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  • Open Access

    ARTICLE

    SCAR molecular markers to identify disease resistance sources in ayocote beans (Phaseolus coccineus)

    Ruíz-Salazar R1, JS Muruaga-Martínez2, MLP Vargas-Vázquez2, G Alejandre-Iturbide4, G Castañón-Nájera3, S Hernández-Delgado1, N Almaraz-Abarca4, N Mayek-Pérez1,5

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 184-193, 2016, DOI:10.32604/phyton.2016.85.184

    Abstract In order to identify genes of resistance to four diseases that affect Phaseolus sp. [i.e, Common Bacterial Blight (CBB), Bean Common Mosaic Virus (BCMV), Bean Golden Mosaic Virus (BGMV) and angular leaf spot (ALS)], we amplified nine SCAR (Sequence Characterized Amplified Regions) markers: four linked to resistance to common blight, two for BCMV, two for BGMV and one for ALS. The genetic material included 16 ayocote bean (P. coccineus) populations collected from different locations at Veracruz and Puebla, Mexico. Populations from Veracruz showed greater number of SCARs for BGMV and ALS compared with populations from Puebla. More >

  • Open Access

    ARTICLE

    Enhancement of Drug Sensitivity by Knockdown of HIF-1α in Gastric Carcinoma Cells

    Qun Zhao, Bi-Bo Tan, Yong Li, Li-Qiao Fan, Pei-Gang Yang, Yuan Tian

    Oncology Research, Vol.23, No.3, pp. 129-136, 2015, DOI:10.3727/096504015X14500513118029

    Abstract In this study, the effects of hypoxia-inducible factor-1α (HIF-1α) on gastric carcinoma (GC) drug resistance through apoptosis-related genes are investigated. First, HIF-1α-specific siRNA was synthetized and transfected into drug-resistant GC cell line OCUM-2MD3/L-OHP. Then MTT assay was applied to test the inhibition rate of GC cells by 5-fluorouracil (5-FU) and oxaliplatin (L-OHP). After that, flow cytometry (FCM) was applied to measure apoptosis rate. qPCR and Western blot assay were employed to detect HIF-1α and apoptosis-related genes. Results showed that HIF-1α in OCUM-2MD3/L-OHP cells was higher than that in OCUM-2MD3 and gastric epithelial cells. After HIF-1α-siRNA More >

  • Open Access

    ARTICLE

    Factors influencing in the response of Schizolobium parahybum (Vell) Blake to Ceratocystis paradoxa and C. moniliformis

    Mora-Silva W1,3, FR Garcés-Fiallos1,2, C Suarez-Capello1,2, CE Belezaca-Pinargote1,3, P Cedeño-Loja1, E Vallejo3

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 120-127, 2015, DOI:10.32604/phyton.2015.84.120

    Abstract In the Ecuadorian coast one of the most destructive diseases of the pachaco is vascular wilt or stem rot caused by Ceratocystis complex, so the aim of this study was to determine the factors that affect the efficiency of the reaction of bark pachaco to this disease. This research was conducted under laboratory conditions, using trees pachaco S38, S41, S98, AE-1, AE-2 and AE-3, and pathogenic species Ceratocystis paradoxa and C. moniliformis. The method utilized was tissue stem bark,with bark sections with 4.5 cm2, and a suspension of 3x104 units infection and remained in a humid chamber for 96… More >

  • Open Access

    ARTICLE

    A COMPARATIVE STUDY ON THERMAL CONDUCTIVITY AND RHEOLOGY PROPERTIES OF ALUMINA AND MULTI-WALLED CARBON NANOTUBE NANOFLUIDS

    Zan Wua, Zhaozan Fengb, Bengt Sundéna,*, Lars Wadsöc

    Frontiers in Heat and Mass Transfer, Vol.5, pp. 1-10, 2014, DOI:10.5098/hmt.5.18

    Abstract Thermal conductivity and rheology behavior of two aqueous nanofluids, i.e., alumina and multi-walled carbon nanotube (MWCNT) nanofluids, were experimentally investigated and compared with previous analytical models. Information about the possible agglomeration size and interfacial thermal resistance in the nanofluids were obtained and partially validated. By incorporating the effects of interfacial thermal resistance, a revised model was found to accurately reproduce the experimental data based on the agglomeration size extracted from the rheology analysis. In addition, the thermal conductivity change of the alumina/water nanofluid with elapsed time was investigated. Thermal conductivity measurements were also conducted for More >

  • Open Access

    ARTICLE

    On the First-principles Density Functional Theory Calculation of Electromigration Resistance Ability for Sn-based Intermetallic Compounds

    Wen-Hwa Chen1,2, Ching-Feng Yu1, Hsien-Chie Cheng2,3

    CMES-Computer Modeling in Engineering & Sciences, Vol.100, No.2, pp. 119-131, 2014, DOI:10.3970/cmes.2014.100.119

    Abstract The aim of the study is to investigate the interactions between Sn adatoms in a solder bump and three typical Sn-based intermetallic compounds (IMCs) surface, i.e., Cu3Sn, Cu6Sn5, and Ni3Sn4, at the atomistic scale. The adsorption energy, average bond length, and bond population of the Sn/Cu3Sn, Sn/Cu6Sn5,and Sn/Ni3Sn4 systems are calculated through the first-principles density functional theory (DFT) calculation to investigate how the Sn adatoms influence the IMC surface. The calculated results show that the Sn atoms on the Cu3Sn (0 0 1) surface hold the largest adsorption energy, average bond length and bond population, implying that the Cu3Sn… More >

  • Open Access

    ARTICLE

    THERMAL CONDUCTIVITY OF BINARY MIXTURES OF GASES

    Etim S. Udoetok*

    Frontiers in Heat and Mass Transfer, Vol.4, No.2, pp. 1-5, 2013, DOI:10.5098/hmt.v4.2.3008

    Abstract A model for the coefficient of thermal conductivity of binary mixtures of gases has been derived. The theory presented is based on the assumption of random fluctuations between two possible extreme arrangements of a binary gas mixture. The results obtained from the new model compared favorably with published experimental results. The proposed new model provides a simple approach without sacrificing much accuracy compared to previous models. It is applicable to any binary mixture of gases which includes monoatomic gas mixture, polyatomic gas mixtures and mixtures involving rare gases. The new model can be very useful More >

  • Open Access

    ARTICLE

    Causes of the Water Resistance of Welded Joints of Paduk Wood (Pterocarpus soyauxii Taub.)

    T. Ganier, J. Hu, A. Pizzi*

    Journal of Renewable Materials, Vol.1, No.1, pp. 79-82, 2013, DOI:10.7569/JRM.2012.634101

    Abstract Linear vibration welding of extractive rich Paduk wood from central Africa containing a high proportion of a native mixture of water-insoluble extractives, or of low water solubility, has been shown to yield joints of much upgraded water resistance. This has been shown to be due to the protecting infl uence the extractives from the wood itself has on the welded interphase, due to their inherent water repellence. Joints of unusually high percentage wood failure but modest strength were obtained; Paduk wood brittleness apparently yielding weld line strengths always higher than that of the surrounding wood More >

  • Open Access

    ARTICLE

    Advances and perspectives on the gene mapping of root rot resistance in common beans

    Méndez-Aguilar R1, MH Reyes-Valdés2, N Mayek-Pérez3

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 215-226, 2013, DOI:10.32604/phyton.2013.82.215

    Abstract Common bean was originated in México, and it is the most important protein source for millions of Mexicans, mainly those with low economic incomes. Grain yield of this crop is mainly affected by several fungal, bacterial and viral diseases. Among them, there are root rot diseases caused by the genera Macrophomina and Fusarium sp. Currently, some molecular biology tools such as DNA molecular markers are being used successfully to develop genetic maps where QTLs and major molecular markers are located, which are linked to genes that confer resistance to these fungi. The importance of the development… More >

  • Open Access

    ARTICLE

    Determination of insecticide resistance in four populations of potato psillid Bactericera cockerelli (Sulc.) (Hemiptera: Triozidae)

    Cerna E, Y Ochoa, LA Aguirre, M Flores, J Landeros

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 63-68, 2013, DOI:10.32604/phyton.2013.82.063

    Abstract Southeastern potato growing regions from Coahuila and Nuevo León contribute with 15% to domestic production. Such production has been impacted by several factors, in particular Bactericera (=Paratrioza) cockerelli Sulc potato psyllid, one of the most important pests affecting this crop. Its control is based on Insecticide applications, leading to unsatisfactory results and high selection pressure. Five insecticides were evaluated in four field populations of B. cockerelli from the growing region, using a susceptible laboratory line for comparison. The field populations were collected from greenhouse and commercial orchards located at Huachichil, Saltillo, Raíces and San Rafael localities. All… More >

  • Open Access

    ARTICLE

    High passage numbers induce resistance to apoptosis in C2C12 muscle cells

    LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO*

    BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001

    Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of More >

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