Orlando J. CASTEJÓN^*

BIOCELL, Vol.39, No.2-3, pp. 33-40, 2015, DOI:10.32604/biocell.2015.39.033

Abstract Twenty one days old Swiss albino mice that received diphenylhydantoin (25 mg/kg, i.p., daily for 15 days) progressively developed gait alterations, changes of behavior and cerebellar ataxia. Cerebellar slices were processed by conventional transmission electron microscopy. The body of Purkinje cells exhibited fragmented limiting plasma membranes, dilated nuclear envelopes, swelling and disassembly of nuclear pores, enlargement of rough and smooth endoplasmic reticulum and a notable detachment of membrane associated ribosomes, together with distorted vacuoles of smooth endoplasmic reticulum, bizarre shaped and swollen mitochondria with dilated cristae, as well as disrupted limiting lysosomal membranes. Degenerated… More >

Ozbey G¹, Gorczynski R², Erin N¹

European Cytokine Network, Vol.25, No.2, pp. 30-34, 2014, DOI:10.1684/ecn.2014.0353

Abstract Measurements of cytokines in cell culture supernatants are widely used to evaluate the immune response. Cytokine levels in secretomes are usually quantified using enzyme-linked immunosorbent assays (ELISA), which have easy, sensitive, specific, rapid, cost-effective, and reproducible protocols. To our knowledge, the stability of cytokines in secretomes has not been hitherto investigated. We present data that involve; time-dependent changes during storage at +4℃, and the effects of freeze-thaw cycles in samples frozen at -80℃, instant freezing of samples with liquid nitrogen, and addition of protease inhibitors on the stability of certain cytokines (TNF-α, MIP-2, IFN-γ, IL-6, More >

Norizadeh Abbariki Tannaz^*,†, Shokrgozar Mohammad Ali^†,‡, Haghighipour Nooshin^*,§, Aghdami Nasser^¶, Mahdian Rezak^II, Amanzadeh Amir^*, Jazayeri Maryam^*,†

Molecular & Cellular Biomechanics, Vol.11, No.1, pp. 19-37, 2014, DOI:10.3970/mcb.2014.011.019

Abstract Background: Environmental factors affect stem cell differentiation. In addition to chemical factors, mechanical signals have been suggested to enhance myogenic differentiation of stem cells. Therefore, this study was undertaken to illustrate and compare the effect of chemical and mechanical stimuli on Myogenin (MyoG) and Myosin heavy chani 2 (Myh2) expression of mouse bone marrowderived mesenchymal stem cells (BMSCs) and embryonic stem cells (ESCs). Methods: After isolation and expansion of BMSCs and generation of embryoid bodies and spontaneous differentiation of ESCs, cells were examined in 4 groups: (1) control group: untreated cells; (2) chemical group: cells… More >

Astrid Stienen^1,2, Oliver Feyen¹, Tim Niehues^1,3

European Cytokine Network, Vol.22, No.1, pp. 63-72, 2011, DOI:10.1684/ecn.2011.0273

Abstract Interleukin (IL)-7 is thought to be a non-redundant cytokine for lymphopoiesis as there is a reduction of T and B cells in peripheral blood (PB) and a loss of TCRγδ⁺ cells in PB and bone marrow (BM) in IL-7^-/- mice. To investigate whether the absence of IL-7 influences the organ-dependent distribution of the lymphocytes, we analyzed single cell suspensions of several organs (BM, lung, liver, small intestine, and spleen) at different ages (three and 12 months) of IL-7^+/+ and IL-7^-/- mice using flow cytometry; immunohistochemical staining was performed on frozen sections of various organs. We observed lymphocytopenia… More >

ALI M.SHARIFI^1,2*, RADBOD DARABI², AND KHOSROW JADIDI³

BIOCELL, Vol.34, No.1, pp. 53-55, 2010, DOI:10.32604/biocell.2010.34.053

Abstract The effects of human versus mouse EGF on cell growth and culture duration were studied to optimize a human limbal stem cells culture method for therapeutical autologous transplantation. Limbal cells were obtained by trypsin digestion and transferred to a culture medium. The time needed to reach full confluence in culture was determined. Specific antibodies to corneal stem cell marker (P63) versus corneal epithelial differentiation marker (K3) were used for histochemical determinations. A high proportion of P63 positive cells (85± 4.6%), and a correspondingly low proportion K3 positive cells (15 ± 3.8%) indicated that most cultured More >

Anna M. Raskin^∗,†, Masahiko Hoshijima^†, Eric Swanson^†, Andrew D. McCulloch^*, Jeffrey H. Omens^∗,†,‡

Molecular & Cellular Biomechanics, Vol.6, No.3, pp. 145-160, 2009, DOI:10.3970/mcb.2009.006.145

Abstract Altered mechanical stress and strain in cardiac myocytes induce modifications in gene expression that affects cardiac remodeling and myocyte contractile function. To study the mechanisms of mechanotransduction in cardiomyocytes, probing alterations in mechanics and gene expression has been an effective strategy. However, previous studies are self-limited due to the general use of isolated neonatal rodent myocytes or intact animals. The main goal of this study was to develop a novel tissue culture chamber system for mouse myocardium that facilitates loading of cardiac tissue, while measuring tissue stress and deformation within a physiological environment. Intact mouse… More >

SILVIA S. BORKOSKY^1,2, HITOSHI NAGATSUKA¹, YORIHISA ORITA³, HIDETSUGU TSUJIGIWA⁴, JUNKO YOSHINOBU³, MEHMET GUNDUZ¹, ANDREA P. RODRIGUEZ¹, LILIANA R. MISSANA², KAZUNORI NISHIZAKI³, NORIYUKI NAGAI¹

BIOCELL, Vol.32, No.3, pp. 251-257, 2008, DOI:10.32604/biocell.2008.32.251

Abstract The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15… More >

ALICIA WEYERS, LAURA I. UGNIA, HUGO GARCÍA OVANDO, NORA B. GORLA*

BIOCELL, Vol.32, No.1, pp. 27-31, 2008, DOI:10.32604/biocell.2008.32.027

Abstract In the present study, the antioxidant capacity of vitamin C was examined in the liver and the kidney tissues of mice with or without ciprofloxacin (CFX) treatment. The antioxidant capacity of the vitamin was evaluated in terms of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARs). The experimental design was 15 days of water (control and CFX groups) or vitamin C (vitamin C and vitamin C plus CFX groups) in drinking water. One dose of CFX was injected, 15 minutes before sacrifice, in the corresponding mice.
The initial nmol of lipid hydroperoxides/g of tissue… More >

VANINA L. JULIANELLI¹ , LILIANA N. GUERRA^*1, JUAN C. CALVO^*1,2

BIOCELL, Vol.31, No.2, pp. 237-245, 2007, DOI:10.32604/biocell.2007.31.237

Abstract Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found More >

ELISA CEBRAL^1,2, ISABEL CARRASCO¹ , DAVID VANTMAN¹, ROSITA SMITH¹

BIOCELL, Vol.31, No.1, pp. 51-59, 2007, DOI:10.32604/biocell.2007.31.051

Abstract Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H₂O₂) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H₂O₂ for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared… More >