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  • Grapevine Phenological Quantitative Trait SSR Genotyping Using High-Throughput HRM-PCR Analysis
  • Abstract Discrimination among grapevine varieties based on quantitative traits, such as flowering, veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs. These traits are under complex genetic control for which 6 linked SSR loci (VVS2, VVIn16, VMC7G3, VrZAG29, VMC5G7, and VVIB23) have been identified. Using these markers in HRM-PCR analysis, we assessed genetic diversity among a large collection of 192 grapevine varieties. The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties, 3 prominent foreign varieties and 11 varieties of Palestinian origin. The SSR markers used were highly…
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  • Comparative Analyses and Phylogenetic Relationships between Cryptomeria fortunei and Related Species Based on Complete Chloroplast Genomes
  • Abstract Cryptomeria fortunei (Chinese cedar) is a highly adaptable woody species and one of the main forest plantation trees in subtropical high-altitude areas in China. However, there are few studies on its chloroplast (cp) genome. In this study, the complete cp genome of C. fortunei was sequenced and evaluated via comparative analyses with those of related species (formerly the Taxodiaceae) in Cupressaceae. The C. fortunei cp genome was 131,580 bp in length, and the GC content of the whole genome was 35.38%. It lost one relevant large inverted repeat and contained 114 unique genes, including 82 protein-coding genes, 28 tRNAs and…
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  • Characterization and Expression of Ammonium Transporter in Peach (Prunus persica) and Regulation Analysis in Response to External Ammonium Supply
  • Abstract As the preferred nitrogen (N) source, ammonium (NH4+ ) contributes to plant growth and development and fruit quality. In plants, NH4+ uptake is facilitated by a family of NH4+ transporters (AMT). However, the molecular mechanisms and functional characteristics of the AMT genes in peach have not been mentioned yet. In this present study, excess NH4+ stress severely hindered shoot growth and root elongation, accompanied with reduced mineral accumulation, decreased leaf chlorophyll concentration, and stunned photosynthetic performance. In addition, we identified 14 putative AMT genes in peach (PpeAMT). Expression analysis showed that PpeAMT genes were differently expressed in peach leaves, stems…
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  • 2,4-D Hyper Accumulation Induced Cellular Responses of Azolla pinnata R. Br. to Sustain Herbicidal Stress
  • Abstract In the present experiment with ongoing concentration (0 µM, 100 µM, 250 µM, 500 µM and 1000 µM) of 2,4-D, the responses of Azolla pinnata R.Br. was evaluated based on cellular functions. Initially, plants were significantly tolerated up to 1000 µM of 2,4-D with its survival. This was accompanied by a steady decline of indole acetic acid (IAA) concentration in tissues with 78.8% over the control. Membrane bound H+ -ATPase activity was over expressed within a range of 1.14 to 1.25 folds with activator (KCl) and decreased within a range of 57.3 to 74.6% in response to inhibitor (Vanadate) application.…
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  • Changes of the Flowering Time of Trees in Spring by Climate Change in Seoul, South Korea
  • Abstract Flowering onset has attracted much attention in ecological research as an important indicator of climate change. Generally, warmer temperatures advance flowering onset. The effect of climate warming on flowering onset is more pronounced in spring because the difference between atmospheric and water temperatures creates more rapid convection than in other seasons. We analyzed the correlation between 73 species of spring woody plants in Hongneung Arboretum in Seoul, South Korea and the spring minimum temperature and average precipitation over the past 50 years (1968–2018). The spring minimum temperature and average precipitation have increased over the past 50 years, resulting in the…
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  • Titanium Dioxide Nanoparticles Promote Root Growth by Interfering with Auxin Pathways in Arabidopsis thaliana
  • Abstract TiO2 nanoparticles (nano-TiO2) are widely used in the world, and a considerable amount of nano-TiO2 is released into the environment, with toxic effects on organisms. In the various species of higher plants, growth, including seed germination, root elongation, and biomass accumulation, is affected by nano-TiO2. However, the underlying molecular mechanisms remain to be elucidated. In this study, we observed that nano-TiO2 promoted root elongation in a dose-dependent manner. Furthermore, we found that nano-TiO2 elevated auxin accumulation in the root tips of the auxin marker lines DII-VENUS and DR5:: GUS, and, correspondingly, quantitative real-time PCR analysis revealed that nano-TiO2 increased the…
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  • Metabolic Profiling in Banana Pseudo-Stem Reveals a Diverse Set of Bioactive Compounds with Potential Nutritional and Industrial Applications
  • Abstract Banana (Musa spp.) is an ancient and popular fruit plant with highly nutritious fruit. The pseudo-stem of banana represents on average 75% of the total dry mass but its valorization as a nutritional and industrial by-product is limited. Recent advances in metabolomics have paved the way to understand and evaluate the presence of diverse sets of metabolites in different plant parts. This study aimed at exploring the diversity of primary and secondary metabolites in the banana pseudo-stem. Hereby, we identified and quantified 373 metabolites from a diverse range of classes including, alkaloids, flavonoids, lipids, phenolic acids, amino acids and its…
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  • Effects of Multi-Stage Continuous Drought on Photosynthetic Characteristics, Yield and Water Use Efficiency of Winter Wheat
  • Abstract A drought event can cause entire crops to fail or yield loss. In order to study the effects of continuous drought on photosynthetic characteristics, yield, and water use efficiency (WUE) of winter wheat (Triticum aestivum L.), the winter wheat variety “Aikang 58” was selected as test material with controlling the water of the pot-planted winter wheat under a mobile rainout shelter. Based on foot planting and safe wintering, winter wheat was evaluated under different drought conditions, including light, moderate and severe drought at the jointing (B), heading (C), and filling (G) stages. The soil water content was controlled in a…
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  • UVI_02019870, a Puptive Effector from Ustilaginoidea virens, Interacts with a Chloroplastic-Like Protein OsCPL1
  • Abstract Ustilaginoidea virens, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in U. virens often act as a group of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of individual effectors in U. virens need to be further explored. Here, we found a small secreted hypersensitive response-inducing protein UVI_02019870 was highly conserved in fungi. Furthermore, we performed Y2H and BiFC assay to demonstrated UVI_02019870 interacted with OsCPL1, which was predicted as a chloroplast…
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  • Plant Derived Antiviral Products for Potential Treatment of COVID-19: A Review
  • Abstract COVID-19 caused by SARS-CoV-2 is declared global pandemic. The virus owing high resemblance with SARS-CoV and MERS-CoV has been placed in family of beta-coronavirus. However, transmission and infectivity rate of COVID-19 is quite higher as compared to other members of family. Effective management strategy with potential drug availability will break the virus transmission chain subsequently reduce the pressure on the healthcare system. Extensive research trials are underway to develop novel efficient therapeutics against SARS-CoV-2. In this review, we have discussed the origin and family of coronavirus, structure, genome and pathogenesis of virus SARS-CoV-2 inside human host cell; comparison among SARS,…
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