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  • Open Access

    ARTICLE

    High-Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs May Be Involved in Pectinesterase-Mediated Cold Resistance in Potato

    Chongchong Yan1, Qianqian Wang1, Nan Zhang2, Jiajia Wang1, Xuexiang Ren1, Bingjie Xue3, Xiaojing Pu4, Zhihong Xu4, Huajun Liao1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 561-586, 2020, DOI:10.32604/phyton.2020.010322

    Abstract Since potato cultivars are sensitive to low temperature, cold injury severely affects the geographical distribution and yield of potato. Although some miRNAs have been identified in response to cold stress in plants, there is no report about the role of miRNAs in the response to cold stress in potato. Here, via high throughput sequencing, we described the profiling of cold stress response to miRNA and mRNA in potato. Two small RNA and six mRNA libraries were constructed and sequenced. 296 known and 211 novel miRNAs were identified, in which 34 miRNAs in Cold Group (CG) had the higher expression quantity… More >

  • Open Access

    ARTICLE

    Detection of new antibiotic resistance gene profile in Escherichia coli associated with avian leukosis virus infection from broiler chickens

    HAIFENG WANG1,2,*, JUAN GUO1, LIJING ZHENG1, SHUYING LIU1, ZHERONG WANG1, HONGXUAN HE2

    BIOCELL, Vol.44, No.2, pp. 217-224, 2020, DOI:10.32604/biocell.2020.09051

    Abstract The Escherichia coli (E. coli) is prevailing worldwide, but the epidemiology of E. coli infections feature regional distribution characteristics to some extent. E. coli, as a zoonotic pathogen, can be transferred from animals to humans through food chain or via contact with wounds, causing a public health risk. We reported the swelling of proventriculus and tracheal bleeding following the death in two broiler chickens (Gallus gallus domesticus) from Beijing, China. To investigate whether a virus was involved in the infection, Madin Darby Bovine Kidney (MDCK) cells were co-cultured with supernatants of proventriculus, trachea and spleen homogenates. The avian leucosis virus… More >

  • Open Access

    ARTICLE

    Analysis of Water Transport inside a Plant Xylem Vessel with Pitted Thickening

    Tianyu Xu, Lixiang Zhang*

    FDMP-Fluid Dynamics & Materials Processing, Vol.16, No.3, pp. 525-536, 2020, DOI:10.32604/fdmp.2020.09618

    Abstract In this article, computational fluid dynamics (CFD) are used to explore the dynamics of water transport inside the pitted thickening of a plant xylem vessel. A pitted thickening model combined with the Bernoulli equation is used to analyze the influence of various factors (namely, the inner diameter, thickening width, thickening height, thickening spacing, number of laps and adjacent pit axial rotation). The pressure drop and the flow resistance coefficient are the variable parameters for our analysis. The results show that these two parameters are proportional to the thickening height and thickening width, and inversely proportional to the inner diameter, thickening… More >

  • Open Access

    ARTICLE

    Hemodynamic effects of ketamine in children with congenital heart disease and/or pulmonary hypertension

    Rohit S. Loomba1, Seth B. Gray2, Saul Flores3

    Congenital Heart Disease, Vol.13, No.5, pp. 646-654, 2018, DOI:10.1111/chd.12662

    Abstract Introduction: Ketamine is a drug often used for procedural sedation or as adjunct agent for general sedation in children with congenital heart disease. In the clinical realm, there is often confusion regarding the effects of ketamine on hemodynamics, particularly pulmonary vascular resistance and systemic vascular resistance. We per‐ formed a meta‐analysis of studies investigating the effects of ketamine on hemodynamics.
    Methods: A systematic review was conducted to identify studies characterizing the hemodynamic effects of ketamine in children with congenital heart disease. Studies were assessed for quality and those of satisfactory quality with pre‐ and postketa‐ mine hemodynamics for each patient… More >

  • Open Access

    ARTICLE

    Effect of Patent Ductus Arteriosus on Pulmonary Vascular Disease

    Ranjit Philip1, Jason Nathaniel Johnson1,2, Ronak Naik1, Dai Kimura1,3, Umar Boston1, Sandeep Chilakala1, Benjamin Hendrickson1, Benjamin Rush Waller1, Shyam Sathanandam1

    Congenital Heart Disease, Vol.14, No.1, pp. 37-41, 2019, DOI:10.1111/chd.12702

    Abstract The hemodynamic effects of a patent ductus arteriosus (PDA) are well known including systemic hypoperfusion and volume overload on the left ventricle. This article aims to provide a review of the long-standing effect of a hemodynamically significant PDA on the pulmonary vasculature and the role of cardiac catheterization in preterm infants with a PDA and pulmonary hypertension. More >

  • Open Access

    ARTICLE

    Vegetative Compatibility and Virulence Diversity of Verticillium dahliae from Okra (Abelmoschus esculentus) Plantations in Turkey and Evaluation of Okra Landraces for Resistance to V. dahliae

    Fatih M. Tok1, Sibel Dervis2,*, Halit Yetisir3

    Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 303-314, 2020, DOI:10.32604/phyton.2020.08801

    Abstract Forty-four V. dahliae isolates were collected from symptomatic vascular tissues of okra plants each from a different field in eight provinces located in the eastern Mediterranean and western Anatolia regions of Turkey during 2006- 2009. Nitrate-nonutilizing (nit) mutants of V. dahliae from okra were used to determine heterokaryosis and genetic relatedness among isolates. All isolates from okra plants were grouped into two vegetative compatibility groups (VCGs) (1 and 2) and three subgroups as 1A (13.6%, 6/44), 2A (20.5%, 9/44) and 2B (65.9%, 29/44) according to international criteria. Pathogenicity tests were performed on a susceptible local okra (A. esculentus) landrace in… More >

  • Open Access

    ARTICLE

    MicroRNA-145-3p suppresses the malignant behaviors of T-cell acute lymphoblastic leukemia Jurkat cells via inhibiting the NFkappaB signaling pathway

    Xin YANG*, Liqun LU, Li HUANG, Jing HE, Jie LV

    BIOCELL, Vol.44, No.1, pp. 101-110, 2020, DOI:10.32604/biocell.2020.08324

    Abstract T-cell acute lymphoblastic leukemia (T-ALL) is a hematological tumor caused by the malignant transformation of immature T-cell progenitor cells. Emerging studies have stated that microRNAs (miRNAs) may play key roles in T-ALL progression. This study aimed to investigate the roles of miR-145-3p in T-ALL cell proliferation, invasion, and apoptosis with the involvement of the nuclear factor-kappaB (NF-κB) signaling pathway. T-ALL Jurkat cells were harvested, and the expression of miR-145-3p and NF-κB-p65 was measured. Gain- and loss-of-functions of miR-145-3p and NF-κB-p65 were performed to identify their roles in the biological behaviors of Jurkat cells, including proliferation, apoptosis, and invasion. Consequently, the… More >

  • Open Access

    ARTICLE

    MicroRNA-708 inhibits the proliferation and chemoresistance of pancreatic cancer cells

    Wensong LIU1, Yunjie LU1, Dong ZHANG1, Longqing SHI1, Guangchen ZU1, Haijiao YAN2,*, Donglin SUN1,*

    BIOCELL, Vol.44, No.1, pp. 73-80, 2020, DOI:10.32604/biocell.2020.08613

    Abstract Pancreatic cancer is one of the most aggressive malignancies with poor prognosis and high mortality. Recent studies showed that microRNAs are dysregulated and involved in the initiation and progression of pancreatic cancer. In this study, we found that miR-708 was significantly downregulated in pancreatic cancer tissues and cell lines. Lentivirus-mediated overexpression of miR-708 could significantly inhibit the proliferation and invasion, while enhanced chemosensitivity to gemcitabine in both Panc-1 and SW1990 cells. Luciferase reporter assay showed that miR-708 bound the 3’-untranslated region of survivin and suppressed the expression of survivin in pancreatic cancer cells. In pancreatic cancer tissues, survivin protein was… More >

  • Open Access

    ARTICLE

    Analysis of the Relationship between Blast Resistance Genes and Disease Resistance of Rice Germplasm via Functional Molecular Markers

    Ning Xuan1, Hua Zhang1, Xu Liu1, Qiang Guo1, Jian Liu2, Fangyin Yao1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 45-55, 2020, DOI:10.32604/phyton.2020.08548

    Abstract Rice blast disease is one of the most devastating diseases of rice (Oryza sativa L.) caused by the fungus Magnaporthe oryzae (M. oryzae), and neck blast is the most destructive phase of this illness. The underlying molecular mechanisms of rice blast resistance are not well known. Thus, we collected 150 rice varieties from different ecotypes in China and assessed the rice blast resistances under the natural conditions that favoured disease development in Jining, Shandong Province, China in 2017. Results showed that 92 (61.3%) and 58 (38.7%) rice varieties were resistant and susceptible to M. oryzae, respectively. Among the 150 rice… More >

  • Open Access

    ARTICLE

    Aerodynamic Design of a Subsonic Evacuated Tube Train System

    Tian Li1, *, Xiaohan Zhang1, Yao Jiang2, Weihua Zhang1

    FDMP-Fluid Dynamics & Materials Processing, Vol.16, No.1, pp. 121-130, 2020, DOI:10.32604/fdmp.2020.07976

    Abstract The so-called Evacuated Tube Train (ETT) is currently being proposed as a high-speed transportation system potentially competitive with airplane transportation. Aerodynamic resistance is one of the most crucial factors for the successful design of an ETT. In the present work, a three-dimensional concept ETT model has been elaborated. The aerodynamic characteristics of the subsonic ETT have been numerically simulated under different conditions. The train’s running speed varies from 600 km/h up to 1200 km/h, and the blockage ratio is in the range between 0.1 and 0.3. As the blocking ratio and running speed increase, the resistance of the head car… More >

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