAs) might alleviate the immune quiescent nature of TME at TNBC tumors. However, the extrapolation of ncRNAs as therapeutic tools is highly challenging. Therefore, disentanglement the nature for the isolation of natural compounds that could modulate the ncRNAs and their respective… More >