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  • Metabolomics Analysis of Metabolites in Forsythia suspense Fruit Using UPLC/ESI-Q TRAP-MS/MS
  • Abstract Forsythiae Fructus, the fruit of Forsythia suspense is a traditional Chinese hebal medicine that has the antiviral and antioxidant effects in China. Modern analytical chemistry studies showed that the extracts of Forsythiae Fructus contain many bioactive components, such as flavonoids, lignans, phenolic acids, and terpenoids, which can be used to anti-inflammatory and treat toxicity, tonsillitis, ulcers, pharyngitis and acute nephritis. In order to study the types and quantities of metabolites in Forsythiae Fructus, we isolated, identified and analysed metabolites between two varieties of Forsythiae Fructus using UPLC/ESI-Q TRAP-MS/MS. The results showed that a total of 407 metabolites were identified in…
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  • Extracting Lotus Fields Using the Spectral Characteristics of GF-1 Satellite Data
  • Abstract The lotus (Nelumbo nucifera Gaertn.) is an aquatic plant that grows in shallow water and has long been cultivated in South China. It can improve the incomes of farmers and plays an important role in alleviating poverty in rural China. However, a modern method is required to accurately estimate the area of lotus fields. Lotus has spectral characteristics similar to those of rice, grassland, and shrubs. The features surrounding areas where it is grown are complex, small, and fragmented. Few studies have examined the remote sensing extraction of lotus fields, and automatic extraction and mapping are still challenging methods. Here,…
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  • Tea Plantation Frost Damage Early Warning Using a Two-Fold Method for Temperature Prediction
  • Abstract As the source and main producing area of tea in the world, China has formed unique tea culture, and achieved remarkable economic benefits. However, frequent meteorological disasters, particularly low temperature frost damage in late spring has seriously threatened the growth status of tea trees and caused quality and yield reduction of tea industry. Thus, timely and accurate early warning of frost damage occurrence in specific tea garden is very important for tea plantation management and economic values. Aiming at the problems existing in current meteorological disaster forecasting methods, such as difficulty in obtaining massive meteorological data, large amount of calculation…
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  • Phytochemical Analysis, Estimation of Quercetin, and in Vitro Anti-Diabetic Potential of Stevia Leaves Samples Procured from Two Geographical Origins
  • Abstract The current study used RP-HPLC to compare phytochemicals and estimate the bioactive constituents found in Stevia rebaudiana Bert. (SRB) leaves collected from two different geographical sources. SRB leaves were collected from Bangalore, Karnataka, India, and Reduit, Mauritius. Extracts were prepared using ethanol and aqueous solvents. Proximate analysis was used to evaluate moisture content, ash values, crude fibers, and extractive values. Following that, preliminary phytochemical screening was done on both ethanol leaves extracts, and subsequently total flavonoid content was determined. In addition, TLC chromatograms and RP-HPLC studies were performed on both plant extracts to determine the presence of flavonoid components in…
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  • Genome-Wide Characterization of the Cellulose Synthase Gene Superfamily in Tea Plants (Camellia sinensis)
  • Abstract The cellulose synthase gene superfamily, including Cellulose synthase A (CesA) and cellulose synthase-like (Csl) gene families, is responsible for the synthesis of cellulose and hemicellulose, respectively. The CesA/Csl genes are vital for abiotic stress resistance and shoot tenderness regulation of tea plants (Camellia sinensis). However, the CesA/Csl gene family has not been extensively studied in tea plants. Here, we identified 53 CsCesA/Csl genes in tea plants. These genes were grouped into five subfamilies (CsCesA, CsCslB, CsCslD, CsCslE, CsCslG) based on the phylogenetic relationships with Arabidopsis and rice. The analysis of chromosome distribution, gene structure, protein domain and motif revealed that…
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  • Spatial Heterogeneity of Selected Soil Nutrients Related to Torreya grandis cv. Merrillii Plantation in Southeastern China
  • Abstract Chinese Torreya grandis (Torreya grandis cv. Merrillii) is a unique economic tree species in China. Intensive management related to application of chemical fertilizer and herbicides caused serious soil quality degradation of Chinese Torreya grandis plantations. Totally, 120 soil samples were collected from the main disbtributed areas of Chinese Torreya grandis in Southeastern China. In this area, soil pH values varied from 3.68 to 6.78, with a median value of 4.91, implying a trend of acidification. The average concentrations of organic matter, available nitrogen, available phosphorus and available potassium were 27.52 g kg−1, 135.77 mg kg−1, 15.12 mg kg−1, and 153.43…
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  • N-Exponential Fertilization Could Affect the Growth and Nitrogen Accumulation of Metasequoia glyptostroboides Seedling in a Greenhouse Environment
  • Abstract Metasequoia glyptostroboides (M. glyptostroboides) is a unique plant species related to relic flora in China. It plays a positive role in afforestation and its long-term protection with high paleoclimate research value. However, due to the nutrients-supply deficiency, it is a big challenge to cultivate the high-quality seedlings of M. glyptostroboides. In this study, a pot experiment in a greenhouse environment was carried out to identify the effect of N-exponential fertilization on the growth and nutrient distribution of M. glyptostroboides seedling. The M. glyptostroboides rooted seedlings with 12-month growth were chosen. Different N fertilizer levels with conventional fertilization (CF: 5.0 g…
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  • Identification of Suitable Reference Genes for qRT-PCR Normalization in Tilia miqueliana Maxim
  • Abstract Quantitative real-time polymerase chain reaction (qRT-PCR) is a rapid and effective approach toward detecting the expression patterns of target genes. The selection of a stable reference gene under specific test condition is essential for expressing levels of target genes accurately. Tilia miqueliana, considered endangered, is a prominent native ornamental and honey tree in East China. No study has evaluated the optimal endogenous reference gene for qRT-PCR analysis in T. miqueliana systematically. In this study, fifteen commonly used reference genes were selected as candidate genes, and the stabilities of their expressions were assessed using four algorithms (GeNorm, NormFiner, BestKeeper, and DeltaCt)…
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  • Transcriptome Analysis and Morphological Changes in Response to Waterlogging in Iris pseudacorus
  • Abstract Iris pseudacorus is a widely cultivated and studied ornamental plant with a large biomass, strong adaptability and extensive management. Moreover, it has the ability to decontaminate and enrich heavy metals. However, few studies have been conducted on its submergence tolerance with little known about the molecular response of I. pseudacorus to flooding. Morphologically, I. pseudacorus had strong adaptability to waterlogging, the aerenchyma was gradually enlarged and adventitious roots developed between 0 and 14 d. The transcriptome data showed that the differentially expressed genes counts in plants flooded for 2 h, 4 h, 12 h and 24 h compared with the…
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  • How Physical Disturbance and Nitrogen Addition Affect the Soil Carbon Decomposition?
  • Abstract The decomposition of soil organic carbon (SOC) plays a critical role in regulating atmospheric CO2 concentrations and climate dynamics. However, the mechanisms and factors controlling SOC decomposition are still not fully understood. Here, we conducted a 60 days incubation experiment to test the effects of physical disturbance and nitrogen (N) addition on SOC decomposition. N addition increased the concentration of NO3- by 51% in the soil, but had little effect on the concentration of NH4+. N addition inhibited SOC decomposition, but such an effect differed between disturbed and undisturbed soils. In disturbed and undisturbed soils, application of N decreased SOC…
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