Subhan Danish^1,2, Muhammad Zafar-ul-Hye^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 217-227, 2020, DOI:10.32604/phyton.2020.08523

Abstract Most of the cereal crops are widely cultivated to fulfil the humans food requirements. Under changing climate scenario, the intensity of drought stress is continuously increasing that is adversely affecting the growth and yield of cereal crops. Although the cereals can tolerate moderate drought to some extent, but mostly they are susceptible to severe drought stress. Higher biosynthesis of ethylene under drought stress has been reported. Many scientists observed that inoculation of 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing plant growth promoting rhizobacteria (PGPR) is an efficacious tool to overcome this problem. These PGPR secrete ACC deaminase which cleavage the ACC into the… More >

Fatih M. Tok¹, Sibel Dervis^2,*, Halit Yetisir³

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 303-314, 2020, DOI:10.32604/phyton.2020.08801

Abstract Forty-four V. dahliae isolates were collected from symptomatic vascular tissues of okra plants each from a different field in eight provinces located in the eastern Mediterranean and western Anatolia regions of Turkey during 2006- 2009. Nitrate-nonutilizing (nit) mutants of V. dahliae from okra were used to determine heterokaryosis and genetic relatedness among isolates. All isolates from okra plants were grouped into two vegetative compatibility groups (VCGs) (1 and 2) and three subgroups as 1A (13.6%, 6/44), 2A (20.5%, 9/44) and 2B (65.9%, 29/44) according to international criteria. Pathogenicity tests were performed on a susceptible local okra (A. esculentus) landrace in… More >

Muhammad Zafar-ul-Hye^1,*, Misbah Batool Zahra¹, Subhan Danish¹, Mazhar Abbas², Abdur Rehim¹, Muhammad Naeem Akbar¹, Ayesha Iftikhar¹, Mehreen Gul¹, Ifat Nazir¹, Maria Abid¹, Muhammad Tahzeeb-ul-Hassan¹, Maria Murtaza³

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 405-413, 2020, DOI:10.32604/phyton.2020.08918

Abstract Rhizosphere bacteria that colonize plant roots and confer beneficial effects are referred as plant growth promoting rhizobacteria (PGPR). Among all PGPR, some rhizobacteria have an ability to produce ACC deaminase enzyme. This enzyme catalyzes stress ACC into a-ketobutyrate and ammonia instead of letting it to be converted to ethylene. Ethylene level rises in plants under stress conditions i.e., drought, salinity, poor soil fertility etc. As poor soil fertility is a big hurdle to achieve the optimum yield of crops, inoculation of ACC deaminase PGPR can overcome this problem to some extent. The aim of the current study was to examine… More >

Yong Liang¹, Yuexing Chen², Dong Liu¹, Jianping Cheng³, Gang Zhao¹, Tzion Fahima⁴, Jun Yan^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 423-435, 2020, DOI:10.32604/phyton.2020.09339

Abstract Mineral nutrient malnutrition, especially deficiency in selenium (Se), affects the health of approximately 1 billion people worldwide. Wheat, a staple food crop, plays an important role in producing Se-enriched foodstuffs to increase the Se intake of humans. This study aimed to evaluate the effects of different Se application methods on grain yield and nutritional quality, grain Se absorption and accumulation, as well as 14 other trace elements concentrations in wheat grains. A sand culture experiment was conducted via a completely randomized 3 × 2 × 1 factorial scheme (three Se levels × two methods of Se application, foliar or soil… More >

Xiaohong Yin, Zui Tao, Jiana Chen, Fangbo Cao, Min Huang^*, Yingbin Zou

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 415-422, 2020, DOI:10.32604/phyton.2020.08962

Abstract It has been shown that adding biochar to soil can improve nitrogen (N) uptake and utilization in rice (Oryza sativa L.). However, there is a lack of research on the physiological alterations of rice as a result of the changes in nitrogen uptake due to the addition of biochar. This study conducted field experiments in 2015 and 2016 with the goal of testing the hypothesis that the application of biochar would enhance radiation use efficiency (RUE) of rice by improving the plant’s ability to take in and utilize nitrogen. Our results demonstrated that the application of biochar (20 t ha⁻¹… More >

Lu Zhu, Qiuyue Ma, Shushun Li, Jing Wen, Kunyuan Yan, Qianzhong Li^*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 385-403, 2020, DOI:10.32604/phyton.2020.09259

Abstract Quantitative real-time reverse transcriptase PCR (qRT-PCR) technology has been extensively used to estimate gene expression levels, and the selection of appropriate reference genes for qRT-PCR analysis is critically important for obtaining authentic normalized data. Acer palmatum is an important colorful leaf ornamental tree species, and reference genes suitable for normalization of the qRT-PCR data obtained from this species have not been investigated. In this study, the expression stability of ten candidate reference genes, namely, Actin3, Actin6, Actin9, EF1α, PP2A, SAMDC, TIP41, TUBα, TUBβ and UBQ10, in two distinct tissues (leaves and roots) of A. palmatum under four different abiotic stress… More >

Yuhao Weng¹, Liming Zhu², Yan Ma³, Hao Su¹, Lu Lu¹, Pengkai Wang¹, Jinhui Chen¹, Asif Ali¹, Renhua Zheng^4,*, Jisen Shi^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 375-384, 2020, DOI:10.32604/phyton.2020.08831

Abstract Cerasus Campanulata is one of several species belonging to the Prunoideae focke, a subfamily of the flowering plant Rosaceae. We investigated the details of its chloroplast genome which may reveal its genus independent of morphological determination. Here, we determined the complete chloroplast (cp) genome sequence of C. campanulata and performed sequence analysis to reveal the presence of 18 forward repeats, 20 palindrome repeats, 2 complement repeats, 4 reverse repeats and 93 simple sequence repeats (SSRs). We additionally performed a comparative study of C. campanulata and seven other Prunoideae focke species. Then, maximum parsimony (MP) and maximum likelihood (ML) phylogenetic analyses… More >

Gerardo Montelongo-Ruíz¹, Yolanda del Rocio Moreno-Ramírez¹, María Cruz Juárez-Aragón¹, Nohemí Niño-García², Reyna Ivonne Torres-Acosta², Torres-Castillo Jorge Ariel^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 361-373, 2020, DOI:10.32604/phyton.2020.08499

Abstract This work aims to determine the phytochemical characterization of the pericarp of Chamaedorea radicalis Mart. fruit as a non-timber product with potential to obtain phytochemicals with potential applications in the industry. Fruit from C. radicalis were grouped in four ripening stages named as S1, S2, S3 and S4, according to maturity; S1 the most unripe stage and S4 the completely ripe stage. Determinations of total phenolic compounds, free radical scavenging activities and total flavonoid contents using spectrophotometric methods were done. Also, the tentative identification of phytochemicals during fruit ripening was done using UPLC-MS-MS. Total phenolic compound (TPC) content ranged from… More >

María Laura Molinelli^*, Rocío Tarifa, Patricia Perissé

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 345-360, 2020, DOI:10.32604/phyton.2020.08796

Abstract Echium plantagineum (Boraginaceae) is native of the Mediterranean regions, has been introduced and become widespread within the American continent (North to South), South Africa, New Zealand and Australia. This plant has seed dormancy, aggressively spreads to infest vast areas of predominantly agricultural land and is considered a toxic weed to livestock. The objectives of this research were: (i) to study the morpho-anatomy of the diaspores of E. plantagineum; (ii) to identify the pathway of water uptake; and (iii) to characterize the germination and the seedling. The morpho-anatomical studies were carried out analyzing semi-permanent slides of transverse and longitudinal sections of… More >

Gang Li¹, Yao Zhou², Yaqi Zhao², Yaxue Liu², Yuwei Ke², Xiaoqing Jin¹, Haoli Ma^1,2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 329-344, 2020, DOI:10.32604/phyton.2020.08874

Abstract Quantitative real-time PCR (qRT-PCR) is widely used for investigating gene expression patterns and has many advantages, including its high sensitivity, fidelity, and specificity. Selecting a satisfactory internal reference gene is crucial for obtaining precise gene expression results in qRT-PCR analyses. In this study, the transcriptomic data of 2 potato varieties were screened for housekeeping genes with stable expression patterns. A total of 77 putative genes were selected, which were highly and stably expressed. Then, qRT-PCR analyses were performed to examine the expression levels of these 77 candidate reference genes in various potato tissues, including leaves, flowers, stolons, and tubers. Gene… More >