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  • Open Access

    ARTICLE

    Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid, Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

    Longhua Zhou1,2,#, Ting He1,2,#, Jing Li1,2,3, Guimei Guo1,2, Yingbo Li1,2, Hongwei Xu1,2, Runhong Gao1,2, Linli Huang1,2, Yifei Wang1,2, Ruiju Lu1,2, Zhiwei Chen1,2,*, Chenghong Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912

    Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

  • Open Access

    ARTICLE

    Constructing Collective Signature Schemes Using Problem of Finding Roots Modulo

    Tuan Nguyen Kim1,*, Duy Ho Ngoc2, Nikolay A. Moldovyan3

    CMC-Computers, Materials & Continua, Vol.72, No.1, pp. 1105-1122, 2022, DOI:10.32604/cmc.2022.025653

    Abstract Digital signature schemes are often built based on the difficulty of the discrete logarithm problems, of the problem of factor analysis, of the problem of finding the roots modulo of large primes or a combination of the difficult problems mentioned above. In this paper, we use the new difficult problem, which is to find the root in the finite ground field to build representative collective signature schemes, but the chosen modulo p has a special structure distinct , where is an even number and are prime numbers of equal magnitude, about . The characteristics of the proposed scheme are: i)… More >

  • Open Access

    ARTICLE

    POM analysis and computational interactions of 8-hydroxydiospyrin inside active site of protein tyrosine phosphatase 1B

    SAUD BAWAZER1, ASGHAR KHAN2, ABDUR RAUF3,*, TAIBI BEN HADDA4, YAHYA S. AL-AWTHAN5,6, OMAR BAHATTAB5, UMER RASHID7, INAMULLAH KHAN8, MUHAMMAD ASIF NAWAZ9, MD SAHAB UDDIN10,11, OLATUNDE AHMED12, MOHAMMAD ALI SHARIATI13

    BIOCELL, Vol.45, No.3, pp. 751-759, 2021, DOI:10.32604/biocell.2021.014004

    Abstract Protein tyrosine phosphatase 1B (PTP1B) inhibition is considered as a potential therapeutic for the treatment of cancer, type 2 diabetes, and obesity. In our present work, we investigated the anti-diabetic potential of 8-hydroxydiospyrin (8-HDN) from D. lotus against the PTP1B enzyme. It showed significant inhibitory activity of PTP1B with an IC50 value of 18.37 ± 0.02 μM. A detailed molecular docking study was carried out to analyze the binding orientation, binding energy, and mechanism of inhibition. A comparative investigation of 8-HDN in the catalytic, as well as the allosteric site of PTP1B, was performed. Binding energy data showed that compound… More >

  • Open Access

    ARTICLE

    Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds

    Anna Stepanova1,*, Maria Malunova1, Svetlana Salamaikina1, Renat Selimov2, Aleksandra Solov’eva1

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223

    Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses… More >

  • Open Access

    ARTICLE

    Effects of Exogenous Manganese (Mn) on Mineral Elements, Polyamines and Antioxidants in Apple Rootstock Malus robusta Rehd.

    Dazhuang Qi1,2,4,#, Meixia Liang1,2,4,#, Fudong Jiang3, Jianzhao Li1,2,4, Xuqiang Qiao1,2,4,*, Hongxia Zhang1,2,4,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 943-955, 2020, DOI:10.32604/phyton.2020.013801

    Abstract Manganese (Mn) is one of the essential microelements in all organisms. However, high level of Mn is deleterious to plants. In this study, the effects of exogenous manganese application on mineral element, polyamine (PA) and antioxidant accumulation, as well as polyamine metabolic and antioxidant enzyme activities, were investigated in Malus robusta Rehd., a widely grown apple rootstock. High level of Mn treatments decreased endogenous Mg, Na, K and Ca contents, but increased Zn content, in a Mn-concentration-dependent manner. Polyamine metabolic assays revealed that, except the content of perchloric acid insoluble bound (PIS-bound) spermine, which increased significantly, the contents of putrescine… More >

  • Open Access

    ARTICLE

    Computer Methodologies for the Comparison of Some Efficient Derivative Free Simultaneous Iterative Methods for Finding Roots of Non-Linear Equations

    Yuming Chu1, Naila Rafiq2, Mudassir Shams3,*, Saima Akram4, Nazir Ahmad Mir3, Humaira Kalsoom5

    CMC-Computers, Materials & Continua, Vol.66, No.1, pp. 275-290, 2021, DOI:10.32604/cmc.2020.011907

    Abstract In this article, we construct the most powerful family of simultaneous iterative method with global convergence behavior among all the existing methods in literature for finding all roots of non-linear equations. Convergence analysis proved that the order of convergence of the family of derivative free simultaneous iterative method is nine. Our main aim is to check out the most regularly used simultaneous iterative methods for finding all roots of non-linear equations by studying their dynamical planes, numerical experiments and CPU time-methodology. Dynamical planes of iterative methods are drawn by using MATLAB for the comparison of global convergence properties of simultaneous… More >

  • Open Access

    ARTICLE

    Optimization of Roots and Copper Slag to Reinforce Soft Soil Using Response Surface Method

    Dingbang Zhang1, Yi Zhang2,*, Zhiguo Cao3, Tao Cheng1

    Journal of Renewable Materials, Vol.8, No.11, pp. 1391-1409, 2020, DOI:10.32604/jrm.2020.012695

    Abstract In this paper, roots and copper slag were used to overcome the weak- ness and reinforce the mechanical property of soft soil. The experiments were designed by the Response Surface Method (RSM), the content optimizing of the root permeated copper slag mixed soil for achieving appropriate values of shear strength and the final results evaluating were also conducted by RSM. Four independent variables including moisture content (12–21% by dry weight of the mixture), slag content (10–20% by dry weight of the mixture), roots content (0–1.1% by dry weight of the mixture), and aspect ratio of roots L/d (5–40) were studied… More >

  • Open Access

    ARTICLE

    Chromium Differentially Affects Hydrogen Peroxide Distribution in Primary and Adventitious Roots of Arabidopsis thaliana L.

    Aarón Giovanni Munguía-Rodríguez1,2, José López-Bucio1, Gerardo Rangel Sánchez1,2, León Francisco Ruiz-Herrera1, Yazmín Carreón-Abud2, Miguel Martínez-Trujillo2,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 35-43, 2020, DOI:10.32604/phyton.2020.07835

    Abstract The post-embryonic growth of the Arabidopsis thaliana root system can be modified by different types of stress, such as sublethal concentrations of metals, which may induce the production of reactive oxygen species (ROS). In this study, the effects of different concentrations of potassium chromate (KCrO4) on the distribution and relative quantity of hydrogen peroxide (H2O2) were determined in primary and adventitious roots in A. thaliana HyPer line seedlings. This line has a biosensor that specifically reports H2O2 levels within tissues as fluorescence. Primary root growth was inhibited at 100 μM Cr (VI); in contrast, adventitious root formation was induced over… More >

  • Open Access

    ARTICLE

    Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina

    LUIS A. CARO1, NATALIA SANTECCHIA1, PABLO A. MARINANGELI1, NÉSTOR R. CURVETTO1,2, LUIS F. HERNÁNDEZ1,3

    BIOCELL, Vol.27, No.3, pp. 311-318, 2003, DOI:10.32604/biocell.2003.27.311

    Abstract The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with… More >

  • Open Access

    ARTICLE

    Salinity induced anatomical and morphological changes in Chloris gayana Kunth roots

    GABRIEL CÉCCOLI*1, JULIO C. RAMOS1, LEANDRO I. ORTEGA2 , JUAN M. ACOSTA1, MARIEL G. PERRETA1

    BIOCELL, Vol.35, No.1, pp. 9-17, 2011, DOI:10.32604/biocell.2011.35.009

    Abstract Chloris gayana Kunth is a grass species valuable as forage which was introduced into Argentina to be used as pasture in saline soils of subtropical and warm-temperate zones, given its good adaptability to drought, salinity and mild freezing. However, its tolerance varies according to the cultivar. In tetraploid cultivars, important reductions in yield have been observed. Here, a study of the variations produced on the root and stem system by salinity at different NaCl concentrations (0, 150 y 250 mM) was performed in the Boma cultivar, with the aim of determining the anatomical and morphological alterations produced by the salt… More >

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