Gang Li1, Yao Zhou2, Yaqi Zhao2, Yaxue Liu2, Yuwei Ke2, Xiaoqing Jin1, Haoli Ma1,2,*
Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 329-344, 2020, DOI:10.32604/phyton.2020.08874
Abstract Quantitative real-time PCR (qRT-PCR) is widely used for investigating
gene expression patterns and has many advantages, including its high sensitivity,
fidelity, and specificity. Selecting a satisfactory internal reference gene is crucial
for obtaining precise gene expression results in qRT-PCR analyses. In this study,
the transcriptomic data of 2 potato varieties were screened for housekeeping genes
with stable expression patterns. A total of 77 putative genes were selected, which
were highly and stably expressed. Then, qRT-PCR analyses were performed to
examine the expression levels of these 77 candidate reference genes in various
potato tissues, including leaves, flowers, stolons, and tubers. Gene… More >
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