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  • Open Access

    ARTICLE

    Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes

    PAOLA TALIA1 , EDUARDO J. GREIZERSTEIN1 , H. ESTEBAN HOPP1,2 , NORMA PANIEGO1 , LIDIA POGGIO2 AND RUTH A. HEINZ1,2*

    BIOCELL, Vol.35, No.1, pp. 19-28, 2011, DOI:10.32604/biocell.2011.35.019

    Abstract Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG More >

  • Open Access

    ABSTRACT

    Segmentation methods for human motion analysis from image sequences

    Maria João M. Vasconcelos1, João Manuel R. S. Tavares1

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.10, No.1, pp. 3-4, 2009, DOI:10.3970/icces.2009.010.003

    Abstract In the last years, researchers from the Computational Vision working field have been developing new methods to perform image segmentation for human motion analysis. The development of computational techniques suitable to automatically identify the structures involved is necessary to obtain more representative and robust features to be further used in the analysis of human motion from image sequences.
    The first step of human motion analysis from image sequences is strongly related with image segmentation. In fact, the first goal of any system designed for this aim is the identification of the structures’ features to be analysed… More >

  • Open Access

    ARTICLE

    Tracking Features in Image Sequences with Kalman Filtering, Global Optimization, Mahalanobis Distance and a Management Model

    Raquel R. Pinho1, João Manuel R. S. Tavares1

    CMES-Computer Modeling in Engineering & Sciences, Vol.46, No.1, pp. 51-76, 2009, DOI:10.3970/cmes.2009.046.051

    Abstract This work addresses the problem of tracking feature points along image sequences. In order to analyze the undergoing movement, an approach based on the Kalman filtering technique has been used, which basically carries out the estimation and correction of the features' movement in every image frame. So as to integrate the measurements obtained from each image into the Kalman filter, a data optimization process has been adopted to achieve the best global correspondence set. The proposed criterion minimizes the cost of global matching, which is based on the Mahalanobis distance. A management model is employed More >

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