Youwei Zhang^*, Bi Chen^†, Yongsheng Wang^‡, Qi Zhao^‡, Weijun Wu^§, Peiying Zhang^*, Liyun Miao^‡, Sanyuan Sun^*

Oncology Research, Vol.27, No.7, pp. 751-761, 2019, DOI:10.3727/096504018X15372657298381

Abstract Acquired resistance remains a key challenge in epidermal growth factor receptor (EGFR)–tyrosine kinase inhibitors (TKIs) therapy in lung adenocarcinoma (LUAD). Recent studies have shown that Notch signaling is associated with drug resistance. However, its role and possible mechanisms in EGFR-TKI resistance are not yet clear. In our study, we found that among four members of NOTCH1–4, only NOTCH3 was upregulated in LUAD tissues and TKI-resistant cell line (HCC827GR6). Knockdown of NOTCH3 by siRNA significantly inhibited proliferative ability, and decreased colony and sphere formation in HCC827GR6 cells. Then miR-150 was identified as a posttranscriptional regulator of… More >

Wanjun Yu^*, Weidong Peng^*, Hanyun Sha^†, Jipeng Li^‡

Oncology Research, Vol.27, No.5, pp. 623-628, 2019, DOI:10.3727/096504018X15420734828058

Abstract Circular RNAs (circRNAs) represent a new class of noncoding RNAs that is involved in the development of cancer. However, little is known about their role in chemoresistance. In the present study, we found that hsa_circ_0003998 expression levels in lung adenocarcinoma (LAD) tissues and docetaxel-resistant cell lines (A549/DTX and H1299/DTX) were upregulated. Knockdown of hsa_circ_0003998 decreased chemoresistance, inhibited proliferation, and enhanced apoptosis in docetaxel-resistant LAD cells. Moreover, by using bioinformatics and luciferase reporter assays, we found that miR-326 was a direct target of hsa_circ_0003998. Functional analysis revealed that miR-326 mediated the effect of hsa_circ_0003998 on chemosensitivity. More >

Ting Yu^*1, Lei Li^†1, Wenyan Liu^*, Bailiu Ya^*, Hongju Cheng^*, Qing Xin^*

Oncology Research, Vol.27, No.5, pp. 525-532, 2019, DOI:10.3727/096504018X15179668157803

Abstract Hypoxia-induced chemoresistance is a major obstacle in the development of effective cancer therapy. In our study, the reversal abilities of NADPH oxidase 4 (NOX4) silence on hypoxia resistance and the potential mechanism were investigated. Our data showed that the expression of NOX4 was upregulated in human neuroblastoma cells SH-SY5Y under hypoxia condition time dependently. Knockdown of NOX4 expression by siRNA inhibited glycolysis induced by hypoxia through decreasing the expression of glycolysis-related proteins (HIF-1 , LDHA, and PDK1), decreasing glucose uptake, lactate production, and ROS production, while increasing mitochondria membrane potential. Moreover, NOX4 silence inhibited cell… More >

T. Pudlarz, N. Naoun, G. Beinse, D. Grazziotin-Soares, J.-P. Lot

Oncologie, Vol.21, No.1, pp. 53-68, 2019, DOI:10.3166/onco-2019-0036

Abstract Dans ce numéro spécial de la revue Oncologie, les principaux points discutés au congrès de l’Association amé- ricaine pour la recherche sur le cancer (AACR) sont rapportés. L’objectif ici est de présenter de manière concise des exposés qui méritent une attention toute particulière. Le programme de la réunion de l’AACR de cette année, qui a eu lieu à Atlanta, a couvert les dernières découvertes de tout le spectre de la recherche sur le cancer — des sciences de la population à la prévention ; biologie du cancer, études translationnelles et cliniques ; à la survie… More >

V. Massard, A. Harlé, L. Uwer, J.-L. Merlin

Oncologie, Vol.21, No.1, pp. 29-32, 2019, DOI:10.3166/onco-2019-0027

Abstract L’hormonorésistance acquise constitue l’un des défis majeurs dans le traitement du cancer du sein avancé exprimant le récepteur aux estrogènes (RE) et sans surexpression de HER2. Les mutations activatrices du gène ESR1 affectant le domaine de liaison du ligand ont récemment été identifiées comme l’un des principaux mécanismes de résistance aux inhibiteurs de l’aromatase (IA). Ces mutations peuvent être recherchées sur des prélèvements histologiques ou sur ADN tumoral circulant, par PCR ou séquen- çage de nouvelle génération (NGS). Elles induisent une activation constitutionnelle du RE conduisant à une résistance acquise aux IA ; le tamoxifène, le More >

Ranjit Philip¹, Jason Nathaniel Johnson^1,2, Ronak Naik¹, Dai Kimura^1,3, Umar Boston¹, Sandeep Chilakala¹, Benjamin Hendrickson¹, Benjamin Rush Waller¹, Shyam Sathanandam¹

Congenital Heart Disease, Vol.14, No.1, pp. 37-41, 2019, DOI:10.1111/chd.12702

Abstract The hemodynamic effects of a patent ductus arteriosus (PDA) are well known including systemic hypoperfusion and volume overload on the left ventricle. This article aims to provide a review of the long-standing effect of a hemodynamically significant PDA on the pulmonary vasculature and the role of cardiac catheterization in preterm infants with a PDA and pulmonary hypertension. More >

Yuan LI¹, Yonghyun LEE², Yielhea SEO³, Youjin HWANG^{1, 2, *}

BIOCELL, Vol.43, No.4, pp. 263-269, 2019, DOI:10.32604/biocell.2019.07664

Abstract The aim of this study was to determine the relationship between phenotypic antimicrobial susceptibility patterns and extended-spectrum, carbapenem-resistance genes. A total of 109 clinical Staphilococcus aureus strains were subjected to 19 antimicrobial susceptibility tests. Resistance to methicillin (mecA), penicillin (blaTEM), and tetracycline (tetM) was detected. We compared the presence of the blaTEM genes with extended-spectrum, carbapenem-related genes and identified the types of SCCmec genes. Of 109 clinical S. aureus strains, 62 (56.88%) had methicillin resistance and 60 strains carried mecA. The prevalence of blaTEM and tetM genes was 81.65% and 37.61%, respectively. The most predominant SCCmec type More >

Dairong LI¹, Xianlu ZHUO^1,2, Lumi HUANG¹, Xiaohui JI¹, Donglin WANG¹

BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460

Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were performed to assess cell viability, apoptosis, and More >

Lídia K. Lazzari^*, Vitória B. Zampieri, Ademir J. Zattera, Camila Baldasso

Journal of Renewable Materials, Vol.7, No.3, pp. 227-234, 2019, DOI:10.32604/jrm.2019.00024

Abstract This work aimed at analyzing the petroleum adsorption capacity and the compressive strength of cellulose cryogels produced with the addition of 4 and 8% (w/w) sodium hydroxide (NaOH). Cryogels were produced from a 1.5% (m/m) suspension of cellulose microfibers, which originated from Pinus elliotti, in distilled water. The addition of NaOH increased the compression strength of cryogel by 30% when compared to cryogel without the reagent. Due to the 50% decrease in volume, the cryogel FLNB-8h (8% NaOH) has its petroleum adsorption capacity decreased in average by 58%, whereas the FLNB-4 (4% NaOH) cryogel had More >

Eunsoo Choi^1,*, Yunkyu Ha¹, Duhyeon Kim¹, Hoan D. Nguyen¹, Tae Yeong Kim¹, Seong Jun Park¹, Yeon-seong Lee¹, Cheolki Min²

The International Conference on Computational & Experimental Engineering and Sciences, Vol.21, No.1, pp. 13-13, 2019, DOI:10.32604/icces.2019.05356

Abstract This study was to provide a new aspect of application of shape memory alloys (SMAs) in civil engineering. The high-density polyethylene (HDPE) have been used for construction of pipelines for several years. The main problem of using HDPE for pipes is the joint of two adjacent pipes. In case of having a perfect joint, the HDPE pipeline can bare the internal pressure up to 90 MPa. However, in practice, it can rarely achieve to a perfect pipe joint due to the complicated process, so, it prone to fracture in low internal pressure. To overcome this,… More >