Torres, YA¹, MA Long², SM Zalba¹

Phyton-International Journal of Experimental Botany, Vol.77, pp. 151-160, 2008, DOI:10.32604/phyton.2008.77.151

Abstract Pavonia cymbalaria A. St.-Hil. & Naudin (Malvaceae) is a native species of the southern mountains in the Province of Buenos Aires. Its morphological characteristics become it as a potential candidate for ornamental use. Crop requirements and seed germination were evaluated for this species in this study. Different treatments were applied to 640 mericarps: removal of carpel residues, mechanical scarification or exposition of mericarps and nude seeds to cold conditions. An untreated control was used for each of these treatments. Germination only occurred on nude seeds; this implies the existence of an extrinsic dormancy imposed by the harsh mericarp which covers… More >

Giulietti¹ AL, OM Ruiz¹, HE Pedranzani¹, O Terenti^1,2

Phyton-International Journal of Experimental Botany, Vol.77, pp. 137-149, 2008, DOI:10.32604/phyton.2008.77.137

Abstract In this paper, the effects of four vermicomposts are analyzed on a variety of growth and development parameters of Digitaria eriantha cv. Mejorada INTA. The vermicomposts used originated from cow, horse, goat and hen feaces combined with pure soil in the following proportions: T1: 0% worm humus + 100% soil; T2: 50% worm humus + 50% soil, and T3: 100% worm humus + 0% soil. Digitaria eriantha seeds were sown in trays which were kept under controlled light and moisture conditions. After the third week of treatment, the number of germinated seeds, and both leaf and root length and dry… More >

Ávila-Quezada^1* G, E Sánchez¹, E Muñoz¹, LR Martínez², E Villalobos²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 129-136, 2008, DOI:10.32604/phyton.2008.77.129

Abstract We determined the microbiological quality of the most important fruits and vegetables in Chihuahua State (Mexico) to know the potential risks and apply prevention strategies prior to their consumption. Samples of jalapeño, bell and serrano pepper, cantaloupe, saladet and grape tomato, peach and Golden and Starkimson apple and of well, channel, irrigation and drinking water, as well as that for washing in packing were analyzed to determine Salmonella spp., Escherichia coli, total and fecal coliforms, fungi and yeasts. Determinations were made following the methodology indicated by the Mexican regulations (NOM-111-SSA1-1994). Sampling was made in the whole Chihuahua State during the… More >

Foroughbakhch R, RJ Ferry Sr, JL Hernández-Piñero, MA Alvarado-Vázquez, A Rocha-Estrada

Phyton-International Journal of Experimental Botany, Vol.77, pp. 113-127, 2008, DOI:10.32604/phyton.2008.77.113

Abstract Orchids of the genus Stanhopea are currently identified only by their floral structure characteristics. A statistical analysis of a significant number of species of this genus disclosed that measurements of adaxial and abaxial epidermal cell surface areas can be correlated with specific recognized species by a leaf printing method. This allows an objectively either positive or nearly positive confirmation of the identity of a species in the absence of flowers and without damage to plants. When ordering the mean values obtained for these surface areas in each species in a decreasing order, a correlation was observed in a hierarchical way… More >

Pérez-Amador¹ MC, V Muñoz Ocotero¹, F García Jiménez²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 103-111, 2008, DOI:10.32604/phyton.2008.77.103

Abstract Phototoxic compounds (polyacetylenes and thiophenes) and their biological activity were determined on Bidens squarrosa plants by measuring their antibacterial and anti-inflammatory potential. Phototoxic compounds were extracted with hexane, characterized by TLC, and their presence confirmed by U.V. spectrum of their extracts and bactericidal potential. Antibacterial activity of the hexane, ethyl acetate and methanol extracts was measured against Bacillus subtilis and Escherichia coli, and their antiinflammatory potential was determined by the mouse ear edema test. More >

Torroba MC, HA Paccapelo, L Aguilera, J Mazzola

Phyton-International Journal of Experimental Botany, Vol.77, pp. 93-102, 2008, DOI:10.32604/phyton.2008.77.093

Abstract An optimized methodology for improving regeneration of maize plants by direct organogenesis was evaluated. Our objective was to embarobtain genetically homogeneous plants through in vitro methods to regenerate clumps of multiple shoots from shoot tips at high frequency. Cultures were initiated from shoot tips of experimental lines of maize on a Murashige & Skoog (MS) medium supplemented with 2 mg/l benziladenine. Two experimental forage maize lines were used, in which two frequencies of subcultures were evaluated, namely line L. 850 (every 15 or 30 days) and line L.769 (every 30 days). It was observed that the highest rate of regeneration… More >

Azuara Hernández¹ L, H Silos Espino¹, C Perales Segovia¹, JF Gómez Leyva², AG Alpuche Solís³, LM Macias Valdez⁴

Phyton-International Journal of Experimental Botany, Vol.77, pp. 81-91, 2008, DOI:10.32604/phyton.2008.77.081

Abstract The objective of this study was to compare the yield and genetic relationships between two Perla garlic selections, obtained by individual selection of cloves, and commercial varieties and cultivars adapted to diverse regions of Mexico (which did not have a selection process). Varieties with fewer cloves showed higher yields. Coreano and California produced 20 ton/ha (fresh weight) and Perla selections between 17-19 ton/ha (fresh weight). Six primers of the series OPB were tested for genetic characterization and OPB-17 was selected. With the amplified DNA fragments, a bina-ry matrix was generated; afterwards, a dissimilarity matrix and dendrogram were developed. High genetic… More >

Giménez¹ C, E de García², O Haddad³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with… More >

Menéndez-Yuffá¹ A, L Ríos-Bolívar²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a characteristic set of protein… More >

Aguirre-Medina¹ JF, J Kohashi-Shibata², J Cadena-Iñiguez², C Avendaño-Arrazate²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 31-48, 2008, DOI:10.32604/phyton.2008.77.031

Abstract Intermittent drought is the main limiting factor for bean production in Mexico. The root system of three bean varieties of different origin and tolerance to water stress was exposed to different drying soil rates. The synthetic varieties were: Pinto Villa (PV, water stress tolerant) and Bayo Madero (BM, water stress susceptible) both of mesoamerican origin and growth habit type III (climbing habit), and ICA Palmar (water stress tolerant) of Andean origin and growth habit type I (shrub). Treatments were three soil drying rates, which were achieved by stopping irrigation and either maintaining or removing the pot cover made with PVC… More >