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  • Open Access

    ARTICLE

    Rapid Identification of a Candidate Gene Related to Fiber Strength Using a Superior Chromosome Segment Substitution Line from Gossypium hirsutum × Gossypium barbadense via Bulked Segregant RNA-Sequencing

    Qi Zhang1,#, Pengtao Li2,#, Aiying Liu1, Shaoqi Li1, Quanwei Lu2, Qun Ge1, Junwen Li1, Wankui Gong1, Xiaoying Deng1, Haihong Shang1,3, Yuzhen Shi1,*, Youlu Yuan1,3,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014437

    Abstract Cotton is the most widely cultivated commercial crop producing natural fiber around the world. As a critical trait for fiber quality, fiber strength principally determined during the secondary wall thickening period. Based on the developed BC5F3:5 CSSLs (chromosome segment substitution lines) from Gossypium hirsutum CCRI36 × G. barbadense Hai 1, the superior MBI9915 was chosen to construct the secondary segregated population BC7F2 with its recurrent parent CCRI36, which was subsequently subjected to Bulk segregant RNA-sequencing (BSR-seq) for rapid identification of candidate genes related to fiber strength. A total of 4 fiber-transcriptome libraries were separately constructed and sequenced, including two parents… More >

  • Open Access

    ARTICLE

    GC-MS Analysis of Metabolites in Filling Grains of Rice-Tartary Buckwheat (Fagopyrum tataricum) in Comparison to Conventional Tartary Buckwheat

    Chenggang Liang1,#, Chunyu Wei1,#, Yan Wang1,*, Wujuan Yu1, Kai Liao1, Taoxiong Shi1, Dabing Xiang2,3, Qijiao Chen1

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014242

    Abstract Rice-Tartary buckwheat (RTB) is a special germplasm of Tartary buckwheat. In this study, the appraisal of taste quality between RTB and conventional Tartary buckwheat (CTB) was presented, and the metabolites in kernels at three typical grain filling stage (GFS) were investigated. Unlike CTB, RTB showed thin shell seeds without longitudinal furrows at maturity, which was easily artificially dehulled. Sense organ test indicated that RTB exhibited better taste quality because of the higher values of appearance, viscosity, taste and summary were appraised. In total, 92 metabolites were identified in kernels using GM-MS metabolomics platform. The levels of most metabolites changed greatly… More >

  • Open Access

    ARTICLE

    Rapid Propagation of Rhynchostylis retusa in Vitro

    Yinkai Xi1,2, Biao Zeng3, Hengyu Huang1,4,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014218

    Abstract An efficient regeneration system of Rhynchostylis retusa was established to provide technical reference for the application of tissue culture tube seedlings in production. The mixtures of callus and protocorm from aseptic germination were used as explants. The optimal media of each stage was selected for callus proliferation, protocorm occurrence and growth, rejuvenation and rooting via a single, complete combination and orthogonal experiment. The results showed that the optimal medium for callus proliferation, protocorms occurrence and growth was 1/2 Murashige and Skoog (MS) medium adding 50 g·L−1 banana puree, 0.1 mg·L−1 α-naphthaleneacetic acid (NAA), 1.5 mg·L−1 6-benzylaminopurine (6-BA) and 1.0 mg·L−1More >

  • Open Access

    ARTICLE

    Effects of Different Potassium (K) Fertilizer Rates on Yield Formation and Lodging of Rice

    Tantan Zhang1,2,#, Xiaoping He3,#, Binglin Chen1,#, Longxin He1,2, Xiangru Tang1,2,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014168

    Abstract As one of the most important nutrients for plants, potassium (K) has substantial effects on growth and development of crops. Present study was conducted in three different sites in South China in late season in 2019 with the objective to study the effects of different applied amounts of K fertilizer on yield formation and lodging of rice. Four K fertilizer treatments, K0: 0 kg potassium oxide (K2O) ha−1 (control); K1: 64.20 kg K2O ha−1 ; K2: 128.55 kg K2O ha−1 and K3 153.90 kg K2O ha−1 were applied in the field experiment. The results showed that K2 and K3 treatments… More >

  • Open Access

    ARTICLE

    In Vitro Propagation of Agave guiengola Gentry Using Semisolid Medium and Temporary Immersion Bioreactors

    Lucía Isabel Chávez-Ortiz1, José Francisco Morales-Domínguez1, Araceli Rodríguez-Sahagún2, Eugenio Pérez-Molphe-Balch1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.012862

    Abstract Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l–1 BA, obtaining a mean… More >

  • Open Access

    ARTICLE

    Mycorrhizal Networks Interacting with Litter Improves Nutrients and Growth for One Plant through the Vary of N/P Ratio under Karst Soil

    Liling Kang1, Yuejun He1,*, Lipeng Zang1, Jianpeng Si1, Ying Yang1, Kaiping Shen1, Tingting Xia1, Qiyu Tan1, Bangli Wu1, Yun Guo1, Wei Wang2, Qin Liang3

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014740

    Abstract Arbuscular mycorrhizae (AM) fungi affect nutrient uptake for host plants, while it is unclear how AM fungi interacting with soil litter affect plant growth and nutrient utilization through mycorrhizal networks in karst soil of deficient nutrients beyond the rhizosphere. An experiment was conducted in a microcosm composed of a planting compartment for Cinnamomum camphora seedlings with or without Glomus mosseae fungus (M+ vs. M ) and an adjacent litter compartment containing or not containing additional litter material of Arthraxon hispidus (L+ vs. L ), where the compartments are connected either by nylon mesh of 20 μm or 0.45 μm which… More >

  • Open Access

    ARTICLE

    Study of Spectral Response Characteristics of Oilseed Rape (Brassica napus) to Particulate Matters Based on Hyper-Spectral Technique

    Lijuan Kong1,2, Haiye Yu1,2, Zhaojia Piao1,2, Meichen Chen1,2, Jingmin Dang1, Lei Zhang1,2, Yuanyuan Sui1,2,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014190

    Abstract Haze is mainly caused by the suspended particulate matters in the air, of which the particulate matters pollution harms leaf vegetables. In this paper, oilseed rapes at four different growing periods were investigated in a simulated particulate pollution environment. In combination of hyper-spectral technology and micro examination, the response of hyper-spectral characteristics of the leaf to particulate matters was investigated in-depth. The hyperspectral, chlorophyll content, net photosynthetic rate and stomatal conductance of leaf were obtained. The deposition and adsorption of particulate matters on the leaf were observed by Environmental Scanning Electron Microscope (ESEM). Normalized difference vegetation index (NDVI), modified red… More >

  • Open Access

    ARTICLE

    An Efficient Plant Regeneration System of Hydrangea bretschneideri Dipp via Stem Segments as Explants

    Si ran Wang#, Jia Yan#, Bu er Ha, Yu’e Bai*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.013693

    Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL−1 6-BA and 0.5 mgL−1 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation coefficient… More >

  • Open Access

    ARTICLE

    Ameliorative Role of Pre-Sowing Proline Treatment in Coriandrum sativum L. Seedlings under Mercury Toxicity

    Dhriti Kapoor1, Kapani Kavani1, Amandeep Rattan2, Marco Landi3,*, Anket Sharma4,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.012359

    Abstract Heavy metal toxicity is one of the major ecosystem concerns globally in present time and is also responsible for significant threat to agronomic crops. The current work was conducted to investigate the possible ameliorative role of proline in Coriandrum sativum L. seedlings treated with mercury (Hg). The seedlings were exposed to different concentrations of Hg (0, 0.1, 0.3 and 0.5 mM) for 20 days. The effects of pre-sowing treatment with proline were studied on C. sativum seedlings in terms of pigment (chlorophylls, carotenoids and anthocyanins), malondialdehyde (MDA), antioxidant compound (glutathione, total phenolic compounds, ascorbic acid) and osmolytes (proline, glycine betaine).… More >

  • Open Access

    ARTICLE

    Interaction of Acaena elongataL. with Arbuscular Mycorrhizal Fungi under Phosphorus Limitation Conditions in a Temperate Forest

    Yasmin Vázquez-Santos1, Yuriana Martínez-Orea1, Javier Álvarez-Sánchez1, Noé Manuel Montaño2, Sara Lucía Camargo-Ricalde2, Silvia Castillo-Argüero1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2021.014208

    Abstract The aim of this study was to analyze the performance of Acaena elongata colonized by arbuscular mycorrhizal fungi (AMF) to different phosphorus (P) concentrations, as a measure of AMF dependency. A. elongata, is a species from soils where P availability is limited, such as temperate forests. Our research questions were: 1) How do different P concentrations affect the AMF association in Acaena elongata, and 2) How does the AMF association influence A. elongata’s growth under different P concentrations? A. elongata’s growth, P content in plant tissue, AMF colonization and dependency were measured under four P concentrations: control (0 g P… More >

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