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  • Open Access

    ARTICLE

    Intrahost distribution and trasmission of a new species of cyclopoid copepod endosymbiotic to a freshwater snail, Pomacea canaliculata (Caenogastropoda, Ampullariidae) from Argentina

    C. D. GAMARRA-LUQUES, I. A. VEGA, E. KOCH, A. CASTRO-VAZQUEZ

    BIOCELL, Vol.28, No.2, pp. 155-164, 2004, DOI:10.32604/biocell.2004.28.155

    Abstract A new species of cyclopoid copepod, Ozmana huarpium, is described as a symbiont to Pomacea canaliculata (Lamarck 1822) (Caenogastropoda, Ampullariidae). Rather large numbers (about one hundred copepods per snail) were found, although there was no evidence of harm to the host. To our knowledge, O. haemophila (symbiont to P. maculata), and the currently described species, O. huarpium, are the only copepod species ever recorded as endosymbionts to freshwater invertebrates. While O. haemophila is restricted to the haemocoel of its host, O. huarpium predominate in the penis sheath, the ctenidium and the mantle cavity, figuring in these pallial organs 63-65 %… More >

  • Open Access

    ARTICLE

    Fine-structural details of the photoreceptor membranes in the ocellus of the scale-insect parasite Centrodora sp. (Hymenoptera; Aphenelidae): A case of gene transfer between host and parasite?

    MOUYU YANG1, V.BENNO MEYER-ROCHOW1,2

    BIOCELL, Vol.28, No.2, pp. 151-154, 2004, DOI:10.32604/biocell.2004.28.151

    Abstract Only one insect (the scale insect Eriococcus sp.) is known, in which photoreceptive lamellae appear to have replaced the usual arthropod rhabdom microvilli. We are now reporting the presence of photoreceptive membranes, which also appear to resemble lamellae rather than microvilli, but they are in the ocellus of the tiny wasp Centrodora sp., which parasitizes scale insect eggs. The apparently optically homogenous lens of the Centrodora ocellus measures approximately 10 µm in diameter and, thus, operates at the limits of diffraction. We calculated that the lens is capable of focusing a parallel bundle of rays on the retina of the… More >

  • Open Access

    ARTICLE

    Probiotics enhance the recovery of gut atrophy in experimental malnutrition

    DIANA B. DOCK*, JOSÉ E. AGUILAR-NASCIMENTO**, MARCIA Q. LATORRACA*

    BIOCELL, Vol.28, No.2, pp. 143-150, 2004, DOI:10.32604/biocell.2004.28.143

    Abstract AIM: The aim of this study was to evaluate the effect of probiotics on the recovery of the bowel atrophy induced by malnutrition in rats. METHODS: Twenty-and-six Wistar rats (200-250g) were fed with either a normoproteic (sham group, n=6) or a free-protein diet (n=20) during 12 days. Twelve malnourished rats were randomized to recover during 15 days with either a hydrolyzed diet (control group, n=6) or the same diet enriched with probiotics (Streptococcus thermophilus and Lactobacillus helveticus; probiotic group, n=6). RESULTS: Probiotic group showed similar gain of body, liver and bowel weight than controls. At the jejunum, both the villus… More >

  • Open Access

    ARTICLE

    Study of the myenteric and submucous plexuses after BAC treatment in the intestine of rats

    NILZA CRISTINA BUTTOW, MIRIAM SANTIN, LUCIANA CONCI MACEDO, ALINE CRISTINA NERES TEIXEIRA, GISELE CAROLINE NOVAKOWSKI, TAÍSE ROBERTA BOLONHEIS ARMELIN, KATHYA ASSMANN

    BIOCELL, Vol.28, No.2, pp. 135-142, 2004, DOI:10.32604/biocell.2004.28.135

    Abstract A morphological and quantitative study in the ileal and colonic myenteric and submucous plexuses of rats after BAC denervation was performed. Four groups were employed: SI – ileum control; CBI – denervated ileum; SC – colon control; and CBC – denervated colon. We used the Myosin-V immunohistochemistry technique to study the myenteric and submucous plexuses. In the submucous plexus of the ileum and colon there was not a significant decrease in the number of neurons/mm2 and of ganglia/mm2 . The denervation of the myenteric plexus in the group CBI was 44.7% and in the group CBC, 68.3%. In the myenteric… More >

  • Open Access

    ARTICLE

    Histomorphological and quantitative immunohistochemical changes in the rat pancreas during aging

    F.L. RICCILLO, M.I. BRACAMONTE, G.M. CÓNSOLE, C.L.A. GÓMEZ DUMM

    BIOCELL, Vol.28, No.2, pp. 127-134, 2004, DOI:10.32604/biocell.2004.28.127

    Abstract Although the endocrine pancreas is the purpose of several deep investigations, morphological data referred to the effect of aging on the gland are not homogeneous. The purpose of the current work was to analyze the changes occurring in the pancreas of aged rats, with especial reference to the islet cell populations. Six young (Y), old (O) and senescent (S) male Sprague-Dawley rats were used. The pancreas tails were processed for light microscopy and studied by means of routine stains as well as by immunohistochemical identification of insulin-, glucagon-, somatostatin-, and pancreatic polypeptide- secreting cells (Dako Envision System, DAB as chromogen).… More >

  • Open Access

    ARTICLE

    Influence of genotype and explant source on indirect organogenesis by in vitro culture of leaves of Melia azedarach L.

    S.K. VILA*, H.Y. REY, L.A. MROGINSKI

    BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

    Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l-1 benzylaminopurine (BAP) + 0.1mg.l-1 kinetin (KIN) + 3 mg.l-1 adenine sulphate (ADS).
    The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the genotypes… More >

  • Open Access

    ARTICLE

    Bacteriostatic action of synthetic polyhydroxylated chalcones against Escherichia coli

    MARÍA DE LOS ANGELES ALVAREZ, VALERIA E. P. ZARELLI, NORA B.PAPPANO, NORA B. DEBATTISTA

    BIOCELL, Vol.28, No.1, pp. 31-34, 2004, DOI:10.32604/biocell.2004.28.031

    Abstract In previous work the bacteriostatic action of trihydroxylated chalcones against Staphylococcus aureus ATCC 25 923 was investigated. In this work the action of 2´, 4´, 2-(OH)3 -chalcone, 2´, 4´, 3-(OH)3- chalcone and 2´, 4´, 4-(OH)3 –chalcone against Escherichia coli ATCC 25 922 was evaluated. Growth kinetic curves of E.coli were made in nutritive broth added with increasing drug concentrations. The specific growth rates of the microorganisms were calculated by a kinetic turbidimetric method, which was previously probed and the minimal inhibitory concentrations (MIC´s) were evaluated by a mechanism of action proposed. The MICs of 2´, 4´, 3-(OH)3 -chalcone and 2´,… More >

  • Open Access

    ARTICLE

    Tissue alterations in the Guinea pig lateral prostate following antiandrogen flutamide therapy

    RENATO SIMÕES CORDEIRO*, WELLERSON RODRIGO SCARANO*, REJANE MAIRA GÓES**, SEBASTIÃO ROBERTO TABOGA**

    BIOCELL, Vol.28, No.1, pp. 21-30, 2004, DOI:10.32604/biocell.2004.28.021

    Abstract The flutamide antiandrogenic effects on the Guinea pig male prostate morphology in puberal, post-puberal and adult ages were evaluated in the present study. Daily-treated group animals received flutamide subcutaneous injection at a dose of 10 mg/Kg body weight for 10 days. The control group animals received a pharmacological vehicle under the same conditions. The lateral prostate was removed, fixed and processed for light and transmission electron microscopy. The results revealed an increase of the acinus diameter in the treated puberal animals and straitness in the stromal compartment around the acini. The epithelial cells exhibited cubic phenotype. In the post-puberal and… More >

  • Open Access

    ARTICLE

    Genetic stability in rice micropropagation

    R. MEDINA, M. FALOCI, M.A. MARASSI, AND L.A. MROGINSKI.

    BIOCELL, Vol.28, No.1, pp. 13-20, 2004, DOI:10.32604/biocell.2004.28.013

    Abstract An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l-1.… More >

  • Open Access

    REVIEW

    Review : Uterine mast cells: A new hypothesis to understand how we are born

    M. ISOLDE RUDOLPH1, I. GINA ROJAS2, ALICIA B. PENISSI3

    BIOCELL, Vol.28, No.1, pp. 1-11, 2004, DOI:10.32604/biocell.2004.28.001

    Abstract Birth is the result of complex, well-defined, and coordinated events, that are tightly regulated by endocrine, nervous, and immune responses, and take place primarily in the female reproductive tract. Various mechanisms and mediators involved in pregnancy, labor, and delivery, are highly conserved among different mammalian species and mast cells emerge as potential and crucial participants in these processes, as it is discussed in this review. More >

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