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  • Open Access

    ARTICLE

    The Impact of Inoculum Preparation Media on Pollutant Removal through Phycoremediation of Agricultural Drainage Water by Desmodesmus sp.

    Asmaa Salah1, Hoda Sany1, Abo El-Khair B. El-Sayed2, Reham M. El-Bahbohy1, Heba I. Mohamed3,*, Ayman Amin1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.031064

    Abstract Water is the most essential natural resource for the future development. Agriculture production is extensively water-dependent and a significant polluter of water resources. So, this work investigated the effect of two different preparation media [Bold’s Basal Medium (BBM) and Domiati cheese whey (DCW)] for agricultural drainage water (ADW) remediation. All treatments were incubated for 6 days. According to the results of biomass productivity, specific growth rate, photosynthetic pigments, and biochemical composition, Desmodesmus sp. can grow in drainage water without dilution. The two treatments significantly reduced the concentration of nitrate, phosphate, chemical oxygen demand, and sodium in ADW. Finally, using cheese… More >

  • Open Access

    ARTICLE

    Comparison of Apigenin, Quercetin and Kaempferol Accumulation and Total Flavonoid Content in Leaves, Embryogenic Cultures and Cell Suspension Cultures of Parsley (Petroselinum crispum)

    Laura Isabel Arias-Rodríguez1, Martha Alicia Rodríguez-Mendiola2,*, Carlos Arias-Castro2,*, Federico Antonio Gutiérrez Miceli1, Diana Reséndez Pérez3, María Celina Luján Hidalgo1, Juan José Villalobos Maldonado1, Norma Alejandra Mancilla Margalli2

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.030396

    Abstract In recent years, there has been a growing interest in exploring alternative treatments for bone defects. Bone tissue engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of bone regeneration. In our study, we specifically investigated the extract of Petroselinum crispum, a plant known for its abundance of osteogenic flavonoids such as apigenin, quercetin, and kaempferol. Our objective was to compare the total flavonoid content (TFC) and their accumulation in different sources. We obtained hydrolyzed aqueous extracts from the leaves of parsley plants (grown for 12 weeks in the field), weekly embryogenic cultures, and suspension… More >

  • Open Access

    ARTICLE

    Appraisal of Improvement in Physiological and Metabolic Processes by Exogenously Applied Natural and Synthetic Ascorbic Acid in Okra (Abelmoschus esculentus L.) Fruit Subjected to Water Deficit Stress

    Muhammad Younis1, Nudrat Aisha Akram1,*, Arafat Abdel Hamed Abdel Latef2,*, Muhammad Ashraf3

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.028801

    Abstract To counteract the effects of drought stress, scientists have adopted several approaches including the use of different chemicals both inorganic and organic, which is contemplated as a highly efficient and cost-effective shot-gun approach. Ascorbic acid (AsA) is a potential organic substance, which widely occurs in plants, and is considered to be an effective antioxidant to counteract reactive oxygen species (ROS). Thus, a pot experiment was performed to assess the relative mitigating impacts of synthetic AsA and naturally occurring AsA in the form of lemon juice (LJ) and orange juice (OJ) on two cultivars of okra (Abelmoschus esculentus L.) namely Sabz… More >

  • Open Access

    REVIEW

    Molecular Mechanism Underlying Plant Response to Cold Stress

    Yiwei Cao, Delight Hwarari, Yasmina Radani, Yuanlin Guan, Liming Yang*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.024929

    Abstract Low temperature stress is one of the most important factors limiting plant growth and geographical distribution. In order to adapt to low temperature, plants have evolved strategies to acquire cold tolerance, known as, cold acclimation. Current molecular and genomic studies have reported that annual herbaceous and perennial woody plants share similar cold acclimation mechanisms. However, woody perennials also require extra resilience to survive cold winters. Thus, trees have acquired complex dynamic processes to control the development of dormancy and cold resistance, ensuring successful tolerance during the coldest winter season. In this review, we systemically described how woody plants perceive and… More >

  • Open Access

    REVIEW

    Breaking Barriers: Selenium and Silicon-Mediated Strategies for Mitigating Abiotic Stress in Plants

    Mojtaba Kordrostami1, Ali Akbar Ghasemi-Soloklui1, Mohammad Anwar Hossain2,*, Mohammad Golam Mostofa3,4,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.030372

    Abstract Numerous plant species, particularly those that can accumulate selenium (Se) and silicon (Si), benefit from these essential micronutrients. Se and Si accumulation in plants profoundly affects several biochemical reactions in cells. Understanding how plants react to Se/Si enrichment is crucial for ensuring adequate dietary Se/Si intake for humans and animals and increasing plant tolerance to environmental stressors. Several studies have shown that Se/Si-enriched plants are more resistant to salinity, drought, extreme temperatures, UV radiation, and excess metalloids. The interplay between Se/Si in plants is crucial for maintaining growth and development under normal conditions while providing a critical defense mechanism against… More >

  • Open Access

    ARTICLE

    Evaluation of Beta-Lactam Antibiotics on the Regeneration of Peanut Plants and Their Inhibitory Effect on Agrobacterium Growth

    Abraham Lamboro1,3,*, Songnan Yang1, Xueying Li1, Dan Yao2, Jun Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.029492

    Abstract The effect of beta-lactam antibiotics on shoot induction and plantlet regeneration from cotyledonary nodes was tested using two peanut cultivars. The culture media contained 4 mg/L 6-benzylaminopurine (BAP) as the main growth regulator. Various concentrations (100–600 mg/L) of cefotaxime, carbenicillin, and timentin were applied in the culture media. In all the tested media, there were no significant differences in the shoot induction as compared to the control. However, little phytotoxic effect was observed at higher concentrations of these antibiotics in the shoot elongation media. Under shoot elongation medium, shoots turned brownish and partly died at higher concentrations where shooting rates… More >

  • Open Access

    ARTICLE

    Tissue Culture of Calla Lily (Zantedeschia spreng.): An Updated Review on the Present Scenario and Future Prospects

    Xuan Sun1,2, Xue Wang1, Bijaya Sharma Subedi3, Yin Jiang1,2, Di Wang1,2, Rongxin Gou1,2, Guojun Zhang2, Wenting Xu4,*, Zunzheng Wei1,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.029667

    Abstract The calla lily (Zantedeschia spreng.) is a bulbous flower native to the tropical regions of Africa. Calla lily has gained significant popularity in the international market owing to its intricate morphology and prolonged flowering duration. Despite such advantages, for two sub-groups of calla lily, known as group Zantedeschia and group Aestivae, there are challenges in terms of hybrid production due to the ‘plastome-genome incompatibility’ therebetween. Tissue culture is a fundamental biotechnological tool used in gene editing research, with a focus on disease resistance and flower color in calla lily breeding programs. The present review provides a brief background on the… More >

  • Open Access

    ARTICLE

    Selenium: A Game Changer in Plant Development, Growth, and Stress Tolerance, via the Modulation in Gene Expression and Secondary Metabolite Biosynthesis

    Ali Bandehagh1,*, Zahra Dehghanian2, Vahideh Gougerdchi1, Mohammad Anwar Hossain3,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.028586

    Abstract The presence of selenium (Se) is not widely established as crucial for crops, although it is commonly recognized as an important nutrient for animals as well as humans. Even so, it is inevitably accepted that Se usually contributes positively to the life cycle of plants. Previous findings suggested that small amounts of Se seem to have a productive role in growth and production. As a result, Se is assumed to function in multiple ways, primarily by influencing a variety of biochemical and physiological functions. Also, Se also acts as a plant antioxidant and pro-oxidant and confers tolerance against different abiotic… More >

  • Open Access

    ARTICLE

    Cloning and Functional Validation of Mung Bean VrPR Gene

    Xiaokui Huang1, Yingbin Xue1, Aaqil Khan1, Hanqiao Hu1, Naijie Feng1,2,*, Dianfeng Zheng1,2,*

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.027457

    Abstract For the purpose of functional validation, the mung bean (Vigna radiata) VrPR gene was cloned and overexpressed in Arabidopsis thaliana. The findings revealed that the ORF of VrPR contained 1200 bp, in which 399 amino acids were encoded. Bioinformatics analysis showed that the VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins. VrPR was assumed to have 44 amino acid phosphorylation sites and be contained in chloroplasts. The VrPR secondary structure comprised of random coil, α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure.… More >

  • Open Access

    ARTICLE

    In silico Prediction and Analysis of Potential Off-Targets and Off-Target Mutation Detection in StERF3-Gene Edited Potato Plants

    Hafiza Arooj Razzaq1, Siddra Ijaz1,*, Imran Ul Haq2, Faisal Saeed Awan1

    Phyton-International Journal of Experimental Botany, Vol., , DOI:10.32604/phyton.2023.030501

    Abstract The imperative aspect of the CRISPR/Cas9 system is a short stretch of 20 nucleotides of gRNA that control the overall specificity. Due to the small size, the chance of its multiple occurrences in the genome increases; however, a few mismatches are tolerated by the Cas9 endonuclease activity. An accurate and careful in silico-based off-target prediction while target selection is preferred to address the issue. These predictions are based on a comprehensive set of selectable parameters. Therefore, we investigated the possible off-target prediction and their screening in StERF3 gene-edited potato plants while developing StERF3-loss-of-function mutants using CRISPR/Cas9 approach. The 201 off-targets… More >

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