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  • Open Access

    ARTICLE

    Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver

    CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN

    BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033

    Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 More >

  • Open Access

    ARTICLE

    Tissue expression of platelet endothelial cell adhesion molecule-1 at pre and postnatal murine development

    GRACIELA CRISTINA CALABRESE*, ROSA WAINSTOK**

    BIOCELL, Vol.28, No.3, pp. 251-258, 2004, DOI:10.32604/biocell.2004.28.251

    Abstract Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages.
    Fluorescence activated cell sorter analysis showed a different… More >

  • Open Access

    ARTICLE

    Adjuvant effects of Lactobacillus casei added to a renutrition diet in a malnourished mouse model

    PAOLA GAUFFIN CANO1 , GRACIELA AGÜERO3, GABRIELA PERDIGON1,2

    BIOCELL, Vol.26, No.1, pp. 35-48, 2002, DOI:10.32604/biocell.2002.26.035

    Abstract Nutritional deficiencies are associated with impaired immune response, affecting the body’s defence mechanisms. It is also known that Lactic Acid Bacteria (LAB) and fermented products such us yogurt have immunopotentiator activity and nutritional properties, and could thus be used as a valuable supplement in a renutrition diet. The aim of this study was to determine, in a non-severe malnutrition model, the effective dose of Lactobacillus casei (L.casei), which when is used as an adjuvant in a renutrition diet, would modulate the mucosal immune system and induce recovery of the integrity of the intestinal barrier. The experiments… More >

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