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  • Open Access


    Diagnosis and Recommendation Integrated System Assessment of the Nutrients Limiting and Nutritional Status of Tomato

    Rabia Manzoor1,*, Mohammad Saleem Akhtar1, Khalid Saifullah Khan1, Taqi Raza2, Muhammad Ishaq Asif Rehmani3, Carl Rosen4, Muhammad Khalil ur Rehman5, Nahla Zidan6, Fahad M. Alzuaibr7, Nisreen M. Abdulsalam8, Najla A. Khateeb9, Majid Alhomrani10,11, Abdulhakeem S. Alamri10,11, Javeed Ahmad Lone12, Muhammad Ammar Raza13, Ayman El Sabag

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022988

    Abstract Tomato is an important field crop, and nutritional imbalances frequently reduce its yield. Diagnosis and Recommendation Integrated System (DRIS), uses ratios for nutrient deficiency diagnosis instead of absolute concentration in plant tests. In this study, local DRIS norms for the field tomatoes were established and the nutrient(s) limiting tomatoes yield were determined. Tomato leaves were analyzed for nutrients, to identify nutritional status using the DRIS approach. One hundred tomatoes fields were selected from Chatter Plain Khyber Pakhtunkhwa and the Sheikupura Punjab Pakistan. The first fully matured leaf was sampled, rinsed, dried and ground for analyzing P, K, Ca, Mg, Cu,… More >

  • Open Access


    Transcriptome Analysis and Physiological Responses of Economic Macroalga Gracilariopsis lemaneiformis under Sulfate Deficiency

    Haojie Jia1,2, Hong Du1,2,*, Muhmmad Aslam1,2, Tangcheng Li1,2, Canqi Zheng1,2, Xihui Xie1,2, Nan Huang1,2, Honghao Liang1,2, Pengbing Pei1,2, Ping Li1,2, Weizhou Chen1,2, Xiaojuan Liu1,2

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022663

    Abstract Sulfur is an essential macronutrient for the growth of all photosynthetic organisms and plays important roles in different metabolic pathways. However, sulfur metabolism and its related research on macroalgae with important ecological value is rather limited. In this study, marine ecological valued macroalga Gracilariopsis lemaneiformis was used to study the general physiological responses and transcriptome profiling under the sulfate deficiency. The relative growth rate of algae under sulfate deficiency was statistically significantly lower than that of control after 6 days. However, no significant differences were observed in the pigments content and Fv/Fm value, indicating that the photosynthesis was not obviously… More >

  • Open Access


    Do Geographically Isolated Grasslands Follow the Principle of Island Biogeography in a Landscape Scale? Taking Poyang Lake Grassland as an Example

    Shiqi Luo1,3, Wenbo Chen2,3,*, Lei He4, Qiongbing Xiong1,3

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022466

    Abstract As one of the basic theories of biodiversity conservation, island biogeography has been widely accepted in the past decades. Originally, island biogeography was put forward and applied in oceanic environments. But later on, it was found out that the application was not only limited to oceanic islands, but also in terrestrial environments with relatively isolated conditions. In terms of biodiversity level, island biogeography generally focuses on a small scale, such as species diversity and genetic diversity. The studies of biodiversity on a large-scale based on island biogeography, such as ecosystem and landscape scales, were seldomly conducted. Taking Poyang Lake, the… More >

  • Open Access


    Arbuscular Mycorrhizal Fungal Diversity Associated with Olea europaea L. Growing in Yunnan Province, Southwestern China

    Yuebo Jing*, Jihua Mao, Rongbo Li

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.021989

    Abstract Olive (Olea europaea L.) is one of the most important and widely cultivated fruit trees, with high economic, ecological, cultural and scientific value. China began introducing and cultivating olive in the 1960s, and Yunnan Province is one of the main growing areas. Improving the cultivation and productivity of this tree crop species is an important challenge. Olive is a typical mycotrophic species and the potential of arbuscular mycorrhizal fungi (AMF) for this plant is well recognized; nevertheless, studies of olive AMF in China are still very limited. Roots and rhizosphere soils of olive were sampled from five representative growing sites… More >

  • Open Access


    In vitro Evaluation of Seed Germination in Twelve Alfalfa Cultivars under Salt Stress

    Walid Soufan*, Yaser Hassan Dewir, Nasser A. Al-Suhaibani

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.023115

    Abstract Alfalfa (Medicago sativa L.), when exposed to abiotic stress such as salinity, suffers significant losses in yield and productivity. The present study evaluated the salinity tolerance of 12 alfalfa cultivars in vitro using five concentrations of sodium chloride (NaCl), ranging from 0 to 250 mmol L−1 . The results obtained in the current study revealed that the Saudi cultivars, Kasimi and Hassawi, and the German cultivar (Berlin) had the highest salinity tolerance in terms of germination percentage (GP), corrected germination rate index (CGRI), days to reach 50% germination (GT50), and ability to form cotyledonary and true leaves. Under mmol L−1More >

  • Open Access


    Observation on Flower Bud Differentiation of Crape Myrtle in Red Soil Environment

    Linxue Shang1,2,3,#, Dandan Ma4,#, Sidan Hong1,2,3,#, Yu Zhao1,2,3,#, Guozhe Zhang1,2,3,#, Qingqing Ma1,2,3, Qun Wang1,2,3,*, Cuihua Gu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022485

    Abstract Flower bud differentiation is a key component of plant blooming biology and understanding how it works is vital for flowering regulation and plant genetic breeding, increasing the number and quality of flowering. Red soil is the most widely covered soil type in the world, and it is also the most suitable soil type for crape myrtle planting. The flower buds of crape myrtle (Lagerstroemia indica) planted in red soil were employed as experimental materials in this study, and the distinct periods of differentiation were identified using stereomicroscopy and paraffin sectioning. We optimized the steps of dehydration, transparency, embedding, sectioning and… More >

  • Open Access


    Effects of Two Potential Allelochemicals on the Photosystem II of Nitzschia closterium and Monostroma nitidum

    Bowen Huang, Enyi Xie*, Yu Ran, Xinyi Chen, Yongjian Huang, Jianjun Cui*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022672

    Abstract In aquaculture, high-density seaweed farming brings higher economic benefits but also increases outbreaks of diatom felt. The effective control of diatom felt in high-density seaweed farming has always been a research hotspot. This study selected two potential allelochemicals 2-hydroxycinnamic acid and quinic acid to explore their effects on a diatom Nitzschia closterium and an economic seaweed Monostroma nitidum. The results showed that 2-hydroxycinnamic acid had better inhibitory effects than quinic acid on the growth, pigment content and photosynthetic efficiency of N. closterium. Their half-maximal inhibitory concentrations at 120 h (IC50–120 h) were 0.9000 and 1.278 mM, respectively. Additionally, these allelochemicals… More >

  • Open Access


    The Endosperm-Specific Expression of YUCCA Genes Enhances Rice Grain Filling

    Huijun Jiang1,#, Kaien Zhai3,#, Xiaofan Ye3,#, Tianwei Hu2, Jieming Jiang4, Xiaoqiu Dong1, Weihuai Pan1, Jianwei Pan2, Jianxin Shou1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.021474

    Abstract Grain filling is a crucial process that affects yield in rice (Oryza sativa L.). Auxin biosynthesis and signaling are closely related to rice yield; therefore, it is important to understand the effects of auxin biosynthesis on rice grain filling to improve crop yield. In this study, we used physiological and molecular strategies to identify the roles of auxin in rice grain filling. Exogenous application of auxin (IAA) or auxin analogues (2, 4-D) to young spikelets and flag leaves improved the seed-setting rate and yield per spike. Furthermore, real-time quantitative PCR assays confirmed that nine members of the OsYUCCA family of… More >

  • Open Access


    GhSCL4 Acts as a Positive Regulator in Both Transgenic Arabidopsis and Cotton during Salt Stress

    Yanyan Zhao1,*, Yanpeng Ding2, Bailin Duan1, Qingzhou Xie1

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.022384

    Abstract GRAS transcription factors play important roles in plant abiotic stress response, but their characteristics and functions in cotton have not been fully investigated. A cotton SCL4/7 subgroup gene in the GRAS family, GhSCL4, was found to be induced by NaCl treatments. Nuclear localization and transactivation activity of GhSCL4 indicate its potential role in transcriptional regulation. Transgenic Arabidopsis thaliana over-expressing GhSCL4 showed enhanced resistance to salt and osmotic stress. What’s more, the transcript levels of salt stress-induced genes (AtNHX1 and AtSOS1) and oxidation-related genes (AtAPX3 and AtCSD2) were more highly induced in the GhSCL4 over-expression lines than in wild type after… More >

  • Open Access


    Identification and Characterization of a Novel Yellow Leaf Mutant yl1 in Rice

    Xiaofang Zeng1,#, Guangzheng Li1,#, Nu’an Liu2, Yan Li1, Jianrong Li1, Xiaozhen Huang1, Degang Zhao1,2,,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2022.021199

    Abstract Leaf-color mutants play an important role in the study of chlorophyll metabolism, chloroplast development, and photosynthesis system. In this study, the yellow leaf 1 (yl1) rice mutant was identified from the ethyl methane sulfonate-treated mutant progeny of Lailong, a glutinous japonica rice landrace cultivated in Guizhou Province, China. Results showed that yl1 exhibited yellow leaves with decreased chlorophyll content throughout the growth period. Chloroplast development in the yl1 mutant was disrupted, and the grana lamellae was loosely packed and disordered. RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) analysis revealed that the chlorophyll synthesis-related genes OsCHLH, OsCHLM, OsCHLG, PORB,… More >

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