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  • Open Access

    REVIEW

    Role of Pathogen-Related Protein 10 (PR 10) under Abiotic and Biotic Stresses in Plants

    Rakesh Kumar Sinha1,*, Shiv Shankar Verma2, Anshu Rastogi3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 167-182, 2020, DOI:10.32604/phyton.2020.09359 - 22 April 2020

    Abstract Members of the Pathogenesis Related (PR) 10 protein family have been identified in a variety of plant species and a wide range of functions ranging from defense to growth and development has been attributed to them. PR10 protein possesses ribonuclease (RNase) activity, interacts with phytohormones, involved in hormone-mediated signalling, afforded protection against various phytopathogenic fungi, bacteria, and viruses particularly in response to biotic and abiotic stresses. The resistance mechanism of PR10 protein may include activation of defense signalling pathways through possible interacting proteins involved in mediating responses to pathogens, degradation of RNA of the invading… More >

  • Open Access

    REVIEW

    Role of pyroptotic cell death in the pathogenesis of NASH

    Yuguo YI, Jiamin ZHENG, Yang ZHOU, Zhiqin LIU, Dan WENG*

    BIOCELL, Vol.44, No.1, pp. 7-11, 2020, DOI:10.32604/biocell.2020.08686 - 01 March 2020

    Abstract Nonalcoholic fatty liver disease (NAFLD) represents a huge threat to public health of the whole world. Around 25% of NAFLD patients will progress to nonalcoholic steatohepatitis (NASH), which has been predicted to be the main reason for liver transplantation in the United States in 2020. Extensive effort has been devoted to investigating the underlying molecular mechanisms of NASH pathogenesis and developing new promising treatments. Recent studies have demonstrated that pyroptosis, an inflammatory programmed cell death mediated by inflammasome and gasdermin-D (GSDMD), is involved in the development and progression of NASH. This review aims to summarize More >

  • Open Access

    ARTICLE

    Green Synthesis of Silver Nanoparticles from Abronia villosa as an Alternative to Control of Pathogenic Microorganisms

    Ali Abdelmoteleb1, Benjamin Valdez-Salas2, Ernesto Beltran-Partida2, Daniel Gonzalez-Mendoza3,*

    Journal of Renewable Materials, Vol.8, No.1, pp. 69-78, 2020, DOI:10.32604/jrm.2020.08334 - 01 January 2020

    Abstract The aim of this study was to evaluate the antibacterial and antifungal activities of eco-friendly synthesized silver nanoparticles. The silver nanoparticles were synthesized by biological method using aqueous extract of Abronia villosa. Synthesis of silver nanoparticles was confirmed by color change and characterized using UV-visible spectroscopy, scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDX), dynamic light scattering (DLS), and zeta potential analysis. The SEM analysis revealed the presence of spherical silver nanoparticles of the size range 21 to 33 nm. Synthesized silver nanoparticles were used to evaluate their antibacterial effects at different concentrations (25, 50, More >

  • Open Access

    ARTICLE

    In vitro evaluation of native entomopathogenic fungi and neem (Azadiractha indica) extracts on Spodoptera frugiperda

    Hernandez-Trejo A1, B Estrada-Drouaillet1, JA López-Santillán1, C Rios-Velasco2, SE Varela-Fuentes1, R Rodríguez-Herrera3, E Osorio-Hernández1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 47-54, 2019, DOI:10.32604/phyton.2019.04611

    Abstract The control of Spodoptera frugiperda is based on synthetic insecticides, so some alternatives are the use of entomopathogenic fungi (EF) and neem extract. The objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on S. frugiperda larvae. Six EF were identified by DNA sequencing of ITS regions from three EF (Fusarium solani, Metarrhizium robertsii, Nigrospora spherica and Penicillium citrinum). They were evaluated in concentrations of 1 × 10⁸ spores/ mL. In addition, a second bioassay was carried out evaluating only F. solani, M. robertsii and N. sphaerica and… More >

  • Open Access

    ARTICLE

    First report of Phytopythium vexans causing the “Avocado sadness” in Michoacan, Mexico

    Hernández Pérez A1, E Cerna Chávez2, JC Delgado Ortiz3, M Beltrán Beache4, LM Tapia Vargas5, YM Ochoa Fuentes2

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 11-13, 2019, DOI:10.32604/phyton.2019.04608

    Abstract Mexico is the main producer, consumer and exporter of avocado in the world, being Michoacan the main producer state contributing more than 80% of the national production. There are phytopathogens that decimate the production causing the death of the tree. Root samples were collected in avocado trees that showed the characteristic symptomatology of the disease known as avocado sadness, the sampling was carried out in four of the main avocado producing towns, in the state of Michoacan, Mexico. The isolation consisted in sowing root tissue in Petri dishes with V8®-PARPH culture medium, subsequently they were identified More >

  • Open Access

    ARTICLE

    Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study

    Juan Agustín CUETO3, Emile SANTOS BARRIAS6, Wanderley de SOUZA4, 5, Patricia Silvia ROMANO1, 2

    BIOCELL, Vol.42, No.3, pp. 105-108, 2018, DOI:10.32604/biocell.2018.07017

    Abstract Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed More >

  • Open Access

    ARTICLE

    Hydrothermal preparation of TiO2-Ag nanoparticles and its antimicrobial performance against human pathogenic microbial cells in water

    Mahmoud MOUSTAFA1, 2, Saad ALAMRI1, Mohamed ELNOUBY3, Tarek TAHA4, M. A. ABU-SAIED5, Ali SHATI1, Mohamed AL-KAHTANI1, Sulaiman ALRUMMAN1

    BIOCELL, Vol.42, No.3, pp. 93-98, 2018, DOI:10.32604/biocell.2018.07014

    Abstract Water contaminated with pathogenic microbes is considered as one of the most common routes for transmitting diseases in human beings. Different methods have been applied for the decontamination of microbes in contaminated water. In the current study, an easy to do hydrothermal method has been used for the preparation of TiO2-Ag nanoparticles. The obtained material was characterised using a scanning electron microscope (SEM) and fourier transform infra-red spectroscopy (FTIR). The morphological appearance of the obtained nanoparticles was in the shape of a sphere with a size range of 60-90 nm. The antimicrobial activity of the prepared More >

  • Open Access

    ARTICLE

    Whole exome sequencing with genomic triangulation implicates CDH2-encoded N-cadherin as a novel pathogenic substrate for arrhythmogenic cardiomyopathy

    Kari L. Turkowski1, David J. Tester2,3, J. Martijn Bos2,4, Kristina H. Haugaa5, Michael J. Ackerman2,3,4

    Congenital Heart Disease, Vol.12, No.2, pp. 226-235, 2017, DOI:10.1111/chd.12462

    Abstract Background: Arrhythmogenic cardiomyopathy (ACM) is a heritable disease characterized by fibrofatty replacement of cardiomyocytes, has a prevalence of approximately 1 in 5000 individuals, and accounts for approximately 20% of sudden cardiac death in the young (≤35 years). ACM is most often inherited as an autosomal dominant trait with incomplete penetrance and variable expression. While mutations in several genes that encode key desmosomal proteins underlie about half of all ACM, the remainder is elusive genetically. Objective: Here, whole exome sequencing (WES) was performed with genomic triangulation in an effort to identify a novel explanation for a… More >

  • Open Access

    ARTICLE

    Effect of phenolic compounds present in Argentinian plant extracts on mycelial growth of the plant pathogen Botrytis cinerea Pers.

    Hapon MV1,2, JJ Boiteux1,2, MA Fernández1, G Lucero1,2, MF Silva1, PH Pizzuolo1,2

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 270-277, 2017, DOI:10.32604/phyton.2017.86.270

    Abstract The polyphagous plant pathogen Botrytis cinerea has the ability of growing fast under several conditions, and producing grey moulds in different plant tissues. Because of its adaptive plasticity, resistant strains have made difficult their chemical control. Therefore, new strategies of management and the use of natural compounds could be important control alternatives. The aim of this work was to study the potential value of four Argentine native plant extracts and some of its phenolic compounds as biodrugs on B. cinerea. Aqueous plant extracts of Larrea divaricata, Prosopis strombulifera, Tessaria absinthioides and Schinus molle var. areira, were used. The More >

  • Open Access

    ARTICLE

    In vitro antifungal/fungistatic activity of manganese phosphite against soybean soil-borne pathogens

    Carmona MA1, E Simonetti2,3, ME Ravotti1, MM Sc,iani4, AG Luque4, NA Formento5, FJ Sautua1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 265-269, 2017, DOI:10.32604/phyton.2017.86.265

    Abstract Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotinia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar media (25 mL) supplemented with different concentrations of manganese phosphite (MnPhi) diluted in water (0, 25, 37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelial growth sensitivity was analyzed using logarithmic linear regression analysis. The MnPhi concentration needed to inhibit 50% of the mycelial growth (IC50) More >

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