GLADYS N. HERMIDA, ALEJANDRO FARÍAS, LUISA E. FIORITO

BIOCELL, Vol.26, No.3, pp. 347-355, 2002, DOI:10.32604/biocell.2002.26.347

Abstract The lung of the toad, Melanophryniscus stelzneri stelzneri was studied using scanning and transmission electron microscopy. In M.s.stelzneri the parenchyma forms a polygonal network arrangement, therefore the parenchyma is edicular. These spaces are delimited by the interconnection of third order septa which are covered by respiratory epithelium. Small patches of ciliated epithelium without goblet cells appear irregularly distributed on the septa. The respiratory epithelium consists of one type of pneumocyte, which shows characteristics of both type I and type II alveolar cells of higher vertebrates. The pneumocytes are irregular in shape and possess attenuated cytoplasmic processes, which… More >

ESSI KESKINEN¹ , YASUHARU TAKAKU² , V. BENNO MEYER-ROCHOW³, TAKAHIKO HARIYAMA⁴

BIOCELL, Vol.26, No.3, pp. 357-367, 2002, DOI:10.32604/biocell.2002.26.357

Abstract The aims of this paper have been (a) to characterize marginal ommatidia from different eye regions through a detailed description of their distinct ultrastructural features in three different size-classes of L. exotica, and (b) to compare microanatomical characteristics of the marginal ommatidia with those of ommatidia of the same eye, but located further centrally. On the basis of transverse as well as longitudinal sections we conclude that new ommatidia are added from a crescentic dorso-anterio-ventral edge of the eye and that maturing ommatidia go through a sequence in which originally the nuclei of cone -,… More >

A. VIGLIOCCO^*, B. BONAMICO^*, S. ALEMANO^*, O. MIERSCH^**, G. ABDALA^*

BIOCELL, Vol.26, No.3, pp. 369-374, 2002, DOI:10.32604/biocell.2002.26.369

Abstract In the present paper we study the possible biological relevance of endogenous jasmonic acid (JA) and exogenous salicylic acid (SA) in a plant-microbial system maize-virus. The virus disease "Mal de Río Cuarto" is caused by the maize rough dwarf virus - Río Cuarto. The characteristic symptoms are the appearance of galls or "enations" in leaves, shortening of the stem internodes, poor radical system and general stunting. Changes in JA and protein pattern in maize control and infected plants of a virus-tolerant cultivar were investigated. Healthy and infected-leaf discs were collected for JA measurement at… More >

CHARLES GÉMINARD, AUDE DE GASSART, MICHEL VIDAL

BIOCELL, Vol.26, No.2, pp. 205-215, 2002, DOI:10.32604/biocell.2002.26.205

Abstract During the differentiation of erythroid cells, a vast program of maturation takes place, leading to decay or elimination of organelles, including the nucleus, mitochondria, ribosomes, lysosomes, endoplasmic reticulum and Golgi apparatus. During the last step of red cell maturation, remaining organelles, primarily mitochondria and ribosomes but also vestiges of others are finally cleared from the cell. This cleaning session also affects specific proteins that are partially or entirely removed from the cell surface. The interplay of the various events and their causal relationships are approached here. More >

MARIANA N. MELCHIORRE, HERNÁN R. LASCANO, VICTORIO S.TRIPPI

BIOCELL, Vol.26, No.2, pp. 217-223, 2002, DOI:10.32604/biocell.2002.26.217

Abstract Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embriogenic calli generation from inmature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene β-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by β-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other More >

ALICIA I. WEYERS, LAURA I. UGNIA, HUGO GARCÍA OVANDO, NORA B. GORLA*

BIOCELL, Vol.26, No.2, pp. 225-228, 2002, DOI:10.32604/biocell.2002.26.225

Abstract Ciprofloxacin (CFX) is an effective and relatively safe antimicrobial used in a variety of human infections. However, adverse drug reactions and positive results in genotoxic tests are reported.
In order to understand the possible pathophysiological mechanisms of the toxic effects informed for CFX, lipid hydroperoxides (LOOH) -oxidative mediators of peroxidation- were quantified in liver and kidney of mice, after 15 to 360 minutes of the ciprofloxacin administration at doses of 10 mg/ Kg or 100 mg/ Kg by ip route. The peroxidation in the lipid fraction was evaluated by measuring the amount of hydroperoxides through… More >

DANIELA CARVALHO DOS SANTOS, ELISA APARECIDA GREGÓRIO

BIOCELL, Vol.26, No.2, pp. 229-235, 2002, DOI:10.32604/biocell.2002.26.229

Abstract The ultrastructure of the ovariole sheath along the Diatraea saccharalis ovariole was studied by scanning and transmission electron microscopy. Each ovariole is surrounded by an epithelial sheath, a tunica propria and scattered lumen cells. These three components of the ovariole sheath show different ultrastructural features along the ovariole, in the germarium or in the vitellarium; these differences are more evident in the epithelial sheath cells. The epithelial sheath is composed by two layers of cells, the external one running longitudinally and the internal one running circularly in the ovariole. These cells, in vitellarium, present cytoplasmic bundles More >

LIDIA M. LOPEZ^*, AMANDA PELLEGRINO DE IRALDI^*, PATRICIA H. CARRIZO^**, MARTA DUBIN^**, ANDRÉS O.M. STOPPANI^**

BIOCELL, Vol.26, No.2, pp. 237-245, 2002, DOI:10.32604/biocell.2002.26.237

Abstract CG 10-248 (3,4-dihydro-2,2 dimethyl-9-chloro-2H-naphtho[1,2b]pyran-5,6-dione), a ß-lapachone analogue, modified the ultrastructure of rat liver mitochondria in vitro, in the absence of added oxidizable substrates. The condensed mitochondrial state was replaced by the orthodox or swollen state to a significant degree. The number of modified mitochondria depended on incubation time and quinone concentration, in the 25-100 µM range. Under the same experimental conditions, mitochondrial respiration was uncoupled as indicated by the increase in the rate of succinate oxidation by controlled mitochondria in metabolic state “4” (not in state “3”), and by the activation of latent F₀ F₁ -ATP synthase. More >

JUAN J. LÓPEZ-COSTA, CAROLINA ACUIPIL, PATRICIA TAGLIAFERRO, SAMEER PATEL, JAVIER RAMOS, ALICIA BRUSCO, JORGE PECCI SAAVEDRA*

BIOCELL, Vol.26, No.2, pp. 247-252, 2002, DOI:10.32604/biocell.2002.26.247

Abstract NADPH-diaphorase is a useful technique to reveal NO producing neurons at light microscopic level (LM). A modification of the technique using the tetrazolium salt BSPT as susbtrate, is useful to study the ultrastructure of NO neurons. The aim of this work was to perform a detailed analysis of NADPH diaphorase reactive neurons in rat mesencephalon both at light and electron microscopic levels.
NADPH-diaphorase reactive neurons were observed in superior colliculus, in central gray matter, in dorsal and medial raphe and in the pedunculopontine tegmental nucleus using two histochemical techniques at LM. Electron microscopy showed deposits More >

ARTURO D. JORGE^*, LUIS S. GUTIERREZ^**, OLIVER JORGE^*, MARIO H. BURGOS^**

BIOCELL, Vol.26, No.2, pp. 253-262, 2002, DOI:10.32604/biocell.2002.26.253

Abstract The aim of this paper is to establish whether there are cytochemical or ultrastructural alterations in the hepatocytes of patients with primary biliary cirrhosis (PBC) at stages I and II compared with the biopsies from individuals with normal liver. Cytochemical technique with ATP as substrate, transmission electron microscopy (TEM) and freeze fracture were used for the studies.
In the normal liver biopsies the ultrastructural cytochemical localization of the enzymatic activity was clearly shown in the bile canaliculi. In the PBC biopsies, the enzymatic activity is increased in the bile canaliculi and is also present… More >