Sukhraj Kaur, Harsimrat Kaur, Prati Pal Singh

European Cytokine Network, Vol.15, No.4, pp. 327-338, 2004

Abstract Colony-stimulating factors (CSFs)-induced increased hematopoietic activity is known to occur in various microbial diseases; however, not much is known during tuberculosis (TB). We investigated the CSF-inducing capability of a Mycobacterium tuberculosis H37Rv component. Swiss mice intravenously injected with purified 30-kDa secretory protein of M. tuberculosis H37Rv (Mtb30; 0.1-10 mg/kg) showed enhanced levels of serum CSFs; maximum response (142 ± 16 colonies) occurred at 1 mg/kg. In vitro, Mtb30 (1-50 lg/mL) induced mouse peritoneal macrophages (PMs) to elaborate CSFs in the conditioned medium (CM); 25 lg/mL appeared optimal (97 ± 11 colonies). Both in vivo and in vitro,… More >

Uzair B. Chaudhary¹, David L. Milling¹, Nabil K. Bissada²

Canadian Journal of Urology, Vol.11, No.1, pp. 2136-2138, 2004

Abstract Hypercalcemia associated with transitional cell carcinoma (TCC) is rarely encountered. We report a case of TCC of bladder with documented production of parathyroid hormone related protein (PTHrP). Our patient had a rapidly progressive course and died 2 months after radical cystectomy. Literature suggests that these patients present with advanced stage and carry a poor prognosis. The histopathologic features, treatment and prognosis associated with this rare paraneoplastic syndrome are reviewed. More >

KARINA MANCINI, HEIDI DOLDER

BIOCELL, Vol.28, No.3, pp. 299-310, 2004, DOI:10.32604/biocell.2004.28.299

Abstract This study was undertaken to detect protein components in both sperm types of the butterfly Euptoieta hegesia. These spermatozoa possess complex extracellular structures for which the composition and functional significance are still unclear. In the apyrene sperm head, the proteic cap presented an external ring and an internal dense content; basic proteins were detected only in external portions. In the tail, the paracrystalline core of mitochondrial derivatives and the axoneme are rich in proteins. The extratesticular spermatozoa are covered by a proteic coat, which presented two distinct layers. In eupyrene spermatozoa, acrosome and nucleus were negatively… More >

Min Yue^1,1, Jeanne C. Stachowiak^1,1,2,2, Arunava Majumdar^1,1,3,3

Molecular & Cellular Biomechanics, Vol.1, No.3, pp. 211-220, 2004, DOI:10.3970/mcb.2004.001.211

Abstract Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in More >

Séverine Froget¹, Emmanuelle Barthelemy², François Guillot¹, Christophe Soler¹, Marie-Claude Coudert², Marc Benbunan², Christine Dosquet^2,3

European Cytokine Network, Vol.14, No.1, pp. 60-64, 2003

Abstract Cell and tissue therapy applications in humans are being used increasingly, particularly for tissue repair. Several reconstructed skin models have been proposed. Wound healing involves overlapping steps of inflammation, cell migration and proliferation, neovascularisation, extracellular matrix production and remo-delling. This is regulated by numerous cytokines and other soluble mediators. We have prepared dermal substitutes (DS) consisting of a collagen-GAG, three-dimensional matrix colonized by human dermal fibroblasts (HDF), isolated by skin explant or enzymatic digestion of the skin for potential therapeutic use in humans. To test the functionality of these DS, we measured (ELISA) the stimulatory… More >

Vincent L. Butty¹, Pascale Roux-Lombard², Jorge Garbino³, Jean-Michel Dayer², Bara Ricou¹, and the Geneva Sepsis Network¹ (participating members are listed in the appendix)

European Cytokine Network, Vol.14, No.1, pp. 15-19, 2003

Abstract Objectives: To investigate the effects of Lenercept®, a recombinant soluble TNF receptor p55 fused to an immunoglobulin heavy chain IgG₁, on the balance of pro- and anti-inflammatory mediators in sepsis.
Design: Post hoc analysis of a subgroup of patients enrolled in a multicenter phase III, prospective, double-blind, placebo-controlled, randomized study of Lenercept ® in severe sepsis.
Setting: Surgical and medical intensive care units, and postoperative recovery room of a tertiary care teaching hospital.
Patients: A total of 57 patients were enrolled in the multicenter study in our center.
Intervention: Septic patients were randomly assigned to receive either Lenercept… More >

John E. Sims, Dirk E. Smith

European Cytokine Network, Vol.14, No.2, pp. 77-81, 2003

Abstract This article has no abstract. More >

Carita Eklund¹, Farhana Jahan¹, Tanja Pessi¹, Terho Lehtimäki², Mikko Hurme^1,3

European Cytokine Network, Vol.14, No.3, pp. 168-171, 2003

Abstract C-reactive protein (CRP) is a sensitive marker of inflammation induced by both IL-6 and IL-1. Thus, genetic variation in these genes could be associated with the variety in C-reactive protein levels, and therefore with the severity of the entire inflammatory response. Even a subtle elevation in baseline CRP levels in healthy individuals has been found to significantly increase the risk for cardiovascular diseases. Therefore, to find out the possible role of pro-inflammatory cytokines in CRP baseline regulation we conducted a study of 338 healthy blood donors whose CRP levels were determined and whose single nucleotide More >

Dariusz P. Olszyna^1,2, Annelies Verbon², John P. Pribble³, Terence Turner³, Tim Axtelle³, Sander J. H. van Deventer¹, Tom van der Poll^1,2

European Cytokine Network, Vol.14, No.3, pp. 158-162, 2003

Abstract To determine the role of CD14 in lipopolysaccharide (LPS)-induced release of chemokines, 16 humans were injected with LPS (4 ng/kg) preceded (– 2 h) by intravenous IC14, an anti-human CD14 monoclonal antibody, or placebo. LPS elicited increases in interleukin (IL)-8 concentrations in plasma and in lysates of red blood cell (RBC), polymorphonuclear cell and mononuclear cell fractions, which were all reduced by IC14. LPS also induced rises in the plasma and RBC levels of monocyte chemoattractant protein (MCP)-1, which were diminished by IC14. Macrophage inflammatory protein (MIP)-1a and MIP-1β, chemokines that in contrast to IL-8 More >

BIOCELL, Vol.27, Suppl.S, pp. 1-172, 2003

Abstract This article has no abstract. More >