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- Evaluation on genetic relationships among China’s endemic Curcuma L. herbs by mtDNA
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 156-161, 2018, DOI:10.32604/phyton.2018.87.156
- Abstract Six mitochondrial (mtDNA) markers, cox2, ccmFn, ccb256, cox3, Nad4L-orf25, and atp4, were combined to assess genetic relationships by using Maximum Likelihood (ML) and Maximum parsimony (MP). This was done among nine Chinese Curcuma herbal species, included two rare species which are difficult to distinguish from their morphological characters. The relationships are very close among the nine species, and the overall distance was 0.018. In this study, the backbone of such nine species was achieved firstly to date, and being divided into two groups with moderate to strong bootstrap support. Both the ML and MP tree were obtained with similarity topologies…
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- Responses of leaf δ13C and leaf traits to precipitation and temperature in arid ecosystem of northwestern China
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 144-155, 2018, DOI:10.32604/phyton.2018.87.144
- Abstract Leaf δ13C is widely used to explain plant strategies related to resource availability in different environments. However, the coupled response of leaf δ13C to precipitation and temperature as well as the relationship between leaf δ13C and leaf traits remain unclear. The leaf δ13C and its relationship with leaf traits [leaf size (LS), leaf length (LL), leaf width (LW), leaf length to width ratio (L:W), specific leaf area (SLA) and mass-based leaf nitrogen concentration (Nmass)] were investigated on the dominant shrub species Nitraria tangutorum Bobr. in the arid region (Dengkou and Minqin) of northwestern China under the simulated increasing precipitation (PGS)…
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- Physiological response of early and late maturity oilseed rape cultivars to drought under two climate conditions
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 133-142, 2018, DOI:10.32604/phyton.2018.87.133
- Abstract Two experiments were performed in 2011-2012 to investigate the response of three oilseed rape cultivars to drought stress, grown under two climate conditions in Iran (cold and hot climates). The experiments were conducted using a randomized complete-block design arranged in split-plot with three replicates. The irrigation treatments (FI: full irrigation; WIF: withholding irrigation at flowering stage, and WIS: withholding irrigation at the silique formation stage until physiological maturity) were allocated to main plots, whereas subplots consisted of the oilseed rape cultivars: early maturating (GKH2005), relatively late maturing (Opera) and late maturing (Okapi). Drought caused a significant reduction in seed number,…
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- Responses of C:N:P stoichiometry of plants from a Hulunbuir grassland to salt stress, drought and nitrogen addition
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 123-132, 2018, DOI:10.32604/phyton.2018.87.123
- Abstract Chemical elements, such as carbon (C), nitrogen (N) and phosphorus (P), are major limiting nutrients in arid and semiarid grasslands and their stoichiometry (C:N:P) is a very important ratio to determine. In addition, it is critical to understand how plant stoichiometry responds to multiple environmental factors at the species level. In this study, we conducted a greenhouse experiment to investigate the effects of salt stress (4 g NaCl/kg soil), drought (35% of the soil water holding capacity) and N addition (10 g N/m2), as well as their interactions, on C, N and P concentrations and C:N:P ratios in two dominant…
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- Utility of taxonomic unit and life form in the analysis of plant diversity patterns in a temperate meadow steppe, China
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 113-122, 2018, DOI:10.32604/phyton.2018.87.113
- Abstract Rapid and precise assessment of biodiversity is a central issue in conservation biology and biodiversity science. Higher taxonomic units and life form-based morphospecies were utilised to assess plant diversity patterns at the habitat scale in temperate meadow steppe, Songnen Plain, China. Patch area, taxonomic composition (families and genera) and life form (based on Raunkiaer’s life forms, and stem and root growth forms) were recorded in five communities. Taxonomic unit-based hierarchical diversity indices (HDI) and life form-based hierarchical diversity indices (HLDI) were calculated. Twenty-six families, 67 genera, and 87 species were recorded in the five communities. HDI and HLDI were significantly…
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- Somatic embryogenesis induction from immature embryos of Sorghum bicolor L. (Moench)
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 105-112, 2018, DOI:10.32604/phyton.2018.87.105
- Abstract In this study, we established an in vitro regeneration system from immature embryos of four varieties of Sweet Sorghum with biotechnological potential. A total of 17 treatments were assesses in the induction of somatic embryogenic calli. We analyzed the effect of Murashige y Skoog media supplemented with different concentrations of plant growth regulators such as auxins and cytokinins. The callus induction response was evaluated using three immature embryos development stages and based on the number of embryos with callus induction. Different responses were observed in the embryos depending of the embryo age, culture condition, media composition and variety. The results…
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- Protocol for the reduction of costs in habanero chili (Capsicum chinense Jacq.) micropropagation
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 94-104, 2018, DOI:10.32604/phyton.2018.87.094
- Abstract An alternative method for improving the production of habanero chili is the tissue culture technique; however, the gelling agent, the high salt and sucrose concentrations used in the culture media raise production costs and limit the adaptation of the regenerated plants to greenhouse or field conditions. In this study, the effect of the substrates perlite-coconut fiber, coconut fiber-volcanic rock, vermiculite-perlite, and perlite-volcanic rock in conjunction with various culture media in in vitro plant regeneration from embryos was evaluated. The differentiation of adventitious shoots on substrates was scarcely observed or non-existent. Inducing the formation of shoots on agar and their development…
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- Molecular differentiation of Xoconostle (Opuntia spp.) using AFLP molecular markers
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087
- Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged…
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- Potential antioxidant and toxicological activity of the essential oil of Rhaphiodon echinus (Nees & Mart) Schauer (Lamiaceae): morphoanatomy and polyphenolic composition of its extracts
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 79-86, 2018, DOI:10.32604/phyton.2018.87.079
- Abstract The species Rhaphiodon echinus, known as betonica or creeping mint, is considered an infesting plant species, typical of the caatinga biome. Morphoanatomy is a very important tool to study the structure of plants, both its external and internal morphology, opening the knowledge about the production of secondary metabolites. These compounds are of great importance for scientific research, which may present themselves as the best antioxidants. Taking into account a lack of data in the literature on the essential oil of R. echinus, this study aimed to demonstrate its antioxidant activity and toxicity. In order to test this hypothesis, tests were…
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- Molecular biology techniques as new alternatives for medicinal plant identification
- Phyton-International Journal of Experimental Botany, Vol.87, pp. 72-78, 2018, DOI:10.32604/phyton.2018.87.072
- Abstract Mexico bears witness to a long-standing tradition in the use of plants for medicinal purposes; nevertheless, standardized methodological approaches for the proper identification of these are still needed. The problem is especially noticeable during the validation of ingredients in herbal preparations, as so many of them are sold as powders. It is for these reasons that the present study aims to compare the results of classic taxonomical assessments with those obtained using more modern molecular techniques (e.g. PCR-amplified 18S ribosomal RNA gene analysis) in one ethnobotanical case-study carried out in the “Desierto de los Leones” National Park in Mexico City,…
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