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  • Open Access

    ARTICLE

    Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment

    MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO S.TRIPPI

    BIOCELL, Vol.26, No.2, pp. 217-223, 2002, DOI:10.32604/biocell.2002.26.217

    Abstract Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other More >

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  • Open Access

    ARTICLE

    Micropropagation of Prosopis chilensis (Mol.) Stuntz from young and mature plants

    L.A. CARO1, P.A. POLCI1 , L.I. LINDSTRÖM1 , C.V. ECHENIQUE1,2, L.F. HERNÁNDEZ1,3

    BIOCELL, Vol.26, No.1, pp. 25-33, 2002, DOI:10.32604/biocell.2002.26.025

    Abstract Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 – 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm,… More >

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    7

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