Lovey¹ RJ, P Perissé², C V ieyra³, JC Coraglio³

Phyton-International Journal of Experimental Botany, Vol.79, pp. 5-10, 2010, DOI:10.32604/phyton.2010.79.005

Abstract Cologania broussonetii (Balb.) DC. (Fabaceae) grows from the Bolivian eastern Andes to the north west of Argentina, Cordoba hills and San Luis Province. It is recognized as a native forage. The objectives of this study were to characterize the seed, the germination and the seedling of C. broussonetii. Seed structure was characterized through free hand sections and analyzed with optical and stereoscopic microscopy. The germination assay treatments were: (1) intact non-scarfied seeds at 25 °C constant, (2) scarified seeds at 25 °C constant, (3) intact non-scarified seeds at 20-30 °C alternating, and (4) scarified seeds at 20-30… More >

Diniz SPSS, RC Oliveira

Phyton-International Journal of Experimental Botany, Vol.78, pp. 155-160, 2009, DOI:10.32604/phyton.2009.78.155

Abstract The effects of fusaric acid, a mycotoxin produced by Fusarium sp, were investigated in relation to its action on corn seedlings. The mycotoxin inhibited the development of corn seedlings at 0.2 mM, causing root length reduction. Anatomically, alterations were only visible from 0.5 mM fusaric acid, directly influencing the cell differentiation process. Precocious differentiation reduces the elongation region. This explains (1) that root shortening is morphologically visible, and (2) the differentiation of a great number of lateral roots nearby the apex, which can be seen in longitudinal cuts. More >

Menéndez-Yuffá¹ A, L Ríos-Bolívar²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a More >

Galatro Andrea, Ivan Rousseau, Susana Puntarulo

Phyton-International Journal of Experimental Botany, Vol.75, pp. 7-20, 2006, DOI:10.32604/phyton.2006.75.007

Abstract Electron Paramagnetic Resonance (EPR) has been developed as a versatile field that uses different techniques sharing the common feature of resonant micro-wave radiation absorption by paramagnetic substances. Certain free radicals, such as ascorbyl radical (A•), show an stable spectrum, and can be directly detected by EPR at room temperature. Studies included in this work clearly show that this highly sensitive methodology can be successfully applied to biological systems. Exposure of plants to UV-B radiation was studied as a factor of oxidative stress in plants, and the effect on A^• steady state concentration was analyzed. A kinetic More >