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  • Open Access


    In vitro antagonistic activity of Bacillus subtilis strains isolated from soils of the Yucatan Peninsula against Macrophomina phaseolina and Meloidogyne incognita

    Ruiz SE, AJ Cristóbal, RA Reyes, SJ Tun, RA García, AJ Pacheco

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 45-47, 2014, DOI:10.32604/phyton.2014.83.045

    Abstract The antagonistic activity of native Bacillus subtilis strains from Yucatán peninsula soils were evaluated on two soilborne pathogens. Bacillus subtilis cbck36 and cbrf24 caused more than 60% inhibition of colony growth in Machophomina phaseolina. Cell-free culture filtrate of B. subtilis cbr24 were active against second- stage juveniles (J2) of Meloidogyne incognita (LC50 25.8% v/v). More >

  • Open Access


    In vitro biocontrol of tomato pathogens using antagonists isolated from chicken-manure vermicompost

    Barocio-Ceja NB1, LF Ceja-Torres1*, JL Morales-García2, HV Silva-Rojas3, R Flores-Magallón1, S Ochoa-Estradav1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 15-22, 2013, DOI:10.32604/phyton.2013.82.015

    Abstract The objectives of this study were to (1) isolate and identify pathogenic fungi from vegetative material with wilt symptoms in tomato plantations belonging to the Cienega of Chapala, Michoacán, Mexico, and (2) determine the antagonistic capacity of Trichoderma sp. and Aspergillus spp. isolated from chicken-manure vermicompost. Pathogens were isolated by means of a completely randomized sampling in 6 locations; 9 plantations were inspected and 45 plants with symptoms of the disease were selected. Portions of root and stem were disinfected and placed on potato-dextroseagar acidified (PDA). Antagonists isolation was made from a dilution of chicken-manure vermicompost of 1 x 10-2More >

  • Open Access


    Antibacterial activity of Falkenbergia hillebrandii (Born) from the Indian coast against human pathogens

    Manilal1 A, S Sujith2, J Selvin3, C Shakir4, G Seghal Kiran5

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 161-166, 2009, DOI:10.32604/phyton.2009.78.161

    Abstract The antibacterial property of the red algae, Falkenbergia hillebrandii (Born) collected from the southwest coast of India (Indian Ocean) was evaluated against three multidrug resistant human pathogens. Four different solvents: ethyl acetate, dichloromethane, methanol and phosphate buffer saline (PBS) were used with this purpose. Dried samples extracted with methanol showed broadest and highest antimicrobial activity when compared to other solvents. However, PBS extract showed no antibacterial activity. The highly active compounds red alga, F. hillebrandii were fractionated and purified using different chromatographic systems, including reverse phase HPLC and GCMS. The analysis revealed that the most abundant metabolite was the oleic… More >

  • Open Access


    Biological control of chili pepper root rot (Capsicum annuum L.) by Bacillus thuringiensis

    Mojica-Marín1* V, HA Luna-Olvera2, CF Sandoval-Coronado2, B Pereyra-Alférez2, LH Morales-Ramos2, NA González-Aguilar2, CE Hernández-Luna2, OG Alvarado-Gomez3

    Phyton-International Journal of Experimental Botany, Vol.78, pp. 105-110, 2009, DOI:10.32604/phyton.2009.78.105

    Abstract Wide spreading of pepper blight on new plantations and on different production areas in Mexico, and the difficulty in controlling this disease, makes it compulsory to develop new control measures. It is expected that these measures provide new tools for controlling this disease in the context of integrated management strategies. The aim of this investigation was to evaluate and determine the antagonistic potential of 64 strains of Bacillus thuringiensis against Rhizoctonia solani, Phytophthora capsici and Fusarium oxysporum by dual culture assays. Strains of B. thuringiensis came from the International Collection of Entomopatogenic Bacillus from FCBUANL. Results showed that 16 strains… More >

  • Open Access


    Diagnosis of the microbiological quality of fruits and vegetables in Chihuahua, Mexico

    Ávila-Quezada1* G, E Sánchez1, E Muñoz1, LR Martínez2, E Villalobos2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 129-136, 2008, DOI:10.32604/phyton.2008.77.129

    Abstract We determined the microbiological quality of the most important fruits and vegetables in Chihuahua State (Mexico) to know the potential risks and apply prevention strategies prior to their consumption. Samples of jalapeño, bell and serrano pepper, cantaloupe, saladet and grape tomato, peach and Golden and Starkimson apple and of well, channel, irrigation and drinking water, as well as that for washing in packing were analyzed to determine Salmonella spp., Escherichia coli, total and fecal coliforms, fungi and yeasts. Determinations were made following the methodology indicated by the Mexican regulations (NOM-111-SSA1-1994). Sampling was made in the whole Chihuahua State during the… More >

  • Open Access


    Effect of phenolic compounds present in Argentinian plant extracts on mycelial growth of the plant pathogen Botrytis cinerea Pers.

    Hapon MV1,2, JJ Boiteux1,2, MA Fernández1, G Lucero1,2, MF Silva1, PH Pizzuolo1,2

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 270-277, 2017, DOI:10.32604/phyton.2017.86.270

    Abstract The polyphagous plant pathogen Botrytis cinerea has the ability of growing fast under several conditions, and producing grey moulds in different plant tissues. Because of its adaptive plasticity, resistant strains have made difficult their chemical control. Therefore, new strategies of management and the use of natural compounds could be important control alternatives. The aim of this work was to study the potential value of four Argentine native plant extracts and some of its phenolic compounds as biodrugs on B. cinerea. Aqueous plant extracts of Larrea divaricata, Prosopis strombulifera, Tessaria absinthioides and Schinus molle var. areira, were used. The capability to… More >

  • Open Access


    In vitro antifungal/fungistatic activity of manganese phosphite against soybean soil-borne pathogens

    Carmona MA1, E Simonetti2,3, ME Ravotti1, MM Sc,iani4, AG Luque4, NA Formento5, FJ Sautua1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 265-269, 2017, DOI:10.32604/phyton.2017.86.265

    Abstract Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotinia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar media (25 mL) supplemented with different concentrations of manganese phosphite (MnPhi) diluted in water (0, 25, 37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelial growth sensitivity was analyzed using logarithmic linear regression analysis. The MnPhi concentration needed to inhibit 50% of the mycelial growth (IC50) ranged from… More >

  • Open Access


    Morphological, molecular and pathogenic diagnostic of fungi in lawn’s root and neck in Mexico

    García-Munguía CA1, M Acosta-Ramos2, O Vázquez-Martinez3, M Pérez-Sato5, A García-Munguía4, O García-Munguía6, AM García-Munguía3

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 258-264, 2017, DOI:10.32604/phyton.2017.86.258

    Abstract The illnesses that cause root and neck rot in golf courses constitute a limiting factor for grass development worldwide. This is mainly true when management guidelines are not conducted. Our research was conducted in the Golf Club “Mexico”, located in Mexico, D.F. Isolates were taken from plants that presented yellowing symptoms and root and neck rot. The identified pathogenic fungi, isolated from axenic media, were Giberella avenacea, Fusarium oxysporum, F. oxysporum f. sp. cubense and Pythium ultimum. The molecular characterization showed a comparison of the DNA-obtained sequences which was morphologically determined as F. avenaceum and was molecularly corroborated as G.… More >

  • Open Access


    Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study

    Juan Agustín CUETO3, Emile SANTOS BARRIAS6, Wanderley de SOUZA4, 5, Patricia Silvia ROMANO1, 2

    BIOCELL, Vol.42, No.3, pp. 105-108, 2018, DOI:10.32604/biocell.2018.07017

    Abstract Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed by a… More >

  • Open Access


    Hydrothermal preparation of TiO2-Ag nanoparticles and its antimicrobial performance against human pathogenic microbial cells in water

    Mahmoud MOUSTAFA1, 2, Saad ALAMRI1, Mohamed ELNOUBY3, Tarek TAHA4, M. A. ABU-SAIED5, Ali SHATI1, Mohamed AL-KAHTANI1, Sulaiman ALRUMMAN1

    BIOCELL, Vol.42, No.3, pp. 93-98, 2018, DOI:10.32604/biocell.2018.07014

    Abstract Water contaminated with pathogenic microbes is considered as one of the most common routes for transmitting diseases in human beings. Different methods have been applied for the decontamination of microbes in contaminated water. In the current study, an easy to do hydrothermal method has been used for the preparation of TiO2-Ag nanoparticles. The obtained material was characterised using a scanning electron microscope (SEM) and fourier transform infra-red spectroscopy (FTIR). The morphological appearance of the obtained nanoparticles was in the shape of a sphere with a size range of 60-90 nm. The antimicrobial activity of the prepared nanoparticles was tested against… More >

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