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  • Open Access

    ARTICLE

    De novo Transcriptome Analysis in Leymus mollis to Unveil Genes Involved in Salt Stress Response

    Wenting Wu1 , Yajing Zhang1, Yu Gao1, Kai Zhang2,3, Luying Zhu1,3,*, Hongxia Zhang2,3

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1629-1642, 2022, DOI:10.32604/phyton.2022.020515 - 14 April 2022

    Abstract Leymus mollis, a wild relative of wheat, is very tolerant to salt stress, and has been considered as a valuable genetic resource for wheat breeding. However, the genetic basis for salt tolerance of this species is still largely unknown. In this study, de novo sequencing, assembly and analysis of L. mollis transcriptome in response to salt stress was performed. A total of 110,323 and 112,846 unigenes were generated for the NaCl-free (CK) and 180 mM NaCl-treated (CT) library, respectively. For the two libraries, 73,414 unigenes were successfully annotated in five common protein databases, and 7521 differentially expressed genes… More >

  • Open Access

    ARTICLE

    Villin Family Members Associated with Multiple Stress Responses in Cotton

    Fenni Lv1,2,#, Sen Wang3,#, Ruiping Tian1, Peng Wang2, Kang Liu1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1645-1660, 2021, DOI:10.32604/phyton.2021.016947 - 28 June 2021

    Abstract Villin (VLN) is considered to be one of the most important actin-binding proteins, participates in modulating the actin cytoskeleton dynamics, plays essential role in plant development and resisting adverse environments. However, systematic studies of the VLN gene family have not been reported in cotton (Gossypium). In this study, 14 GhVLNs were identified in G. hirsutum. These GhVLN genes were distributed in 6 A-subgenome chromosomes and 6 D-subgenome chromosomes of the allotetraploid upland cotton and classified into three phylogenetical groups based on the classification model of AtVLNs. In addition, the 14 GhVLN genes have highly conserved gene structure and motif… More >

  • Open Access

    ARTICLE

    Genome-Wide GRAS Gene Family Analysis Reveals the Classification, Expression Profiles in Melon (Cucumis melo L.)

    Yanfei Bi1, Bin Wei1, Ying Meng2, Zhongzhao Li2, Zhenghui Tang1, Feng Yin3, Chuntao Qian2,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1161-1175, 2021, DOI:10.32604/phyton.2021.014396 - 27 April 2021

    Abstract Melon (Cucumis melo), belonging to the Cucurbitaceae family, is a globally important economic crop. GRAS (GAI, RGA, SCR) genes, which are a type of transcription factor, play a critical role in plant growth and development, including processes such as radial root patterning, light signalling, abiotic/biotic stress, axillary shoot meristem formation, and phytohormone (gibberellin) signal transduction. In this study, the GRAS family in melon was analysed comprehensively with respect to chromosomal location, motif prediction, gene structure, and expression pattern. A total of 37 GRAS genes were first identified in melon, after which a phylogenetic tree was built… More >

  • Open Access

    ARTICLE

    Fatigue Performance Analysis and Evaluation for Steel Box Girder Based on Structural Health Monitoring System

    Meiling Zhuang1,2,3, Changqing Miao1,2,*, Rongfeng Chen1,2

    Structural Durability & Health Monitoring, Vol.14, No.1, pp. 51-79, 2020, DOI:10.32604/sdhm.2020.07663 - 01 March 2020

    Abstract Taizhou Yangtze River Bridge as a long-span suspension bridge, the finite element model (FEM) of it is established using the ANSYS Software. The beam4 element is used to simulate the main beam to establish the “spine beam” model of the Taizhou Yangtze River Bridge. The calculated low-order vibration mode frequency of the FEM is in good agreement with the completion test results. The model can simulate the overall dynamic response of the bridge. Based on the vehicle load survey, the Monte Carlo method is applied to simulate the traffic load flow. Then the overall dynamic… More >

  • Open Access

    ARTICLE

    Water stress response of Tricepiro and its progenitors Triticale and Trigopiro

    Ruiz MA1,3, A Golberg1,2, ML Molas1

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 263-274, 2014, DOI:10.32604/phyton.2014.83.263

    Abstract In semiarid regions rainfall is often scarce and erratic in space and time, making valuable the exploration of news genotypes with superior behavior in dry land systems. Tricepiro (X Triticosecale Wittmack x X Agrotriticum Ciferri & Giacom) is a synthetic cereal with long cycle and high biomass production in semiarid environments. However its performance under controlled water stress has not yet been evaluated. The aim of this work was to investigate the effect of water stress on tricepiro genotypes in comparison with their progenitors: triticale and trigopiro. Morphophysiological traits related to forage yield were evaluated in the… More >

  • Open Access

    ARTICLE

    Cloning and expression analysis of rubredoxin from cold-treated banana leaves

    Feng1 RJ, LF Lu2, KH Yuan3, P Cheng3, LL Zhang3, JF Qi3, Y Ren4, XL Xu5, XB Zhang3, LY Zhou3, YD Zhang3

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 163-168, 2010, DOI:10.32604/phyton.2010.79.163

    Abstract A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 More >

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