Baohong Yang^*1, Aiying Qin^†1, Kongyuan Zhang^‡, Haipeng Ren^*, Shuzhen Liu^*, Xiaolei Liu^§, Xiangpo Pan^¶, Guohua Yu^*

Oncology Research, Vol.25, No.9, pp. 1601-1606, 2017, DOI:10.3727/096504017X14928634401178

Abstract Epithelial growth factor receptor (EGFR) mutations are present in 10%–26% of non-small cell lung cancer (NSCLC) tumors and are associated with the response to tyrosine kinase inhibitors (TKIs). This study aimed to detect and quantify the presence of circulating tumor cells (CTCs) in EGFR-mutant NSCLC patients and investigate their possible role in providing prognostic information. Enrolled patients received erlotinib (150 mg) or gefitinib (250 mg) orally once daily as the first-line treatment. Serial blood samples were taken at baseline (CTC-d0) and on day 28 (CTC-d28) following the initiation of erlotinib/gefitinib for detection of CTCs using… More >

Jianping Zhou^*, Fan Wang^†, Bingli Liu^‡, Lin Yang^*, Xueying Wang^*, Yu Liu^*

Oncology Research, Vol.25, No.6, pp. 931-937, 2017, DOI:10.3727/096504016X14772424117423

Abstract Pediatric glioma is a devastating brain tumor. Serine threonine tyrosine kinase 1 (STYK1) is a member of the protein tyrosine kinase family and plays a significant role in the formation of several malignant tumors. However, the expression pattern and role of STYK1 in glioma are not yet clear. The aim of this study was to investigate the role and molecular mechanism of STYK1 in glioma. The results showed that STYK1 was highly expressed in glioma cell lines. We also found that knockdown of STYK1 inhibited cell proliferation, migration, and invasion in vitro as well as More >

Chun-ming Yang^*, Shan Ji^†, Yan Li^‡, Li-ye Fu^‡, Tao Jiang^‡, Fan-dong Meng^‡

Oncology Research, Vol.25, No.2, pp. 195-205, 2017, DOI:10.3727/096504016X14732772150424

Abstract Renal cell carcinoma (RCC) represents one of the most resistant tumors to radiation and chemotherapy. Current therapies for RCC patients are inefficient due to the lack of diagnostic and therapeutic markers. The expression of novel tumor-associated kinases has the potential to dramatically shape tumor cell behavior. Identifying tumor-associated kinases can lend insight into patterns of tumor growth and characteristics. In the present study, we investigated the receptor tyrosine kinase-like orphan receptor 2 (Ror2), a new tumor-associated kinase, in RCC primary tumors and cell lines. Knockdown of Ror2 expression in RCC cells with specific shRNA significantly More >

Nishant Patel¹, Jason Woo¹, Michael A. Liss¹, Kerrin L. Palazzi¹, J. Michael Randall², Reza Mehrazin³, Ramzi Jabaji¹, Hossein S. Mirheydar¹, Kyle Gillis¹, Hak J. Lee¹, Anthony L. Patterson³, Christopher J. Kane¹, Frederick Millard², Ithaar H. Derweesh¹

Canadian Journal of Urology, Vol.23, No.2, pp. 8227-8233, 2016

Abstract Introduction: To compare surgical complications and tyrosine kinase inhibitor (TKI)-toxicities in patients who underwent primary cytoreductive nephrectomy (CN) followed by adjuvant TKI therapy versus those who underwent neoadjuvant TKI therapy prior to planned CN for metastatic renal cell carcinoma (mRCC).
Materials and methods: Two-center retrospective analysis. Sixty-one mRCC patients underwent TKI therapy with sunitinib between July 2007 to January 2014. Patients were divided into three groups: primary CN followed by adjuvant TKI (n = 27, Group 1), neoadjuvant TKI prior to CN (n = 21, Group 2), and primary TKI alone (no surgery, n = 13, Group… More >

MANOOCHEHR MESSRIPOUR¹, AZADEH MESRIPOUR²

BIOCELL, Vol.37, No.2, pp. 17-21, 2013, DOI:10.32604/biocell.2013.37.017

Abstract Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosynthesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age, this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Synaptosomes were incubated in the presence of added pargiline (monoamineoxidase inhibitor), dopamine or serotonin synthesized during 25 min was measured by HPLC, employing electrochemical… More >

Daniel Canter, Alexander Kutikov, Konstantin Golovine, Petr Makhov, Jay Simhan, Robert G. Uzzo, Vladimir M. Kolenko

Canadian Journal of Urology, Vol.18, No.4, pp. 5819-5825, 2011

Abstract Objectives: We examined the in vitro cellular effects of the multi-targeted receptor tyrosine kinase inhibitors (TKIs) sunitinib and pazopanib on a series of human renal cell carcinoma (RCC) cell lines.
Methods: The human RCC cell lines 769-P, 786-O, HRC-24, HRC-31, HRC-45, HRC-78, SK-26B, and SK-45 were treated with varying concentrations of sunitinib and pazopanib. Cellular proliferation and cell death were assessed using the CellTiter-Blue Cell Viability Assay and the TUNEL assay, respectively. Effective doses (ED) for inhibition of cellular proliferation or induction of apoptosis were calculated for both sunitinib and pazopanib in each RCC cell line.
Results: Both… More >

Gokhan Koc¹, Xu Wang², Yi Luo³

Canadian Journal of Urology, Vol.18, No.6, pp. 5991-5997, 2011

Abstract Introduction: Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults, responsible for approximately 90% of all kidney cancers. Prior to 2005, treatment options for patients with locally advanced and metastatic disease were limited. After the approval of sorafenib by the US Food and Drug Administration (FDA), other tyrosine kinase inhibitors (TKI) have been successively used for treating patients with advanced RCC. Pazopanib is the newest, orally bioavailable, and multi-targeted TKI, and is considered a first-line treatment option for certain patients. This review summarizes updated clinical studies, mechanism of action, and pharmacokinetics… More >

Scott Manatt, Adam Metwalli, Daniel J. Culkin

Canadian Journal of Urology, Vol.13, No.4, pp. 3199-3200, 2006

Abstract Sclerosing mesenteritis is part of a spectrum of benign, inflammatory diseases of the abdominal mesentery. It has been called retractile mesenteritis, and other forms include mesenteric panniculitis and mesenteric lipodystrophy. The disease rarely involves the retroperitoneum. We present a case of sclerosing mesenteritis that progressed to ureteral involvement. More >

ROBERTO YUNES, PEDRO FERNÁNDEZ, GUSTAVO F. DONCEL, ANÍBAL A. ACOSTA

BIOCELL, Vol.29, No.3, pp. 287-293, 2005, DOI:10.32604/biocell.2005.29.287

Abstract Our objective was to determine the effect of phosphodiesterase (PDE) inhibition on: 1) tyrosine phosphorylation of human spermatozoa at the tail level; and 2) sperm motion parameters and hyperactivated motility. The study was conducted with normozoospermic and asthenozoospermic samples incubated under in vitro capacitating conditions. The main outcome measures were computer-assisted sperm motion analysis and fluorescent immunodetection of phosphotyrosine-containing proteins. Pentoxifylline (PTX) was used as PDE inhibitor because of its wide use in the clinic. PTX-treatment significantly increased sperm velocity, hyperactivated motility and tyrosine-phosphorylation, both in normo and asthenozoospermic samples. Tyrosine-phosphorylation of tail proteins was highly More >

M. I. ROSATTI, M. T. BECONI, M. CÓRDOBA

BIOCELL, Vol.28, No.3, pp. 311-316, 2004, DOI:10.32604/biocell.2004.28.311

Abstract Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level More >